CHEN Wen-jie,CHEN Yuan,WEI Qing-yuan,et al.Seed-flooding Tolerance Identification and Comparison of Soybean Germplasm Resources in Southern China[J].Soybean Science,2020,39(02):165-173.[doi:10.11861/j.issn.1000-9841.2020.02.0165]
南方大豆种质种子耐浸水能力鉴定与分析
- Title:
- Seed-flooding Tolerance Identification and Comparison of Soybean Germplasm Resources in Southern China
- 文献标志码:
- A
- 摘要:
- 为鉴定比较南方不同大豆种质的耐浸水能力，为大豆种子耐浸水育种提供科学依据，本研究通过模拟田间渍害条件，对200份来自湖南等7省的南方大豆种质资源进行浸水处理120 h，然后进行标准发芽试验，记录各种质浸泡液电导率（CON120）、发芽势（GPP）、轻微损伤苗率（SISP）、发芽率（GP）和正常苗率（NSP）。采用基于主成分分析的综合指标法建立大豆种质耐浸水综合得分公式，用逐步回归分析筛选耐浸水关键指标，并建立耐浸水能力预测回归方程，计算各种质耐浸水能力值。研究结果表明：浸水处理后，200份南方大豆种质资源的CON120、GPP、SISP、GP、NSP均存在丰富的遗传变异，主成分分析提取两个主成分贡献率分别为64.03%和21.46%，逐步回归分析筛选出CON120、SISP、GP、NSP 4个耐浸水关键指标。不同来源地的大豆种质耐浸水能力存在差异，海南和广西大豆种质的综合耐浸水平均值较高，U值分别为0.885和0.748。综合指标法鉴定出ZDD16876等15份耐浸水能力强的大豆种质，来自广西、广东和湖南的大豆种质分别占40.00%、20.00%和20.00%。地方品种中，广东耐浸水种质比率高，广西次之，育成品种（品系）中，海南耐浸水种质比率最高。鉴定出的耐浸水种质将为今后大豆耐浸水育种提供材料基础。
- Abstract:
- In order to identify and compare the submergence tolerance of different soybean germplasm in southern China and provide scientific basis for submergence tolerance breeding of soybean seeds, in this study 200 soybean germplasm resources (consisted of 160 landraces and 40 cultivars) coming from seven southern provinces of China were seed-flooding treated for 120 h. And then standard germination test was conducted.We counted the electrical conductivity of soaking solution after treated for 120 h (CON120), measured the germination potential percentage (GPP), the slightly injured seedling percentage (SISP), the germination percentage (GP), and the normal seeding percentage (NSP). Comprehensive tolerance index based on principal component analysis was used to establish scoring formula, stepwise regression analysis was used to select key indicators. And then, prediction regression equation of water resistance was established, soybean seed flooding tolerance values were calculated. The results showed that there were abundant genetic variations in CON120, GPP, SISP, GP, NSP of 200 soybean germplasm resources. Two principal components were extracted, with contribution ratio 64.03% and 21.46% respectively. Seed-flooding tolerance ability of soybean germplasm resources form different provinces were different. The mean value of comprehensive seed-flooding tolerance of soybean from Hainan and Guangxi province was higher than others, with average U value 0.885 and 0.748 respectively. 15 soybean germplasms with high seed-flooding tolerance, including ZDD16876 et al., were screened out by using comprehensive index. The 40, 20 and 20 percent of the 15 high seed-flooding tolerance germplasms were from Guangxi, Guangdong and Hunan province respectively. In the land races, seed-flooding tolerance ratio of soybean from Guangdong was the highest, followed by Guangxi. In the varieties, seed-flooding tolerance ratio of soybean from Hainan was the highest. These high seed-flooding tolerance soybean germplasm resources could be used for seed-flooding tolerance breeding.
参考文献/References:
［1］王芳, 赵团结, 喻德跃, 等. 大豆苗期耐淹性的遗传与QTL分析［J］. 作物学报, 2008, 34(5): 748-753.（Wang F, Zhao T J, Yu D Y, et al. Inheritance and QTL analysis of submergence tolerance at seedling stage in soybean Glycine max (L.) Merr［J］. Acta Agronomica Sinica, 2008, 34(5): 748-753.）［2］孙慧敏, 赵团结, 盖钧镒. 大豆苗期耐淹性的遗传与QTL分析［J］. 作物学报, 2010, 36(4): 590-595.(Sun H M, Zhao T J, Gai J Y. Inheritance and QTL mapping of waterlogging tolerance at seedling stage of soybean［J］. Acta Agronomica Sinica, 2010, 36(4): 590-595.)［3］Vantoai T T, Beuerlein A F, Schmitthenner S K, et al. Genetic variability for flooding tolerance in soybeans［J］. Crop Science, 1994, 34(4): 1112-1115.［4］陈文杰，汤复跃，梁江，等. 大豆耐涝研究进展［J］. 南方农业学报, 2012, 43(2): 191-195. (Chen W J, Tang F Y, Liang J, et al. Research progress on waterlogging tolerance in soybean［J］. Journal of Southern Agriculture, 2012, 43(2):191-195.)［5］Wu C J, Chen P G, Hummer W,et al. Effect of flood stress on soybean seed germination in the field［J］. American Journal of Plant Sciences, 2017, 8: 53-68.［6］Hou F F, Thseng F S. Studies on the screening technique for pre-germination flooding tolerance in soybean［J］. Japan Crop Science, 1992, 61(3): 447-453.［7］Hou F F, Thseng F S. Studies on the flooding tolerance of soybean seed: Varietal differences［J］. Euphytica, 1991, 57: 169-173.［8］陈文杰, 梁江, 汤复跃, 等. 不同大豆种质种子耐浸水能力鉴定［J］. 中国油料作物学报, 2018,40(3): 381-390. (Chen W J, Liang J, Tang F Y, et al. Analysis of seed-flooding tolerance of soybean germplasms［J］. Chinese Journal of Oil Crop Sciences, 2018, 40(3): 381-390.)［9］陈文杰, 陈渊, 梁江, 等. 不同浸水时间对大豆种子发芽的影响［J］. 南方农业学报, 2013, 44(12): 1981-1984. (Chen W J, Chen Y, Liang J, et al. Effect of different soaking durations on soybean seed germination［J］. Journal of Southern Agriculture, 2013, 44(12):1981-1984.)［10］杨瑰丽, 杨美娜, 李帅良, 等. 水稻萌芽期抗旱指标筛选与抗旱性综合评价［J］. 华南农业大学学报, 2015, 36(2): 1-5. (Yang M L, Yang M N, Li S L, et al. Screening and comprehensive evaluation of drought resistance indices of rice at germination stage［J］. Journal of South China Agricultural University, 2015, 36(2): 1-5.)［11］〖JP2〗邹德堂, 郭微, 孙健, 等. 水稻不同基因型耐盐相关性状主成分分析及综合评价［J］. 东北农业大学学报, 2018, 49(8): 1-9. (Zou D T, Guo W, Sun J, et al. Principal component analysis and comprehensive evaluation of salt tolerance related traits in different rice (Oryza sativa L.) genotypes［J］. Journal of Northeast Agricultural University, 2018, 49(8): 1-9.)［12］龙文靖, 万年鑫, 辜涛，等. 玉米苗期耐低铁能力的综合评价及其预测［J］. 植物遗传资源学报, 2015, 16(4): 734-742. (Long W J, Wan N X, Gu T, et al. Comprehensive evaluation and forecast of low iron toerant ability in maize seeding stage［J］. Journal of Plant Genetic Resources, 2015, 16(4) : 734-742.)［13］张海艳, 赵海军. 不同品种玉米萌发期和苗期耐盐性综合评〖JP2〗价［J］. 玉米科学, 2016, 24(5), 61-67. (Zhang H Y, Zhao H J. Comprehensive evaluation of salt tolerance of different cornvarieties at the germination and seedling stages［J］. Journal of Maize Sciences, 2016, 24(5), 61-67.)［14］李龙, 毛新国, 王景一, 等. 小麦种质资源抗旱性鉴定评价［J］. 作物学报, 2018, 44(7): 988-999. (Li L, Mao X G, Wang J Y, et al. Drought tolerance evaluation of wheat germplasm resources［J］. Acta Agronomica Sinica, 2018, 44(7): 988-999.)［15］白志英, 李存东, 孙红春, 等. 小麦代换系抗旱生理指标的主成分分析及综合评价［J］. 中国农业科学, 2008, 41(12): 4264-4272. (Bai Z Y, Li C D, Sun H C, et al. Principal component analysis and comprehensive evaluation on physiological indices of drought resistance in wheat substitution［J］. Scientia Agricultura Sinica, 2008, 41(12): 4264-4272.)［16］孙东雷, 卞能飞, 陈志德, 等. 花生萌发期耐盐性综合评价及耐盐种质筛选［J］. 植物遗传资源学报, 植物遗传资源学报, 2017, 18(6): 1079-1087. (Sun D L, Bian N F, Chen Z D, et al. Comprehensive evaluation of salt tolerance and screening for salt tolerant accessions of peanut ( Arachis hypogaea L.) at germination stage［J］. Journal of Plant Genetic resources, 2017, 18(6): 1079-1087.)［17］于天一, 林建材, 孙学武, 等. 花生幼苗耐酸鉴定指标筛选及综合评价［J］. 中国油料作物学报, 2017, 39(4): 488-495. (Yu T Y, Lin J C, Sun X W, et al. Screen of acid resistance evaluation indicators and comprehensive evaluation in peanut seedlings of different genotypes［J］. Chinese Journal of Oil Crop Sciences, 2017, 39(4): 488 -495.)［18］国家技术监督局. GB/T 3543.4-1995.农作物种子检验规程:发芽试验［S］. 北京: 中国标准出版社, 1995. (State Technology Supervision Administration. GB/T 3543.4-1995.Rules for testing crop seeds: Germination tests［S］. Beijing: China Standards Publishing House, 1995.)［19］盖钧镒. 试验统计方法［ M］. 北京: 中国农业出版社, 2000. (Gai J Y. Test Statistics method［M］. Beijing: China Agriculture Publishing Press, 2000.)［20］周广生, 梅方竹, 周竹青, 等. 小麦不同品种耐湿性生理指标综合评价及其预测［J］. 中国农业科学, 2003, 36(11): 1378-1382. (Zhou G S, Mei F Z, Zhou Z Q, et al. Comprehensive evaluation and forecast on physiological indices of water logging resistance of different whet varieties［J］. Scientia Agricultrua Sinica, 2003, 36(11): 1378-1382.)［21］常汝镇, 孙建英. 中国大豆品种资源目录（续编一）［M］. 北京: 农业出版社, 1991. (Chang R Z, Sun J Y. Catalogue of soybean germplasm resources in China (Continuation 1)［M］. Beijing: Agriculture Publishing Press, 1991.)［22］邱丽娟, 常汝镇. 大豆种质资源描述规范和数据标准［M］. 北京: 中国农业出版社, 2006: 69-73. (Qiu L J, Chang R Z. Descriptors and data standard for soybean (Glycine spp.)［M］. Beijing: China Agriculture Press, 2006: 69-73.)［23］王芳, 赵团结, 盖钧镒. 大豆野生与栽培资源苗期耐淹性的鉴定、生态区特征和优异种质发掘［J］. 大豆科学, 2007, 26(6): 828-834. (Wang F, Zhao T J, Gai J Y. Evaluation, eco-region characterization and elite germpalsm identification of submergence tolerance at seedling stage in wild and cultivated soybeans［J］. Soybean Science, 2007, 26(6): 828-834.)［24］Bailey S J, Voesenek L A C J. Flooding stress: Acclimations and genetic diversity［J］. Annual Review of Plant Biology, 2008, 59(1): 313-319.［25］Kawano N, Ito O, Sakagami J I. Morphological and physiological responses of rice seedlings to complete submergence(flash flooding)［J］. Annals of Botany, 2009,103(2):161-169.［26］Nakajima T, Seino A, Nakamura T, et al. Does pre-germination flooding-tolerance soybean cultivar germinate better under hypoxia conditions［J］. Plant Production Science, 2015, 18(2): 146-153.［27］Tian X H, Nakamura T, Kokubun M. The role of seed structure and oxygen responsiveness in pre-germination flooding tolerance of soybean cultivars［J］. Plant Production Science, 2005, 8(2): 157-165.［28］Sayama T, Nakazaki T, Ishikava G, et al. QTL analysis of seed-flooding tolerance in soybean (Glycine max ［L.］ Merr.)［J］. Plant Science, 2009, 176(4): 514-521.［29］刘瑞君, 靖静, 李志, 等. 栽培× 野生大豆2个重组自交家系群体种子耐淹性遗传分析［J］. 大豆科学, 32(5): 581-586. (Liu R J, Jing J, Li Z, et al. Genetic analysis of seed germination characters under submergence stress in two recombinant inbred line populations of Glycine max×Glycine soja［J］.Soybean Science, 32(5): 581-586.)［30］Ali M J, Yu Z P, Xing G N, et al. Establishment of evaluation procedure for soybean seed-flooding tolerance and its application to screening for tolerant germplasm sources［J］. Legume Research, 2017, 41(133): 1-9.
相似文献/References:
[1]李泽宇,李肖白,陈井生,等.大豆品种（系）抗大豆胞囊线虫14号生理小种的抗性鉴定研究[J].大豆科学,2014,33(03):408.[doi:10.11861/j.issn.1000-9841.2014.03.0408]
LI Ze-yu,LI Xiao-bai,CHEN Jing-sheng,et al.Identification of Soybean Varieties for Resistance to Soybean Cyst Nematode Races 14[J].Soybean Science,2014,33(02):408.[doi:10.11861/j.issn.1000-9841.2014.03.0408]
[2]朱英波,史凤玉,李建英,等.抗大豆胞囊线虫病野生大豆种质资源的初步筛选[J].大豆科学,2011,30(06):959.[doi:10.11861/j.issn.1000-9841.2011.06.0959]
ZHU Ying-bo,SHI Feng-yu,LI Jian-ying,et al.Identification on Soybean Germplasm Resistant to Soybean Cyst Nematode from Glycine soja[J].Soybean Science,2011,30(02):959.[doi:10.11861/j.issn.1000-9841.2011.06.0959]
[3]钟开珍,梁江,韦清源,等.大豆种质倒伏性遗传及其与主要农艺性状的相关分析[J].大豆科学,2012,31(05):703.[doi:10.3969/j.issn.1000-9841.2012.05.003]
ZHONG Kai-zhen,LIANG Jiang,WEI Qing-yuan,et al.Heredity of Lodging and Its Correlation with Agronomic Traits in Soybean Germplasm[J].Soybean Science,2012,31(02):703.[doi:10.3969/j.issn.1000-9841.2012.05.003]
[4]张海军,王英,张艳,等.东北地区大豆种质资源脂肪和蛋白质含量分析[J].大豆科学,2011,30(02):215.[doi:10.11861/j.issn.1000-9841.2011.02.0215]
ZHANG Hai-jun,WANG Ying,ZHANG Yan,et al.Analysis of Oil and Protein Content of Soybean Germplasm Grown in Northeast China[J].Soybean Science,2011,30(02):215.[doi:10.11861/j.issn.1000-9841.2011.02.0215]
[5]曹广禄,赵 雪,王 强,等.大豆种质资源对胞囊线虫病1号、3号和4号生理小种的抗性鉴定[J].大豆科学,2014,33(04):563.[doi:10.11861/j.issn.1000-9841.2014.04.0563]
CAO Guang-lu,ZHAO Xue,WANG Qiang,et al.Resistance Evaluation of Soybean Germplasm to Races 1,3 and 4 of Soybean Cyst Nematode(Heterodera giycines)[J].Soybean Science,2014,33(02):563.[doi:10.11861/j.issn.1000-9841.2014.04.0563]
[6]杨文钰,雍太文,任万军,等.发展套作大豆，振兴大豆产业[J].大豆科学,2008,27(01):1.[doi:10.11861/j.issn.1000-9841.2008.01.0001]
YANG Wen-yu,YONG Tai-wen,REN Wan-jun,et al.Develop Relay planting Soybean，Revitalize Soybean Industry[J].Soybean Science,2008,27(02):1.[doi:10.11861/j.issn.1000-9841.2008.01.0001]
[7]胡国玉,张丽亚,黄志平,等.黄淮夏大豆种质资源农艺性状的评价[J].大豆科学,2008,27(02):215.[doi:10.11861/j.issn.1000-9841.2008.02.0215]
HU Guo-yu,ZHANG Li-ya,HUANG Zhi-ping,et al.Evaluation of Agronomic Charicaters in Summer Soybean Germplsam of Huanghuai Region[J].Soybean Science,2008,27(02):215.[doi:10.11861/j.issn.1000-9841.2008.02.0215]
[8]杨明亮,宋雯雯,康明,等.大豆凝集素含量测定方法的改进与种质资源分析[J].大豆科学,2008,27(02):310.[doi:10.11861/j.issn.1000-9841.2008.02.0310]
YANG Ming-liang,SONG Wen-wen,KANG Ming,et al.An Improved Method For Determining SBA Content and Screening for Soybean Germplasms[J].Soybean Science,2008,27(02):310.[doi:10.11861/j.issn.1000-9841.2008.02.0310]
[9]齐波,赵团结,盖钧镒.中国大豆种质资源耐铝毒性的变异特点及优选[J].大豆科学,2007,26(06):813.[doi:10.3969/j.issn.1000-9841.2007.06.003]
QI Bo,ZHAO Tuan-jie,GAI Jun-yi.CHARACTERIZATION OF VARIATION AND IDENTIFICATION OF ELITE ACCESSIONS OF ALUMINUM TOXIN TOLERANCE SOYBEAN GERMPLASM IN CHINA[J].Soybean Science,2007,26(02):813.[doi:10.3969/j.issn.1000-9841.2007.06.003]
[10]王芳,赵团结,盖钧镒.大豆野生与栽培资源苗期耐淹性的鉴定、生态区特征和优异种质发掘[J].大豆科学,2007,26(06):828.[doi:10.3969/j.issn.1000-9841.2007.06.005]
WANG Fang,ZHAO Tuan-jie,GAI Jun-yi.EVALUATION，ECO-REGION CHARACTERIZATION AND ELITE GERMPALSM IDENTIFICATION OF SUBMERGENCE TOLERANCE AT SEEDLING STAGE IN WILD AND CULTIVATED SOYBEANS[J].Soybean Science,2007,26(02):828.[doi:10.3969/j.issn.1000-9841.2007.06.005]
备注/Memo