LI Jiao-jiao,XU Zhou,LIANG Cui-yue,et al.Effects of Nodulation on Soybean Growth, Malate Synthesis and Exudation in Different Phosphorus Concentrations[J].Soybean Science,2015,34(04):643-647.[doi:10.11861/j.issn.1000-9841.2015.04.0643]
不同磷浓度下结瘤对大豆生长及苹果酸合成和分泌的影响
- Title:
- Effects of Nodulation on Soybean Growth, Malate Synthesis and Exudation in Different Phosphorus Concentrations
- Keywords:
- Phosphorus availability; Rhizobium; Soybean; Malate
- 文献标志码:
- A
- 摘要:
- 以大豆品种粤春03-3为研究对象，采用水培的方式，在高低磷条件下，分别进行接种或不接种根瘤菌的处理。在大豆花期和成熟期分别测定大豆干重、氮磷浓度、根系苹果酸浓度和分泌速率，以研究结瘤对磷调控大豆生长及苹果酸合成和分泌的影响。结果表明：在低磷条件下，结瘤对大豆植株的干重、氮磷浓度均没有显著影响；在高磷条件下，结瘤能够显著提高植株地上部干重和氮浓度。在不结瘤条件下，磷对根系苹果酸浓度没有显著影响。但结瘤后，低磷明显增加了成熟期根系苹果酸的浓度。而且，结瘤显著提高了高磷条件下根系苹果酸的分泌速率。这些结果表明，结瘤影响了磷对大豆生长、根系苹果酸合成和分泌的调控。
- Abstract:
- The objective of study was to evaluate the effects of nodulation on the response of malate synthesis and exudation from soybean roots to phosphorus(P) availability. One soybean genotype YC03-3 was used, and inoculated with or without rhizobia under both high and low P conditions. At both flowering and maturing stages, plant dry weight, nitrogen(N) and P concentration, malate concentration and exudation were measured. The results showed that nodulated plants had higher biomass and N/P concentration than those in non-nodualted plants under high P conditions.However, no significant difference was observed under low P conditions.Malate concentration in non-nodulated roots was not influenced by P availability.However, malate concentration in nodulated roots was significantly higher at low P level than that at high P level at maturing stage. Moreover, malate exudation was significantly higher in the nodulated plants than that in non-nodulated plants under high P conditions. The results indicated that nodulation was involved in regulation of phosphate starvation responsive soybean growth, malate concentration and exudation.
参考文献/References:
［1］卢仁骏,严小龙,黄志武,等. 广东省砖红壤旱地土壤养分状况的网室调查［J］. 华南农业大学学报, 1992(2):74-80. (Lu R J, Yan X L, Huang Z W, et al.Greenhouse survey studies on upland latosol soil nutrient status in Guangdong province［J］.Journal of South China Agricultural University, 1992(2):74-80)［2］Tian J, Wang X, Tong Y, et al. Bioengineering and management for efficient phosphorus utilization in crops and pastures［J］. Current Opinion in Biotechnology, 2012, 23(6):866-871［3］Israel D W. Investigation of the role of phosphorus in symbiotic dinitrogen fixation［J］. Plant Physiology, 1987, 84(3):835-840［4］Israel D W.Symbiotic dinitrogen fixation and host-plant growth during development of and recovery from phosphorus deficiency［J］. Physiologia Plantarum, 1993, 88(2):294-300［5］Drevon J, Hartwig U A. Phosphorus deficiency increases the argon-induced decline of nodule nitrogenase activity in soybean and alfalfa［J］.Planta, 1997, 201(4):463-469［6］Almeida J F, Hartwig U A, Frehner M, et al. Evidence that P deficiency induces N feedback regulation of symbiotic N2 fixation in white clover (Trifolium repens L)［J］.Journal of Experimental Botany, 2000, 51(348):1289-1297［7］Hellsten A, HussDanell K. Interaction effects of nitrogen and phosphorus on nodulation in red clover (Trifolium pratense L)［J］. Acta Agriculturae Scandinavica, Section B.Plant Soil Science, 2000, 50(3):135-142［8］Jakobsen I. The role of phosphorus in nitrogen fixation by young pea plants (Pisum sativum)［J］. Physiologia Plantarum, 1985, 64(2):190-196［9］Qin L, Zhao J, Tian J, et al.The highaffinity phosphate transporter GmPT5 regulates phosphate transport to nodules and nodulation in soybean［J］. Plant Physiology, 2012, 159(4):1634-1643［10］Qin L, Jiang H, Tian J, et al.Rhizobia enhance acquisition of phosphorus from different sources by soybean plants［J］. Plant and Soil, 2011, 349(1-2):25-36［11］Yan X, Liao H, Trull M C, et al.Induction of a major leaf acid phosphatase does not confer adaptation to low phosphorus availability in common bean［J］. Plant Physiology, 2001, 125(4):1901-1911［12］Lynch J, Epstein E, L.uchli A, et al. An automated greenhouse sand culture system suitable for studies of P nutrition［J］. Plant, Cell & Environment, 1990, 13(6):547-554［13］马剑，黄高宝，高亚琴，等. 接种根瘤菌对豌豆根际细菌数量动态变化及产量的影响［J］.干旱地区农业研究, 2009, 27(6):7-10.(Ma J, Huang G B, Gao Y Q, et al. Effect of inoculated peas with rhizobium on the number of pea rhizobacteria dynamic changes and yields［J］. Agricultural Research in the Arid Areas, 2009, 27(6):7-10)［14］苗淑杰，乔云发，韩晓增，等.缺磷对已结瘤大豆生长和固氮功能的影响［J］. 作物学报, 2009, 35(7):1344-1349 (Miao S J, Qiao Y F, Han X Z, et al.Effects of phosphorus deficiency on growth and nitrogen fixation of soybean after nodule formation［J］. Acta Agronomica Sinica, 2009, 35(7):1344-1349)［15］Chen Z, Cui Q, Liang C, et al. Identification of differentially expressed proteins in soybean nodules under phosphorus deficiency through proteomic analysis［J］. Proteomics, 2011, 11(24):4648-.4659［16］Sa T, Israel D W. Energy status and functioning of phosphorusdeficient soybean nodules［J］. Plant Physiology, 1991, 97(3):928-935［17］Le Roux M R, Khan S, Valentine A J. Organic acid accumulation may inhibit N2 fixation in phosphorusstressed lupin nodules［J］. New Phytologist, 2008, 177(4):956-964［18］Schulze J, Temple G, Temple S J, et al.Nitrogen fixation by white lupin under phosphorus deficiency［J］. Annals of Botany, 2006, 98(4):731-740(上接第647页)［19］吴明才，肖昌珍，郑普英. 大豆磷素营养研究［J］.中国农业科学, 1999, 32(3):59-65. (Wu M C, Xiao C Z, Zheng P Y. Study on the physiological function of phosphorus to soybean［J］. Scientia Agricultura Sinica, 1999, 32(3):59-65)［20］丁娇. 长期施肥对大豆固氮能力的影响［D］.哈尔滨: 东北农业大学, 2013: 8-10 (Ding J.Effect of longterm fertilization on nitrogen fixation ability of soybean［D］ Harbin: Northeast Agricultural University, 2013: 8-10)［21］姚玉波. 大豆根瘤固氮特性与影响因素的研究［D］.哈尔滨: 东北农业大学, 2012: 48-56.(Yao Y B. Study on characteristics of nodule nitrogen fixation and influencing factors of soybean［D］. Harbin: Northeast Agricultural University, 2012: 48-56)［22］Israel D W.Investigation of the role of phosphorus in symbiotic dinitrogen fixation［J］. Plant Physiology, 1987, 84(3):835-840［23］Gahoonia T S, Nielsen N E, Lyshede O B. Phosphorus (P) acquisition of cereal cultivars in the field at three levels of P fertilization［J］. Plant and Soil, 1999, 211(2):269-281［24］兰忠明，林新坚，张伟光，等. 缺磷对紫云英根系分泌物产生及难溶性磷活化的影响［J］.中国农业科学, 2012, 45(8):1521-1531.(Lan Z M, Lin X J, Zhang W G, et al. Effect of P deficiency on the emergence of Astragalus L.root exudates and mobilization of sparingly soluble phosphorus ［J］. Scientia Agricultura Sinica, 2012, 45(8):1521-1531)［25］张振海，陈琰，韩胜芳，等. 低磷胁迫对大豆根系生长特性及分泌 H+和有机酸的影响［J］.中国油料作物学报, 2011, 33(2):135-140. (Zhang Z H, Chen Y, Han S F, et al. Effect of P deficiency stress on soybean root system and its secretion of H+ and organic acid ［J］. Chinese Journal of Oil Crop Sciences, 2011, 33(2):135-140)［26］Branscheid A, Sieh D, Pant B D, et al.Expression pattern suggests a role of MiR399 in the regulation of the cellular response to local Pi increase during -arbuscular mycorrhizal symbiosis ［J］. Molecular PlantMicrobe Interactions, 2010, 23: 915-926.
相似文献/References:
[1]王宏光,孙殿君,马忠强,等.大豆根瘤菌HD001的分离鉴定及结瘤能力检测[J].大豆科学,2014,33(03):379.[doi:10.11861/j.issn.1000-9841.2014.03.0379]
WANG Hong-guang,SUN Dian-jun,MA Zhong-qiang,et al.Isolation and Identification of Rhizobium HD001 and Its Nodulation Capacity Test in Soybean Germplasm[J].Soybean Science,2014,33(04):379.[doi:10.11861/j.issn.1000-9841.2014.03.0379]
[2]谢甫绨,孙海姝,张惠君,等.磷素对不同品质类型大豆光合生理的影响[J].大豆科学,2012,31(02):232.[doi:10.3969/j.issn.1000-9841.2012.02.014]
XIE Fu-ti,SUN Hai-shu,ZHANG Hui-jun,et al.Effect of P2O5 on Photosynthetic Physiology of Soybean Cultivars with Different Quality Types[J].Soybean Science,2012,31(04):232.[doi:10.3969/j.issn.1000-9841.2012.02.014]
[3]裴晓峰,关大伟,李 俊,等.耐旱大豆根瘤菌的筛选及其接种效应[J].大豆科学,2012,31(03):420.[doi:10.3969/j.issn.1000-9841.2012.03.017]
PEI Xiao-feng,GUAN Da-wei,LI Jun,et al.Screening of Drought-Tolerance Rhizobium and Its Influence on Soybean[J].Soybean Science,2012,31(04):420.[doi:10.3969/j.issn.1000-9841.2012.03.017]
[4]孟庆英,张春峰,于忠和,等.根瘤菌对大豆根际土壤微生物及大豆农艺性状的影响[J].大豆科学,2012,31(03):498.[doi:10.3969/j.issn.1000-9841.2012.03.035]
MENG Qing-ying,ZHANG Chun-feng,YU Zhong-he,et al.Effects of Rhizobia on Rhizosphere Soil Microoganisms and Agronomic Characters of Soybean[J].Soybean Science,2012,31(04):498.[doi:10.3969/j.issn.1000-9841.2012.03.035]
[5]吴萍,何庆元,李正鹏,等.安徽省大豆根瘤菌表型多样性研究[J].大豆科学,2011,30(02):219.[doi:10.11861/j.issn.1000-9841.2011.02.0219]
WU Ping,HE Qing-yuan,LI Zheng-peng,et al.Phenotypic Diversity of Soybean Rhizobia in Anhui Province[J].Soybean Science,2011,30(04):219.[doi:10.11861/j.issn.1000-9841.2011.02.0219]
[6]杨升辉,王素阁,于会勇,等.接种根瘤菌对夏大豆籽粒灌浆特性及品质的影响[J].大豆科学,2014,33(04):534.[doi:10.11861/j.issn.1000-9841.2014.04.0534]
YANG Sheng-hui,WANG Su-ge,YU Hui-yong,et al.Effects of Rhizobial Inoculation on the Grain Filling Characteristics and Quality of Summer Soybean[J].Soybean Science,2014,33(04):534.[doi:10.11861/j.issn.1000-9841.2014.04.0534]
[7]李馨园,王守义,王淑荣,等.根瘤菌配施胶质类芽孢杆菌对大豆叶绿素荧光特性、产量及品质的影响[J].大豆科学,2014,33(04):541.[doi:10.11861/j.issn.1000-9841.2014.04.0541]
LI Xin-yuan,WANG Shou-yi,WANG Shu-rong,et al.Effect of Rhizobium Combined with Pamibacillus mucilaginosus on Soybean Chlorophyll Fluorescence Characteristics,Yield and Quality[J].Soybean Science,2014,33(04):541.[doi:10.11861/j.issn.1000-9841.2014.04.0541]
[8]王媛媛,段玉玺,陈立杰,等.拮抗性大豆根瘤菌原生质体制备研究[J].大豆科学,2010,29(01):92.[doi:10.11861/j.issn.1000-9841.2010.01.0092]
WANG Yuan-yuan,DUAN Yu-xi,CHEN Li-jie,et al.Protoplast Formation of Antagonistic Rhizobium[J].Soybean Science,2010,29(04):92.[doi:10.11861/j.issn.1000-9841.2010.01.0092]
[9]尹丽娜,段玉玺,王媛媛,等.拮抗大豆胞囊线虫根瘤菌的研究[J].大豆科学,2010,29(02):276.[doi:10.11861/j.issn.1000-9841.2010.02.0276]
YIN Li-na,DUAN Yu-xi,WANG Yuan-yuan,et al.Screening of Rhizobia Against Soybean Cyst Nematode[J].Soybean Science,2010,29(04):276.[doi:10.11861/j.issn.1000-9841.2010.02.0276]
[10]李涛,关大伟,李俊,等.黄淮海地区优良大豆根瘤菌株的筛选与接种方式研究[J].大豆科学,2010,29(04):645.[doi:10.11861/j.issn.1000-9841.2010.04.0645]
LI Tao,GUAN Da-wei,LI Jun,et al.Screening of Superior Soybean Rhizobial Strains and Approach to Inoculation Methods for Region of HuangHuaiHai[J].Soybean Science,2010,29(04):645.[doi:10.11861/j.issn.1000-9841.2010.04.0645]
[11]苗淑杰,乔云发,韩晓增.磷和根瘤菌交互作用对大豆结瘤和生长的影响[J].大豆科学,2009,28(02):271.[doi:10.11861/j.issn.1000-9841.2009.02.0271]
MIAO Shu-jie,QIAO Yun-fa,HAN Xiao-zeng.Effect of Phosphorus and Rhizobium on Growth and Nodule Development in Soybean[J].Soybean Science,2009,28(04):271.[doi:10.11861/j.issn.1000-9841.2009.02.0271]
备注/Memo
基金项目：国家重点基础研究发展计划“973计划”(2011CB100301)；国家自然科学基金(31301835)。