[1]王宇,钱华,姜欣欣,等.大豆食心虫非典型气味受体基因克隆及发育期表达分析[J].大豆科学,2016,35(01):124-129.[doi:10.11861/j.issn.1000-9841.2016.01.0124]
　WANG Yu,QIAN Hua,JIANG Xin-xin,et al.Cloning and Developmental Stages Expression of the Olfactory Coreceptor Gene in Leguminivora glycinivorella (Lepidoptera: Olethreutidae)[J].Soybean Science,2016,35(01):124-129.[doi:10.11861/j.issn.1000-9841.2016.01.0124]
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大豆食心虫非典型气味受体基因克隆及发育期表达分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年01期 页码: 124-129 栏目: 出版日期: 2016-01-20
Title:
Cloning and Developmental Stages Expression of the Olfactory Coreceptor Gene in Leguminivora glycinivorella (Lepidoptera: Olethreutidae)
作者:
王宇1; 2; 钱华1; 姜欣欣3; 张玉磊1; 苏戈4; 韩岚岚2; 赵奎军2
1.黑龙江省农业科学院 农化研究所，黑龙江 哈尔滨 150086；
2.东北农业大学 农学院，黑龙江 哈尔滨 150030；
3.东北农业大学 水利与建筑学院，黑龙江 哈尔滨 150030；
4.黑龙江农业科学院 信息中心，黑龙江 哈尔滨 150086
Author(s):
WANG Yu1; 2;  QIAN Hua1;  JIANG Xin-xin3;  ZHANG Yu-lei1;  SU Ge4;  HAN Lan-lan2;  ZHAO Kui-jun2
1.Agricultural Chemistry Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China;?
2.Agronomy College of Northeast Agricultural University, Harbin 150030, China;?
3.Water Conservancy and Civil Engineeing College of Northeast Agricultural University, Harbin 150030, China;?
4.Information Center of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
关键词:
大豆食心虫; 嗅觉受体; 基因克隆; 序列分析; 发育期表达量
Keywords:
Leguminivora glycinivorella;  Olfactory receptor;  Gene clone;  Sequence analysis;  Expression quantity of developmental stages
DOI:
10.11861/j.issn.1000-9841.2016.01.0124
文献标志码:
A
摘要:
非典型气味受体是昆虫嗅觉识别过程中重要的蛋白之一。本研究利用RT-PCR和巢式PCR的方法克隆了大豆食心虫非典型气味受体Olfactory co-receptor基因，命名为LglyOrco，其编码区为1 419 bp，编码473个氨基酸，氨基酸序列中存在3个N位糖基化位点，分子量为53.5 kD，等电点为7.12，含7个跨膜结构以及4个位于细胞膜外的亲水区，符合气味受体的典型结构特征。与鳞翅目已知昆虫的Orco同源性达到89%以上。不同发育期LglyOrco表达量分析表明，LglyOrco在二龄和三龄幼虫中的表达量是一龄幼虫的4~5倍，在四龄和五龄幼虫中的表达量是一龄幼虫的70%和50%，而在雌性成虫中的表达量是雄性成虫中的11倍。本研究将为大豆食心虫的嗅觉识别系统和大豆食心虫防治技术研究提供理论依据。
Abstract:
Olfactory co-receptor is one of the most important proteins in the process of insect identify system.In this study we cloned and analyzed an olfactory coreceptor of L. glycinivorella, which was named LglyOrco.The results showed that the full-length open reading frame of LglyOrco was 1 419 bp, encoding 473 amino acid residues and 3 N.glycosylation sites.The molecular weight of LglyOrco was 53.5 kDa and isoelectric point was 7.12. Blast searching in NCBI showed that the deduced amino acid sequence was highly similar with that of other .Orcos of Lepidoptera with acid sequence identities above 89%.LglyOrco- expression in second and third instar larva were 3 to 4 times higher than the first instar larva, forth and fifth instars larva were only 70% and 50% of the first instar larva respectively. The expression of LglyOrco was 10 times higher in the female adult than in the male adult. This study provides theoretical basis to the researching of L. glycinivorella identify system and control technique of L. glycinivorella.

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备注/Memo

基金项目：黑龙江省农业科技创新工程（2012ZD035）。

第一作者简介：王宇（1981-），男，博士，主要从事有害生物防治和综合治理研究。E-mail:wangyuryan@163.com。
通讯作者：赵奎军（1960-），男，博士，教授，主要从事害虫生物防治与综合治理研究。E-mail：kjzhao@163.com。

更新日期/Last Update: 2016-02-05