[1]林志伟,李响,李可,等.硝酸镧对黄绿木霉及其抑制大豆核盘菌能力的影响[J].大豆科学,2019,38(01):84-89.[doi:10.11861/j.issn.1000-9841.2019.01.0084]
　LIN Zhi-wei,LI Xiang,LI Ke,et al.Effect of Lanthanum Nitrate on Trichoderma Aureoviride and Its Inhibition Ability on Sclerotinia Sclerotiorum[J].Soybean Science,2019,38(01):84-89.[doi:10.11861/j.issn.1000-9841.2019.01.0084]
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硝酸镧对黄绿木霉及其抑制大豆核盘菌能力的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第38卷 期数: 2019年01期 页码: 84-89 栏目: 出版日期: 2019-01-20
Title:
Effect of Lanthanum Nitrate on Trichoderma Aureoviride and Its Inhibition Ability on Sclerotinia Sclerotiorum
作者:
林志伟; 李响; 李可; 肖翠红; 孙冬梅
(黑龙江八一农垦大学 农学院，黑龙江 大庆 163319）
Author(s):
LIN Zhi-wei; LI Xiang; LI Ke; XIAO Cui-hong; SUN Dong-mei
(Agronomy College of Heilongjiang Bayi Agricultural University, Daqing 163319,China)
关键词:
硝酸镧; 黄绿木霉菌; 大豆菌核病核盘菌; 拮抗; 对峙培养
Keywords:
Lanthanum nitrate ［La(NO3)3］; Trichoderma aureoviride; Sclerotinia sclerotiorum; Inhibition; Confrontation culture
DOI:
10.11861/j.issn.1000-9841.2019.01.0084
文献标志码:
A
摘要:
为研究黄绿木霉菌（Trichoderma aureoviride）的拮抗性能与硝酸镧之间的相互关系，以大豆菌核病核盘菌(Sclerotinia sclerotiorum)为指示菌，采用固体培养与液体培养两种方式，检测添加不同浓度硝酸镧对黄绿木霉菌拮抗作用的影响。结果表明：硝酸镧含量为210 μg·mL-1对黄绿木霉菌菌丝生长出现抑制，达到1 000 μg·mL-1时可完全抑制黄绿木霉菌的生长，但低于180 μg·mL-1的硝酸镧浓度可刺激黄绿木霉菌的生长；硝酸镧浓度高于330 μg·mL-1对大豆菌核病核盘菌菌丝生长出现抑制，达到 1 100 μg·mL-1时可完全抑制大豆菌核病核盘菌的生长。一定范围内，硝酸镧的添加对黄绿木霉菌拮抗大豆菌核病核盘菌无影响，对峙培养抑菌率仍可达100%；发酵中添加硝酸镧后，粗提物抑菌能力不变，但拮抗带明显加强。
Abstract:
In order to study the relationship between Trichoderma aureoviride antagonistic properties with lanthanum nitrate, Sclerotinia sclerotiorum was taken as the indicator, using solid and liquid culture methods, the effects of different concentrations of lanthanum nitrate on T.aureoviride antagonistic ability were detected. The results showed that the inhibited contention of lanthanum nitrate on the mycelial growth of T.aureoviride was 210 μg·mL-1, and the completely inhibited concentration was 1 000 μg·mL-1, but the concentration below 180 μg·mL-1 could stimulate the growth of mycelium. The inhibited concentration of lanthanum nitrate on the mycelial growth of S. sclerotiorum was higher than 330 μg·mL-1, the completely inhibited concentration was 1 100 μg·mL-1. Within a certain range, the addition of lanthanum nitrate has no inhibition effect of T.aureoviride on S.sclerotiorum, the inhibition rate under confrontation culture was still up to 100%. After the addition of lanthanum nitrate in fermentation, the inhibition ability of crude extracts remained unchanged, but the antagonistic bands were obviously strengthened.

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备注/Memo

收稿日期：2018-08-11

基金项目：大庆市指导项目（zd-2017-63）；黑龙江农垦总局项目（HNK135-02-06-04）；寒区环境微生物与农业废弃物资源化利用重点实验室项目（201707）；黑龙江省大学生创新项目（201710223040）；黑龙江八一农垦大学创新项目（xc2017033）。
第一作者简介：林志伟（1970-），男，硕士，副教授，主要从事植物保护研究。E-mail: lzwsdm@sohu.com。

更新日期/Last Update: 2019-01-22