[1]褚盼盼,靳泽荣,乔元彪.黑豆皮花青素的不同提取方法及其体外抗菌活性比较研究[J].大豆科学,2015,34(04):685-689.[doi:10.11861/j.issn.1000-9841.2015.04.0685]
　CHU Pan-pan,JIN Ze-rong,QIAO Yuan-biao.Comparison of Different Extraction Methods of Black Soybean Skin Anthocyanin and Its Antibacterial Activity in vitro[J].Soybean Science,2015,34(04):685-689.[doi:10.11861/j.issn.1000-9841.2015.04.0685]
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黑豆皮花青素的不同提取方法及其体外抗菌活性比较研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年04期 页码: 685-689 栏目: 出版日期: 2015-08-25
Title:
Comparison of Different Extraction Methods of Black Soybean Skin Anthocyanin and Its Antibacterial Activity in vitro
作者:
褚盼盼; 靳泽荣; 乔元彪
吕梁学院，山西 吕梁 033000
Author(s):
CHU Pan-pan; JIN Ze-rong; QIAO Yuan-biao
Lyuliang University, Lyuliang 033000,China
关键词:
黑豆皮; 花青素; 提取; 抑菌圈; 抑菌率
Keywords:
Black soybean skin; Anthocyanins; Extraction; Bacteriostatic ring; Antibacterial rate
DOI:
10.11861/j.issn.1000-9841.2015.04.0685
文献标志码:
A
摘要:
对黑豆皮花青素不同提取方法，传统有机溶剂法、水浸提法、超声波辅助法的提取率进行了比较，结果表明：超声波辅助法的提取率最高，达到20.50%。并采用滤纸片法对3种不同提取方法所得的花青素抑菌效果进行了比较，结果显示：水浸提法对枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌3种细菌的抑菌圈均最大，抑菌效果最好。另采用平板菌落计数法对水浸提法所得的花青素抑菌率进行比较发现，从低浓度到高浓度抑菌效果逐渐增强。当花青素在浓度为0.3 g.mL-1时，对枯草芽孢杆菌、金黄色葡萄球菌和大肠杆菌3种细菌的抑菌率最高，分别达到79.75%、74.50%和81.60%。
Abstract:
The extraction yield of different extraction methods of anthocyanin in black soybean skin inculding the traditional method of organic solvent, water extraction,ultrasonic auxiliary method were compared, method of ultrasonic assisted extraction yield was the highest,reached 20.50%.And by the method of filter,three different extraction methods of the bacteriostatic effect of anthocyanins were compared.The result showed that the bacteriostatic ring of the water extraction of Bacillus subtilis,Staphylococcus aureus,Escherichia coli was the largest and the bacteriostasis effect was the best.The anthocyanin bacteriostatic rate compared by the plate colony counting method of water extraction showed that from low concentration to high concentration bacteriostatic effect was increased.When anthocyanins in concentration was 0.3 g.mL-1,Bacillus subtilis,Staphylococcus aureus,Escherichia coli antibacterial rate was 79.75%,74.50% and 81.60%, respectively.

参考文献/References:

［1］朱宏达，张慧，张美荣，等.黑豆红色素的提取工艺及其理化性质的研究［J］中国食品添加剂，2009(1)：86-90（Zhu H D, Zhang H, Zhang M R,et al.The study of black bean red extracting from black soybean and its physical and chemical properties［J］.China Food Additives，2009(1)：86-90）

［2］Choung M G，Baek I Y，Kang S T，et al.Isolation and determination of anthocyanins in seed coats of black soybean(Glycine max L Merr)［J］Journal of Agricultural and Food Chemistry,2001，49(12)：5848-5851
［3］肖怀秋，李玉珍.黑豆皮中红色色素萃取工艺的响应面优化［J］.氨基酸和生物资源，2010，32(2)：30-34（Xiao H,Li Y Z.Optimization of extraction technology of red pigment from BSC using response surface methodology［J］.Amino Acids & Biotic Resources，2010，32(2)：30-34）
［4］杨卫民,刘保琪,李团霞，等.黑豆种皮中红色素的提取及稳定性研究［J］.大豆科学，2010,29（4）:688-691（Yang W M,Liu B Q,Li T X,et al.Extraction of red pigments from seedcoat of black soybean and its stability［J］.Soybean Science,2010,29(4):688-691）
［5］王少波.黑豆皮中花色苷的提取及纯化研究［D］西安：西安理工大学，2008.（Wang S B.Study on extraction and purification process of anthocyanins from black bean peal［D］Xi′an: Xi′an University of Technology,2008）
［6］李大婧，宋江峰，刘春泉，等.超声波辅助提取黑豆皮色素工艺优化［J］.农业工程学报，2009，25(2)：273-279（Li D J,Song J F,Liu C Q,et al.Optimization of technology for ultrasonic-assisted extraction of pigments from black soybean hulls［J］.Transactions of the Chinese Society of Agricultural Engineering，2009，25(2)：273-279）
［7］高雪琴，王若兰，杨建平.黑豆皮红色素微波辅助提取与乙醇浸提的工艺比较研究［J］食品与药品，2006(5):39-41.（Gao X Q,Wang R L,Yang J P.A comparison study on extraction technology of black bean peal red pigment between microwaveassisted extraction and traditional extraction method［J］Food and Drug，2006(5):39-41）
［8］Xu B J，Chang S K C.Antioxidant capacity of seed coat, dehulled bean, and whole black soybeans in relation to their distributions of total phenolics, phenolic acids, anthocyanins, and isoflavones［J］.Journal of Agricultural and Food Chemistry,2008,56(18),8365-8373
［9］滕学峰.黑豆皮中色素的提取纯化、结构鉴定及其性质的研究［D］哈尔滨：东北农业大学，2008（Teng X F.The extraction,refinement structural identification property of pigment compoud in the coat of black soybean［D］. Harbin: Northeast Agricultural University,2008）
［10］张芳轩，张名位，张瑞芬，等.不同黑大豆种质资源种皮花色苷组成及抗氧化活性分析［J］.中国农业科学,2010,43(24):5088-5099（Zhang F X,Zhang M W,Zhang R F,et al.Comparative analysis of the anthocyanin profiles and antioxidant activity of seed coats from different black soybean germplasm resources［J］Scientia Agricultura Sinica，2010,43(24):5088-5099）
［11］张花利，冯进，董晓娜，等.黑豆皮中黄酮提取及粗提物抑菌效果研究［J］.大豆科学，2001，30（3）：497-501（Zhang H L,Feng J,Dong X N,et al.Extraction of flavonoids from the peel of black bean and bacteriostatic effect of crude extractings［J］.Soybean Science,2011,30(3):497-501）
［12］钱存柔，黄仪秀.微生物学实验教程［M］.北京：北京大学出版社，2000.（Qian C R, Huang Y X.Microbiology experiment course［M］.Beijing: Peking University Press, 2000）

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备注/Memo

基金项目：山西省高等学校科技创新项目(2013168)；山西省自然科学基金（2012011002-3）。

第一作者简介：褚盼盼（1982-），女，硕士，讲师，主要从事植物生理活性物质的研究与开发。E-mail:chupanpan@sohu.com。
通讯作者：乔元彪（1970-），男，博士，副教授，主要从事药物化学研究。E-mail:qyb_0222@sina.com。

更新日期/Last Update: 2015-09-01