YUAN Jian-qin,ZHAO Jiang-he,SHI Zong-yong,et al.The Detection of Transgenic Soybean GTS 40-3-2 Component in Animal Feed[J].Soybean Science,2016,35(02):295-300.[doi:10.11861/j.issn.1000-9841.2016.02.0295]
动物饲料中转基因抗草甘膦大豆GTS 40-3-2成分的检测
- Title:
- The Detection of Transgenic Soybean GTS 40-3-2 Component in Animal Feed
- 关键词:
- 动物饲料; 转基因大豆GTS 40-3-2; 定性PCR; 实时荧光定量PCR; 检测
- Keywords:
- Animal feed; Genetically modified soybean GTS 40-3-2; Qualitative PCR; Real-time fluorescence quantitative PCR; Detection
- 文献标志码:
- A
- 摘要:
- 转基因抗草甘膦除草剂大豆GTS 40-3-2作为目前在最多国家和地区批准种植的转基因大豆，除榨取大豆油外，剩余的豆粕等副产品被用作动物饲料。通过对山西省太谷周边11个动物养殖户的饲料进行转基因标识情况专项调查，采用定性和定量PCR技术对转基因抗草甘膦除草剂大豆GTS 40-3-2含有的CaMV35-S启动子、NOS终止子、Cp4-EPSPS及大豆内标Lectin基因进行检测。结果表明：11份饲料全部含有转基因抗草甘膦除草剂大豆GTS -40-3-2成分，且其含量各不相同，其中蛋鸡饲料GTS 40-3-2转化体的含量最低，但所有饲料均无转基因标识。试验为我国转基因饲料饲用安全性评价体系提供了试验数据支持，同时也对来源于转基因作物的原材料产品的合理应用以及通过转基因饲料喂养动物所产生的农畜产品的安全性评价具有重要的现实和理论意义。
- Abstract:
- Genetically modified (GM) crops are planted widely to promote the global food production,alleviate the problem to the environment due to the use of pesticides, reduce poverty and hunger, bring ongoing considerable interest for the global environment (especially in the developing countries and regions), economic and social benefits. However, biological security problems from GM crops, as of the date of birth is accompanied by, the debate has never stopped Glyphosate resistant transgenic soybean GTS 40-3-2 is currently approved planting as GM crops in most countries and regions.In addition to extracting soybean oil, soybean meal and other remanining by-products are also used as animal feed. In order to detecting genetically modified ingredients in feed, experiments were carried out on the detection glyphosate resistant soybean of 11 animal feeds around Taigu of Shanxi.Qualitative and quantitative PCR were used to detect GTS 40-3-2, which contians CaMV35Spromoter and NOS terminator, Cp4EPSPS gene and soybean standard Lectin-gene. The detection results showed that 11 of feeds all contained genetically modified resistance to glyphosate GTS 40-3-2 soybean, and the content of each were not identical in 11 of feeds GTS 40-3-2 transformants of the laying hens feed was the lowest. The study provided data support for our GM feed forage safety evaluation system and had important theoretical and realistic significance from the reasonable application of transgenic livestock products by feeding animals producing or GM sources of raw materials products.
参考文献/References:
［1］Greiner R, Konietzny U. Presence of genetically modified maize and soy in food products sold commercially in Brazil from 2000 to 2005 ［J］. Food Control, 2008, 19: 499-505.
备注/Memo
基金项目：国家“十二五”科技支撑计划子课题（2012BAD12B06-2）；山西省科技攻关项目（20140311025-3）；山西省自然科学基金项目（2013011028-2）；山西省高等学校教学改革项目（J2012026）。