[1]安琦,殷博,张介驰,等.外源类黄酮对大豆根瘤菌趋化性与结瘤效果的影响[J].大豆科学,2017,36(06):900-904.[doi:10.11861/j.issn.1000-9841.2017.06.0900]
　AN Qi,YIN Bo,ZHANG Jie-chi,et al.The Chemotaxis and Nodulation Effect of Soybean Rhizobium on Exogenous Flavonoids[J].Soybean Science,2017,36(06):900-904.[doi:10.11861/j.issn.1000-9841.2017.06.0900]
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外源类黄酮对大豆根瘤菌趋化性与结瘤效果的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年06期 页码: 900-904 栏目: 出版日期: 2017-11-20
Title:
The Chemotaxis and Nodulation Effect of Soybean Rhizobium on Exogenous Flavonoids
作者:
安琦1; 2; 殷博1; 2; 张介驰1; 2; 袁明3; 王连霞3; 原韬1; 2; 曹亚彬1; 2; 牛彦波1; 2
（1.黑龙江省科学院 微生物研究所，黑龙江 哈尔滨 150010； 2.黑龙江省科学院 高技术研究院，黑龙江 哈尔滨 150020； 3.黑龙江省农业科学院 齐齐哈尔分院，黑龙江 齐齐哈尔 161006）
Author(s):
AN Qi1; 2 ;  YIN Bo1; 2;  ZHANG Jie-chi1; 2;  YUAN Ming3;  WANG Lian-xia3;  YUAN Tao1; 2;  CAO Ya-bin1; 2;  NIU Yan-bo1; 2
（1.Institute of Microbiology，Heilongjiang Academy of Sciences，Harbin 150010，China； 2.Institute of Advanced Technology，Heilongjiang Academy of Sciences，Harbin 150020，China； 3.Qiqihar Branch Academy，Heilongjiang Academy of Agricultural Sciences，Qiqihar 161006，China）
关键词:
外源类黄酮; 根瘤菌; 趋化性; 结瘤
Keywords:
Exogenous flavonoids; Rhizobium; Chemotaxis; Nodulation
DOI:
10.11861/j.issn.1000-9841.2017.06.0900
文献标志码:
A
摘要:
以慢生型大豆根瘤菌（Bradyrhizobium japonicum）2-39为供试菌株，分别以不同浓度（5，10，15，20，25 μg·mL-1）的大豆黄素、4-甲基伞形酮、金雀异黄酮、芒柄黄花素为诱导物，利用毛细管培养法对趋化的根瘤菌进行定量测定，研究不同种类和不同浓度的外源类黄酮对大豆根瘤菌趋化性的影响。选取对根瘤菌趋化性影响显著的外源类黄酮与大豆嫩丰16进行盆栽试验，进一步探究外源类黄酮对大豆结瘤效果的影响。结果表明：4种外源性类黄酮中，大豆黄素和金雀异黄酮对根瘤菌的趋化作用显著强于其它试验组，其最适作用浓度为15 μg·mL-1。盆栽试验结果显示：外源类黄酮大豆黄素和金雀异黄酮同时加入能够有效促进大豆结瘤。
Abstract:
In the study, Bradyrhizobium japonicum 2-39 was used as test strain Exogenous flavonoids such as daidzein, 4-methylumbelliferone, genistein and formononetin with different concentration (5, 10, 15, 20, 25 μg·mL-1) were selected as the inducer, respectively Aimed to study the chemotaxis effect of Bradyrhizobium japonicum 2-39 to exogenous flavonoids of different kinds and concentrations, capillary culture method was utilized to quantify the number of rhizobia colonies. The exogenous flavonoids which have significant effect on chemotaxis of Bradyrhizobium japonicum 2-39 and soybean genotype NF-16 were selected in the pot experiment to further explore the nodulation effect of soybean. The results indicated that daidzein and genistein exert more significant effect on Bradyrhizobium japonicum 2-39 compared with other group. The optimum concentration of daidzein and genistein was 15 μg·mL-1.?The results of pot experiment showed that simultaneous addition of daidzein and genistein could effectively stimulate soybean nodulation.

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备注/Memo

基金项目：国家现代农业产业技术体系（CARS-004-CES04）；国家现代农业产业技术体系子课题（CARS-210301）；黑龙江省科学院学部委员指导专项（XB2015SW01）；哈尔滨市应用技术研究与开发资助项目（2016RQQXJ227）。

第一作者简介：安琦（1988-），女，硕士，助理研究员，主要从事微生物学研究。E-mail：anqi2011stu@126.com。
通讯作者：张介驰（1971-），男，硕士，研究员，主要从事农业微生物研究。E-mail:zjclt@sina.com。

更新日期/Last Update: 2018-01-27