[1]刘玉兰,陈殿元,元明浩,等.盐胁迫对小粒大豆幼苗生长发育及光合特性的影响[J].大豆科学,2017,36(06):913-920.[doi:10.11861/j.issn.1000-9841.2017.06.0913]
　LIU Yu-lan,CHEN Dian-yuan,YUAN Ming-hao,et al.Effects of Salt Stress on Seedling Growth and Photosynthetic Characteristics of Glycine gracilis[J].Soybean Science,2017,36(06):913-920.[doi:10.11861/j.issn.1000-9841.2017.06.0913]
点击复制

盐胁迫对小粒大豆幼苗生长发育及光合特性的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年06期 页码: 913-920 栏目: 出版日期: 2017-11-20
Title:
Effects of Salt Stress on Seedling Growth and Photosynthetic Characteristics of Glycine gracilis
作者:
刘玉兰; 陈殿元; 元明浩; 段开怀; 李皖; 张国印; 孙堂玉
（吉林农业科技学院 植物科学学院，吉林 吉林 132101）
Author(s):
LIU Yu-lan;  CHEN Dian-yuan;  YUAN Ming-hao;  DUAN Kai-huai;  LI Wan;  ZHANG Guo-yin; SUN Tang-yu
(School of Plant Science, Jilin Agricultural Science and Technology University, Jilin 132101, China)
关键词:
小粒大豆; 盐胁迫; 生长; 光合特性
Keywords:
Glycine gracilis;  Salt stress;  Growth;  Photosynthetic characteristics
DOI:
10.11861/j.issn.1000-9841.2017.06.0913
文献标志码:
A
摘要:
本文研究了不同盐（NaCl∶Na2SO4=1∶1和NaHCO3∶Na2CO3=1∶1）胁迫对小粒大豆东农690植株生长发育及光合特性的影响。结果表明：低浓度的中性盐对小粒豆生长具有一定的促进作用，低浓度的碱性盐对小粒豆生长抑制作用不明显；较高浓度盐胁迫抑制小粒豆生长。在两种盐浓度高于60 mmol·L1胁迫条件下，植株的生长发育及光合特性大幅改变，与对照相比差异极显著。小粒豆株高、根长、根瘤菌数、单株叶面积、干鲜比、净光合速率（Pn）、气孔导度（Gs）、蒸腾速率（Tr）、气孔限制值（Ls）各值与两种盐胁迫均呈极显著负相关；叶绿素含量与中性盐胁迫呈显著负相关，与碱性盐胁迫呈极显著负相关；叶片外浸液相对电导率、胞间CO2浓度（Ci）与两种盐胁迫均呈极显著正相关。
Abstract:
This study examined the effects of various salt stresses (NaCl∶Na2SO4=1∶1和NaHCO3∶Na2CO3=1∶1) on the plant growth and photosynthetic characteristics of small soybean variety Dongnong 690.The result showed that the low concentration of neutral salt accelerated the growth of Glycine gracilis to a certain extent, while alkaline salt didn′t induce obvious resistance, the high concentration of salt stress restrained the growth of Glycine gracilis.Under the condition of two kinds salt concentration higher than 60 mmol·L-1stress, compared with the control, the growth and photosynthetic characteristic of plant changed significantly. Each value of root length, root nodule bacteria number, leaf area, fresh and dry ratio, net photosynthetic rate(Pn), stomatal conductance(Gs), transpiration rate(Tr), and stomatal limitation value(Ls) of small soybean were significantly negatively correlated with two kinds of salt stress. Chlorophyll content was negatively correlated with neutral salt stress and negatively correlated with alkaline salt stress. Leaf extracts relative conductivity and intercellular CO2 concentration (Ci) were significantly and positively correlated with two kinds of salt stress.

参考文献/References:

［1］杨劲松. 中国盐渍土研究的发展历程与展望［J］. 土壤学报, 2008, 45(5): 837-845.(Yang J S. Development and prospect of the research on salt-affected soils in China［J］. Acta Pedologia Snica, 2008, 45(5): 837-845.)

［2］李子芳, 王玉国, 孙守钧. 植物应对盐胁迫的生理机制［J］. 河北农业科学, 2008, 12(3):1-3. (Li Z F, Wang Y G, Sun S J. The physiological mechanism of plants responding to salt stress［J］. Journal of Hebei Agricultural Sciences, 2008, 12(3): 1-3.)
［3］Bethke P C, Drew M C. Stomatal and nonstonmatal components to inhibition of photosynthesis in leaves of Captcum annuum during progressive exposure to NaCl salinity［J］. Plant Physiology, 1992, 99: 219-226．
［4］Walker R R, Blackmore D H. Carbon dioxide as similition and folier ion concentration in leaves of ［Citrus sinensis(L) Osbeck］ trees irrigated with NaCl and Na2SO4［J］. Australian Journal of Plant Physiology, 1993, 20: 173-85.
［5］Munns R. Physiological processes limiting plant growth in neutral soils: Some dogmas and hypotheses［J］. Plant Cell and Environment,1993,16: 15-24．
［6］张丽辉, 赵骥民, 范亚红. 中性盐胁迫对高粱苗期光合特性的影响［J］. 江苏农业科学, 2012, 40(8):100-101. (Zhang L H, Zhao Y M, Fan Y H. The physiological mechanism of plants responding to salt stress［J］. Journal of Hebei Agricultural Sciences, 2012,40(8):100-101.)
［7］张丽华, 赵洪祥, 谭国波, 等.灌溉方式对大豆光合性状及土壤水分利用率的影响［J］. 大豆科学, 2012, 31(4): 613-616.( Zhang L H, Zhao H X, Tan G B, et al. Effects of irrigation modes on photosynthetic characters and water use efficiency of soybean［J］. Soybean Science, 2012, 31(4): 613-616.
［8］吉林省农科院. 中国大豆育种与栽培［M］. 北京: 农业出版社, 1987:173-180 (Jilin Provincial Academy of Agricultural Sciences Soybean Breeding and Cultivation in China［M］. Beijing: Agricultural Press,1987:173-180.)
［9］郑淑波, 赵洪锟, 刘晓冬, 等.不同小粒大豆品种豆芽特性比较［J］. 吉林农业科学, 2013,3 8(5): 81-85. (Zheng S B, Zhao H K, Liu X D, et al. Comparison on the sprout characteristics of different small seed soybean varieties［J］. Journal of Jilin Agricultural Sciences, 2013, 38(5): 81-85.)
［10］蔚荣海, 雷耔耘, 李羡宏, 等. 吉林省小粒大豆与大粒大豆某些品质性状的比较研究［J］.吉林农业大学学报, 1998, 20(3): 12-16. (Wei R H, Lei Z Y, Li X H, et al. Study on comparison in quality properties of small grain and biggrain soybean in Jilin province［J］. Journal of Jilin Agricultural University, 1998, 20(3): 12-16.)
［11］孙广玉, 邹琦, 程炳嵩. 小粒大豆光合特性的研究［J］. 中国农业科学, 1991, 24(2): 57-62. (Sun G Y, Zou Q, Cheng B S. Studies on photosynthetic characteristics of a small-seed soybean［J］. 1991, 24(2): 57-62.)
［12］季平, 张鹏, 徐克章, 等. 不同类型盐碱胁迫对大豆植株生长性状和产量的影响［J］. 大豆科学, 2013, 329(4): 477-481. (Ji P, Zhang P, Xu K Z, et al.Effects of salt and alkaline stress on plant growth traits and yield of soybean［J］. Soybean Science, 2013, 32(4): 477-481.)
［13］曹保华. 盐胁迫影响大豆光合作用的部分机制［D］. 杭州: 浙江大学, 2007.(Cao B H. Partial mechanism of photosynthetic suppression in soybean varieties under salt stress［D］. Hangzhou: Zhejiang University, 2007.
［14］邵桂花, 万超文, 李舒凡. 大豆萌发期耐盐生理初步研究［J］. 作物生理, 1994(6): 25-26. (Shao G H, Wan C W, Li S F. Preliminary study on salt tolerance of soybean germination period［J］. Crop Physiological,1994(6):25-26.)
［15］寇贺,曹敏建,那桂秋Na2CO3和NaCl对大豆种子萌发胁迫效应的比较研究［J］.种子2007, 26(12): 27-31. (Kou H, Cao M J, Na G Q. Comparative studies on reaction at germination stage of soybean under Na2CO3 and NaCl stresses［J］. Seed, 2007, 26(12): 27-31.)
［16］王俊杰, 吕志男, 吕世杰, 等.黄花苜蓿野生种群种子萌发期的耐盐性研究［C］.常州：中国草学会饲料生产委员会第15次饲草生产学术研讨会论文集, 2009. (Wang J J, Lyu Z N, Lyu S J, et al. Study on tolerance of sickle alfalfa wild populations in germinating period［C］. Changzhou: China Grass Association Feed Production Committee 15th Forage Production Academic Seminar Proceedings, 2009.
［17］郑世英,萧蓓蕾,金桂芳 NaCl 胁迫对野生大豆和栽培大豆叶绿素及光合特性的影响［J］. 大豆科学, 2013,32(4):486-489. (Zheng S Y,Su B L,Jin G F. Effect of NaCl stress on chlorophyll content and photosynthetic characteristics of Glycine soja and Glycine max.［J］. Soybean Science, 2013,32(4):486-489.
［18］盖玉红,牛陆,董宝池,等.不同浓度盐、碱胁迫对野生大豆光合特性和生理生化特性的影响［J］.江苏农业科学,2015,42(5):89-93. (Gai Y H,Niu L,Dong B C,et al. Effects of different concentrations of salt and alkali stress on photosynthesis and physiological and biochemical characteristics of wild soybean［J］. Journal of Jiangsu Agricultural Sciences, 2015,42(5):89-93.
［19］宁丽华,张大勇,刘 佳,等.盐胁迫下苗期栽培大豆生理响应及 Na+动态平衡关键基因的表达［J］. 中国农业科学,2016,49(24):4714-4725. (Ning L H,Zhang D Y,Liu J,et al. Effect of salt stress on physiological reponses and the expression of key genes involved in Na+ homeostasis of soybean seedlings［J］. Scientia Agricultura Sinica, 2016,49(24):4714-4725.
［20］张治安,陈展宇. 植物生理学实验技术［M］.长春:吉林大学出版社,2008. (Zhang Z A, Chen Z Y. Experimental technique of plant physiology［M］.Changchun: Jilin University Press, 2008)
［21］李文誉,李德明.盐碱及重金属对植物生长发育的影响［J］.北方园艺, 2010, 34(8): 221-224.(Li W Y, Li D M. Effects of salinization and heavy metals stress on plant growth and development［J］. Northern Horticulture,2010,8:221-224.)
［22］范国强,曲巧晓,李松林.泡桐愈伤组织再生植株的诱导与培养 ［J］.植物学通报，2002,19(1):92-97. (Fan G Q, Qu Q X, Li S L. Plant regeneration from leaf calli of paulownia plants［J］. Chinese Bulletin of Botany,2002,19(1): 92-97.)
［23］Negro S, Courtois B, Ahmadi N, et al. Recent updates on salinity stress in rice: From physiological to molecular responses［J］. Plant Sciences, 2011, 30: 329-377.
［24］刘贵河,郭郁频,任永霞,等.PEG 胁迫下 5 种牧草饲料作物种子萌发期的抗旱性研究［J］.种子,2013,32(11):15-19.(Liu G H,Guo Y P,Ren Y X,et al. Study on drought resistance of five kinds forage and crops during seed germination period under PEG stress［J］. Seed, 2013,32(11):15-19.)
［25］庄华蓉.单盐和混合盐胁迫对沙冬青种子萌发的影响［J］.节水灌溉,2015(12):15-20.(Zhuang H R.Effects of single and compound salt stress on stress on seed germination of Ammopiptanthus mongolicus［J］. Water Saving Irrigation,2015(12):15-20.)

相似文献/References:

[1]陈 昀,郑焕明,张 竞,等.以hemin为基础的物质联用缓解盐胁迫对大豆种子萌发的抑制[J].大豆科学,2013,32(05):640.[doi:10.11861/j.issn.1000-9841.2013.05.0640]
　CHEN Yun,ZHENG Huan-ming,ZHANG Jing,et al.Hemin-based Combinations of Exogenous Substances Alleviate the Inhibition of Soybean Seed Germination under Salt Stress[J].Soybean Science,2013,32(06):640.[doi:10.11861/j.issn.1000-9841.2013.05.0640]
[2]王丽燕.硅对野生大豆幼苗耐盐性的影响及其机制研究[J].大豆科学,2013,32(05):659.[doi:10.11861/j.issn.1000-9841.2013.05.0659]
　WANG Li-yan.Effects of Silicon on Salt Tolerance of Glycine soja Seedlings and Its Mechanism[J].Soybean Science,2013,32(06):659.[doi:10.11861/j.issn.1000-9841.2013.05.0659]
[3]郑世英,萧蓓蕾,金桂芳.NaCl胁迫对野生大豆和栽培大豆叶绿素及光合特性的影响[J].大豆科学,2013,32(04):486.[doi:10.11861/j.issn.1000-9841.2013.04.0486]
　ZHENG Shi-ying,XIAO Bei-lei,JIN Gui-fang.Effect of NaCl Stress on Chlorophyll Content and Photosynthetic Characteristics of Glycine soja and Glycine max[J].Soybean Science,2013,32(06):486.[doi:10.11861/j.issn.1000-9841.2013.04.0486]
[4]邢承华,郑寨生,张尚法,等.黑豆根尖边缘细胞对盐胁迫的防护效应[J].大豆科学,2012,31(03):416.[doi:10.3969/j.issn.1000-9841.2012.03.016]
　XING Cheng-hua,ZHENG Zai-sheng,ZHANG Shang-fa,et al.Protective Effect of Root Border Cells on Salt Stress in Black Soybean[J].Soybean Science,2012,31(06):416.[doi:10.3969/j.issn.1000-9841.2012.03.016]
[5]张乃群,刘彦娜,解艳华.盐胁迫对野生大豆幼苗生长的影响[J].大豆科学,2012,31(06):920.[doi:10.3969/j.issn.1000-9841.2012.06.013]
　ZHANG Nai-qun,LIU Yan-na,XIE Yan-hua.Effects of Salt Stress on Growth of Wild Soybean(Glycine soja)Seedlings[J].Soybean Science,2012,31(06):920.[doi:10.3969/j.issn.1000-9841.2012.06.013]
[6]张辉,张文会,苗秀莲,等.外源脱落酸对苗期野生大豆抗盐能力的影响[J].大豆科学,2009,28(05):828.[doi:10.11861/j.issn.1000-9841.2009.05.0828]
　ZHANG Hui,ZHANG Wen-hui,MIAO Xiu-lian,et al.Exogenous Abscisic Acid Strengthen Salt Tolerance of Glycine soja at Seedling Stage[J].Soybean Science,2009,28(06):828.[doi:10.11861/j.issn.1000-9841.2009.05.0828]
[7]张秀玲.不同盐分胁迫对野生大豆种子发芽的影响[J].大豆科学,2009,28(03):461.[doi:10.11861/j.issn.1000-9841.2009.03.0461]
　ZHANG Xiu-ling.Effect of Salinities Stress on Seed Germination of Glycine.Soja[J].Soybean Science,2009,28(06):461.[doi:10.11861/j.issn.1000-9841.2009.03.0461]
[8]奚广生,王艳玲.外源激素对野生大豆耐盐性的影响[J].大豆科学,2007,26(06):972.[doi:10.3969/j.issn.1000-9841.2007.06.034]
　XI Guang-sheng,WANG Yan-ling.EFFECT OF EXOGENOUS PHYTOHORMONES ON SALT TOLERANCE IN GLYCINE SOJA[J].Soybean Science,2007,26(06):972.[doi:10.3969/j.issn.1000-9841.2007.06.034]
[9]付畅,关旸,徐娜.盐胁迫对野生和栽培大豆中抗氧化酶活性的影响[J].大豆科学,2007,26(02):144.[doi:10.3969/j.issn.1000-9841.2007.02.005]
　FU Chang,GUAN Yang,XU Na.EFFECT OF SALT STRESS ON THE ACTIVITY OF ANTIOXIDATIVE ENZYMES OF GLYCINE SOJA AND GLYCINE MAX[J].Soybean Science,2007,26(06):144.[doi:10.3969/j.issn.1000-9841.2007.02.005]
[10]付畅,关旸.盐胁迫下栽培大豆黑农40不同器官中抗氧化酶活性的变化[J].大豆科学,2007,26(03):343.[doi:10.3969/j.issn.1000-9841.2007.03.011]
　FU Chang,GUAN Yang.CHANGS OF SALT STRESS ON THE ACTIVITY OF ANTIOXIDATIVE ENZYMES IN DIFFERENT ORGANS OF GLYCINE MAX HEINONG 40[J].Soybean Science,2007,26(06):343.[doi:10.3969/j.issn.1000-9841.2007.03.011]

备注/Memo

基金项目：吉林省财政厅—吉林省省级粮食生产发展专项资金（科研育种项目）；吉林农业科技学院作物遗传育种-校级重点学科（112012015001）。

第一作者简介：刘玉兰（1971-），女，硕士，高级实验师，主要从事作物栽培及良种繁育研究。E-mail: jllyl2006@163.com。
通讯作者：陈殿元（1963-），男，硕士，教授，主要从事作物栽培与育种研究。E-mail:JLcdy@sina.com。

更新日期/Last Update: 2018-01-28