[1]郭婕,胡利宗,李俐俐,等.黑豆豆芽中大豆异黄酮微波提取工艺研究[J].大豆科学,2015,34(03):493-496.[doi:10.11861/j.issn.1000-9841.2015.03.0485]
　GUO Jie,HU Li-zong,LI Li-li,et al.Extraction Technology of Soybean Isoflavones from Black Soybean Sprouts Using Microwave-assisted Extraction[J].Soybean Science,2015,34(03):493-496.[doi:10.11861/j.issn.1000-9841.2015.03.0485]
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黑豆豆芽中大豆异黄酮微波提取工艺研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年03期 页码: 493-496 栏目: 出版日期: 2015-06-25
Title:
Extraction Technology of Soybean Isoflavones from Black Soybean Sprouts Using Microwave-assisted Extraction
作者:
郭婕; 胡利宗; 李俐俐; 盛东峰
周口师范学院 生命科学与农学学院，河南 周口 466001
Author(s):
GUO Jie;  HU Li-zong;  LI Li-li;  SHENG Dong-feng
College of Life Science and Agronomy,Zhoukou Normal University, Zhoukou 466001, China
关键词:
黑豆; 豆芽; 大豆异黄酮; 微波提取
Keywords:
Black soybean;  Soybean sprouts;  Soybean isoflavones;  Microwave-assisted extraction
DOI:
10.11861/j.issn.1000-9841.2015.03.0485
文献标志码:
A
摘要:
以黑豆为原料，按照一定条件萌发处理。利用微波辅助提取法，通过对料液比、乙醇浓度、微波处理时间、微波功率进行单因素和正交试验，探讨黑豆豆芽中大豆异黄酮提取率的影响因素，并优化筛选其最佳提取工艺条件。结果表明：影响大豆异黄酮提取率的因素顺序为：微波处理时间＞料液比＞微波功率＞乙醇浓度。黑豆豆芽中大豆异黄酮的最佳微波提取工艺为：料液比1∶25、乙醇浓度50%、微波处理4 min、微波功率为450 W。在此条件下，黑豆豆芽中大豆异黄酮的提取率为0.595%。
Abstract:
Black soybeans was pre-treated under certain conditions being soaked at 30℃ for 15 h and germination at 25℃ for 72 h.The influence factors impacting extraction yield of soybean isoflavones from black soybean sprouts were explored, and the optimal extraction conditions using microwave-assisted extraction were optimized by single factor and orthogonal experiment, which included solid-liquid ratio, ethanol concentration, microwave-heating time and microwave power.The results showed that the order of different influence factors on yield was microwave-heating time ＞ solid-liquid ratio ＞ microwave power ＞ ethanol concentration.The optimal conditions were as follows: the 1∶25 ratio of solid-liquid, 50% ethanol, extracting for 4 min, microwave power was 450 W.The yield of soybean isoflavones was up to 0.595% under those conditions.

参考文献/References:

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备注/Memo

基金项目：周口师范学院大学生科研创新基金项目（ZKNUDXS2014-021）；河南省高等学校重点科研项目（15A180065）。

第一作者简介：郭婕（1984-），女，硕士，讲师，主要从事天然药物化学的研究与开发。E-mail: guojie.1023@163.com。

更新日期/Last Update: 2015-07-16