[1]杨可欣,王欢,刘雪娇,等.生防芽孢杆菌8-32对盆栽大豆土壤酶系和微生物区系的影响[J].大豆科学,2018,37(05):762-768.[doi:10.11861/j.issn.1000-9841.2018.05.0762]
　YANG Ke-xin,WANG Huan,LIU Xue-jiao,et al.Effect of Bacillus Subtilis 8-32 on Soil Enzymes and Microflora of Potted Soybean[J].Soybean Science,2018,37(05):762-768.[doi:10.11861/j.issn.1000-9841.2018.05.0762]
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生防芽孢杆菌8-32对盆栽大豆土壤酶系和微生物区系的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷 期数: 2018年05期 页码: 762-768 栏目: 出版日期: 2018-09-20
Title:
Effect of Bacillus Subtilis 8-32 on Soil Enzymes and Microflora of Potted Soybean
作者:
（河北农业大学 生命科学学院，河北 保定 071000）
Author(s):
YANG Ke-xin;  WANG Huan;  LIU Xue-jiao;  SHI Xiang-zhe;  JIA Tian-hui;  GAO Tong-guo
(College of Life Science, Agricultural University of Hebei, Baoding 071001, China)
关键词:
大豆根腐病; 生物防治; Bacillus subtilis; 土壤酶系; 微生物区系
Keywords:
Soybean root rot;  Biocontrol;  Bacillus subtilis;  Soil enzymes;  Microflora
DOI:
10.11861/j.issn.1000-9841.2018.05.0762
文献标志码:
A
摘要:
前期研究中筛选到一株对大豆根腐病病原菌-尖孢镰刀菌具有较强拮抗作用的菌株Bacillus subtilis 8-32，其生防效果达32.08%。本试验采用盆栽法，研究接种不同浓度枯草芽孢杆菌8-32（Bacillus subtilis 8-32）后，土壤酶系和微生物区系的变化情况。结果表明：使用枯草芽孢杆菌8-32后土壤脲酶活性增加了16.9%~66.7%，蛋白酶活性增加了88.8%~131.0%，酸性磷酸酶活性增加了20.6%~51.8%，碱性磷酸酶活性增加了7.5%~14.6%，根际土壤中细菌数量增加8.5%~72.5%，对土壤中真菌数量抑制率最高达37.2%，放线菌数量也有明显增加。说明菌株8-32对由尖孢镰刀菌引起的大豆根腐病有显著的防治效果，具有进一步应用的潜力。
Abstract:
In previous study, a strain Bacillus subtilis 8-32, which had strong antagonistic ability against to Fusarium oxysporum was screened, its biocontrol effect reached to 32.08%. In this experiment, further study was carried out by pot experiments. We studied the changing of soil enzyme system and microflora after inoculated different concentration of Bacillus subtilis 8-32. The results showed that: Bacillus subtilis 8-32 increased the activity of soil urease by 16.9%-66.7%, protease activity increased by 88.8%-131.0%, acid phosphatase activity increased by 20.6%-51.8%, alkaline phosphatase activity increased by 7.5%-14.6% and bacteria in soil increased by 8.5%-72.5%, the quantity of soil fungi inhibition rate reached to 37.2%, the number of actinomyces increased obviously. It indicated that strain 8-32 has significant control effect on soybean root rot caused by Fusarium oxysporum, and has potential for further application.

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备注/Memo

收稿日期：2018-04-29

基金项目：河北农业大学教改项目（2015JM04）；河北农业大学大学生创新创业训练计划（2018156）；河北省教育厅拔尖人才项目（BJ2016029）；河北自然科学基金青年项目（C2015204031）；河北省教育厅课题“生物技术”品牌特色专业建设研究项目（2012GJJG054）；教育部、河北省教育厅“生物技术”专业综合改革试点项目（201202）。
第一作者简介：杨可欣（1998-），女，学士，主要从事植物真菌病害研究。E-mail: 1679831908@qq.com。
通讯作者：高同国（1984-），男，博士，讲师，主要从事植物真菌病害生物防治研究。E-mail: gtgrxf@163.com。

更新日期/Last Update: 2018-10-08