[1]张诗尧,赵元,刘丹丹,等.日粮中大豆抗原蛋白检测方法的研究进展[J].大豆科学,2015,34(03):518-521.[doi:10.11861/j.issn.1000-9841.2015.03.0518]
　ZHANG Shi-yao,ZHAO Yuan,LIU Dan-dan,et al.Reviews on the Detection Methods of Soybean Allergic Proteins in Diets[J].Soybean Science,2015,34(03):518-521.[doi:10.11861/j.issn.1000-9841.2015.03.0518]
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日粮中大豆抗原蛋白检测方法的研究进展

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年03期 页码: 518-521 栏目: 出版日期: 2015-06-25
Title:
Reviews on the Detection Methods of Soybean Allergic Proteins in Diets
作者:
张诗尧1; 赵元1; 刘丹丹1; 鲍男1; 赵寒冬2; 张迪1
1.吉林农业大学 动物科学科技学院/动物生产及产品质量安全教育部重点实验室/吉林省动物营养与饲料科学重点实验室,吉林 长春 130118；
?2.长春农业博览园，吉林 长春 130118
Author(s):
ZHANG Shi-yao1;  ZHAO Yuan1;  LIU Dan-dan1;  BAO Nan1;  ZHAO Han-dong2;  ZHANG Di
1.College of Animal Science and Technology, Jilin Agricultural University/Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Changchun 130118, China; 2.Changchun Agriculture Expo Park, Changchun 130118, China
关键词:
大豆抗原蛋白; 竞争ELISA; 双抗夹心ELISA; 检测方法
Keywords:
Soybean antigen protein;  competitive ELISA;  Double-antibody sandwich ELISA;  Detection
DOI:
10.11861/j.issn.1000-9841.2015.03.0518
文献标志码:
A
摘要:
大豆抗原蛋白对动物造成不利的影响和不易灭活的特性，限制了大豆及其制品在动物饲料行业的应用。因而在当前蛋白质资源短缺的形式下，如何快速、简便、准确地检测出大豆中的抗原蛋白成为亟需解决的重要问题。文中综述了几种有关大豆抗原蛋白的常用检测方法，并比较其检测效果，为生产中选择适当的测定方法提供理论依据。
Abstract:
Soybean allergic protein may lead to several negative effects to animals, combined with the difficulty of its inactivated features, which become a bottleneck of animal nutrition industry.Thus, under the circumstance of shortage of the protein resource, the fast, simple and accurate detection methods of soybean allergic protein become an important problem which needs to be solved.In this paper, we reviewed and compared several detection methods of soybean allergic protein and provided a basis for selecting an appropriate detection method.

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备注/Memo

基金项目：国家自然科学基金青年基金(31101719)；吉林省科技发展计划项目青年科研基金（201201098）；国家“十二五”科技支撑计划(2013BAD17B0); 吉林省教育厅“十二五”科学技术研究规划项目(2015198)。

第一作者简介：张诗尧（1991-）女，在读硕士，主要从事动物营养与饲料科学研究。E-mail：1151352731@qq.com。
通讯作者：赵元(1981-)女，博士，副教授，主要从事饲料抗营养因子的研究。E-mail：zhaoyuan4CL52@126.com。

更新日期/Last Update: 2015-07-16