ZHAO Lin-lin,HAO Xin,LI Tong,et al.The Effect of Tea Polyphenols on Oxidative Damage of Pancreas in Mice Induced by Soybean Trypsin Inhibitor[J].Soybean Science,2018,37(06):950-954.[doi:1011861/jissn1000-98412018060950]
茶多酚对大豆胰蛋白酶抑制因子致小鼠胰腺氧化损伤的缓解作用
- Title:
- The Effect of Tea Polyphenols on Oxidative Damage of Pancreas in Mice Induced by Soybean Trypsin Inhibitor
- Keywords:
- Tea polyphenols; Soybean trypsin inhibitor; Pancreas; Serum; Free radical; Mice
- 文献标志码:
- A
- 摘要:
- 为研究茶多酚(TP)对大豆胰蛋白酶抑制因子(STI)致小鼠胰腺氧化损伤的缓解作用。试验设对照组(control组)、模型组（STI组，于日粮中添加36 mg·g-1的STI）、茶多酚组(TP组，于日粮中添加1000 mg·kg-1的茶多酚和36 mg·g-1的STI）。第21天处死小鼠，研究处理组间胰腺和血清中丙二醛(MDA)、总抗氧化能力(T-AOC)、几种消化酶和激素含量的变化，并通过透射电镜观察胰腺超微结构变化。结果表明：与对照组相比，模型组小鼠胰腺和血清中的丙二醛、生长抑素(SS)、淀粉酶(AMS)、脂肪酶(LPS)含量均显著升高(P<005)，T-AOC、胰岛素(INS)和胰蛋白酶(TPS)含量显著降低(P<005)，线粒体损伤严重；而添加茶多酚的TP组较STI组，MDA、SS、AMS、LPS含量均显著降低(P<005)，T-AOC、INS和TPS含量显著升高(P<005)，线粒体有轻微损伤。表明茶多酚有效抑制了STI诱导的氧化应激，缓解了STI造成的胰腺氧化损伤。
- Abstract:
- In order to assess the effect of tea polyphenols on oxidative damage of pancreas in mice induced by soybean trypsin inhibitor factor(STI), 36 male mice were randomly divided into three groups: Control group, model group(STI group, feeding the daily ration of 36 mg·g-1 STI) and tea polyphenols group (TP group, feeding the daily ration of 1000 mg·kg-1 tea polyphenols and 36 mg·g-1 STI) Mice were executed on 21 d and their malonaldehyde(MDA), total antioxidant capacity(T-AOC), several digestive enzyme and hormone contents of pancreas and serum were determined, ultrastructural changes of pancreas were observed by TEM As shown in results, compared with control group, the MDA, somatostatin(SS), amylase(AMS) and lipase(LPS) contents in the pancreas and serum in STI group were significantly increased (P<005) , T-AOC, trypsin(TPS) and insulin(INS) were significantly decreased (P<005), mitochondria were severely damaged In the TP group with the addition of tea polyphenol, the MDA, SS, AMS and LPS contents in the pancreas and serum were significantly decreased (P<005) , T-AOC, TPS and INS were significantly increased (P<005), mitochondria were slightly damaged This study showed that tea polyphenols effectively inhibited the oxidative stress induce by STI, weakened the oxidative damage of pancreas
参考文献/References:
［1］李单, 田河, 陈山, 等 大豆主要抗营养因子对仔猪影响的研究进展［J］. 饲料研究, 2015, 23(3): 10-13 (Li D, Tian H, Chen S Research progress on the effect of soybean anti-nutritional factors on piglets［J］. Feed Research, 2015, 23(3): 10-13)
［2］谷春梅, 韩玲玲, 曲洪生, 等 大豆胰蛋白酶抑制因子对小鼠胰腺氧自由基水平的影响 ［J］. 中国兽医学报, 2013, 33(12): 1902-1906 (Gu C M, Han L L, Qu H S, et al. The effect of soybean trypsin inhibitor on oxygen free radical level in pancreas of mice［J］. Chinese Journal of Veterinary Science, 2013, 33(12): 1902-1906）
［3］Gu C M, Zhao L L, Han L L, et al. The effect of soybean trypsin inhibitor on the generation of oxygen free radical in mice during different growth periods ［J］. Food Science and Technology Research, 2014, 20: 431-438）
［4］谷春梅, 施用晖, 乐国伟高蛋白膳食对小鼠胰腺功能的氧化损伤［J］.中国畜牧兽医, 2010, 37 (11): 32-35 (Gu C M, Shi Y H, Le G W Oxidative damage of high protein diet to pancreatic function in mice［J］. China Animal Husbandry & Veterinary Medicine, 2010, 37 (11): 32-35 )
［5］赵琳琳, 解慧, 张彪, 等 茶多酚对大豆胰蛋白酶抑制因子诱导氧化应激的抑制作用［J］.大豆科学, 2018, 37 (3): 373-377（Zhao L L, Xie H, Zhang B, et al. The inhibitory effect of tea polyphenols on the oxidative stress induced by soybean trypsin inhibitor［J］.Soybean Science, 2018, 37 (3):373-377）
［6］Biswas A H, Wakita M Effect of dietary Japanese green tea powder supplementation on feed utilization and carcass profiles in broilers［J］. The Journal of Poultry Science, 2001, 38 (1): 50-57
［7］Tao J, Shen X H, Ai Y H, et al. Tea polyphenols protect against ischemia/reperfusion-induced liver injury in mice through anti-oxidative and anti-apoptotic properties［J］. Experimental and Theraperapeutic Medicine, 2016, 12 (5): 3433-3439
［8］Kulandaivelu K, Mandal A K Positive regulation of bio-chemical parameters by tea polyphenol encapsulated solid lipid nanoparticles at in vitro and in vivo conditions ［J］. Inset Engineering Technology Nanobiotechnology, 2016, 10(6): 419-424
［9］李淑君 大豆胰蛋白酶抑制因子致胰腺线粒体氧化损伤机理的研究［D］. 长春：吉林农业大学，2015(Li S J Study on the mechanism of pancreatic mitochondrial oxidative damage induced by soybean trypsin inhibitor［D］. Changchun: Jilin Agricultural University，2015)
［10］赵磊, 高民, 马燕芬 茶多酚的抗氧化作用及其机制［J］. 动物营养学报, 2017, 29 (6): 1861-1865 (Zhao L, Gao M, Ma Y F Anti-oxidation functions of tea polyphenols and their mechanisms［J］. Chinese Journal of Animal Nutrition, 2017, 29 (6): 1861-1865 )
［11］Song J L, Qian Y, Li G J, et al. Anti-inflammatory effects of kudingcha methanol extract(Ilex kudingcha CJ Tseng) in dextransulfate sodium-induced ulcerative colitis［J］. Molecular Medicine Reports, 2013, 8(4): 1256-1262
［12］赵欣, 李贵节, 胡园园, 等 苦丁茶多酚提取物对四氯化碳诱导小鼠肝损伤的改善作用及机制研究［J］. 营养与保健, 2018, 39（4）: 289-295 （Zhao X, Li G J, Hu Y Y, et al. Improment effects and mechanism research of polyphenol extracts from Kudingcha on carbon tetrachloride induced hepatic damage in mice［J］. Science and Technology of Food Industry, 2018, 39（4）: 289-295）
［13］王妍琪 茶多酚、VE对肉用仔鸡热应激时生产性能和抗氧化性能的影响［D］. 哈尔滨：东北农业大学, 2004 (Wang Y Q Effect of tea polyphenols and vitamin E on broilers performance and antioxidant on under heat-stress conditions［D］. Harbin: Northeast Agricultural University, 2004)
［14］Zhong R Z, Tan C Y, Han X F, et al. Effect of dietary tea catechins supplementation in giats on the quality of meat kept under refrigeration［J］. Small Ruminant Research, 2009, 87(1): 122-125
［15］蔡海莹, 徐晓娟, 张磊, 等 日粮中添加茶多酚对肉鸡抗氧化性能及免疫器官指数的影响［J］. 中国家禽, 2010, 32(13): 11-14 (Cai H Y, Xu X J, Zhang L, et al. Effect of tea polyphenol on antioxidation capacity and immune organs index of broilers［J］. China Poultry, 2010, 32(13): 11-14)
［16］Kurt , Demirci H, Ozturk K, et al. Severe serum amylase elevation, with only chronic kidney disease［J］. Renal Failure, 2015, 37(5):915
［17］曹友德, 陈玮, 李浩 急性胰腺炎患者脂肪酶的检测与临床意义［J］. 中国现代医学杂志, 2001, 11(6): 90-91 (Cao Y D, Chen W, Li H Detection and clinical significance of lipase in patients with acute pancreatitis［J］. China Journal of Modern Medicine, 2001, 11(6): 90-91 )
［18］蒋品 淀粉酶在胰腺炎诊断中的作用［J］. 实用医技杂志, 2004, 11(12): 2636-2637(Jiang P The role of amylase in the diagnosis of pancreatitis［J］. Journal of Practical Medical Techniques, 2004, 11(12): 2636-2637)
［19］杨志刚, 王宝维, 王雷 大豆胰蛋白酶抑制因子的研究进展［J］. 饲料博览, 2005(12): 31-33(Yang Z G, Wang B W, Wang L Research progress of soybean trypsin inhibitor［J］. Feed Review, 2005(12): 31-33)
［20］Liang H H, Huang H H, Kwok K C Properties of tea polyphenol complexed bromelain［J］. Food Research International, 1999, 32(8): 545-551
［21］赵自刚, 牛春雨, 侯亚利,等 兔急性肾功能衰竭时胰腺形态与功能及自由基的变化［J］. 中国病理生理杂志, 2005, 21 (8): 1599-1601（Zhao Z G，Niu C Y，Hou Y L , et al. Pancreatic morphology and function and free radicals in rabbits with acute renal failure［J］. Chinese Journal of pathophysiology, 2005, 21 (8): 1599-1601）
［22］唐亚丽, 卢立新, 王军 生长抑素与消化系统的氧化损伤［J］. 饲料工业, 2009, 30 (23): 48-51(Tang Y L, Lu L X, Wang J Somatostatin and oxidative damage of digestive system［J］. Feed Industry, 2009, 30 (23): 48-51)
相似文献/References:
[1]谷春梅,韩玲玲,曲洪生,等.大豆胰蛋白酶抑制因子的研究进展[J].大豆科学,2012,31(01):149.[doi:10.3969/j.issn.1000-9841.2012.01.034]
GU Chun-mei,HAN Ling-ling,QU Hong-sheng,et al.Recent Advances in Soybean Trypsin Inhibitor[J].Soybean Science,2012,31(06):149.[doi:10.3969/j.issn.1000-9841.2012.01.034]
[2]赵琳琳,解慧,张彪,等.茶多酚对大豆胰蛋白酶抑制因子诱导氧化应激的抑制作用[J].大豆科学,2018,37(03):373.[doi:10.11861/j.issn.1000-9841.2018.03.0373]
ZHAO Lin-lin,XIE Hui,ZHANG Biao,et al.The Inhibitory Effect of Tea Polyphenols on the Oxidative Stress Induced by Soybean Trypsin Inhibitor[J].Soybean Science,2018,37(06):373.[doi:10.11861/j.issn.1000-9841.2018.03.0373]
备注/Memo
收稿日期：2018-05-28