[1]李烨彤,苏志光,张惠,等.糖基化大豆蛋白酶解物的分离纯化及耐消化稳定性的研究[J].大豆科学,2015,34(03):474-479.[doi:10.11861/j.issn.1000-9841.2015.03.0474]
　LI Ye-tong,SU Zhi-guang,ZHANG Hui,et al.Separation and Purification of Glyco-soyprotein Hydrolysis and Its Digestion’s Resistance Stability[J].Soybean Science,2015,34(03):474-479.[doi:10.11861/j.issn.1000-9841.2015.03.0474]
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糖基化大豆蛋白酶解物的分离纯化及耐消化稳定性的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年03期 页码: 474-479 栏目: 出版日期: 2015-06-25
Title:
Separation and Purification of Glyco-soyprotein Hydrolysis and Its Digestion’s Resistance Stability
作者:
李烨彤; 苏志光; 张惠; 郭顺堂
中国农业大学 食品科学与营养工程学院，北京 100083
Author(s):
LI Ye-tong;  SU Zhi-guang;  ZHANG Hui;  GUO Shun-tang
College of Food Science and Nutritional Engineering，China Agricultural University，Beijing 100083，China
关键词:
糖基化; 大豆蛋白; 酶解物; 消化
Keywords:
Glycosylation;  Soyprotein;  Hydrolysates;  Digestion
DOI:
10.11861/j.issn.1000-9841.2015.03.0474
文献标志码:
A
摘要:
以糖基化大豆蛋白为原料，经碱性蛋白酶酶解制得具有抑制致病菌粘附功能的糖肽，并分析功能性糖肽的消化稳定性。酶解物用Sephadex G-25层析可获得表观分子量分别为20 000-Da、6 500 Da和小于3 000 Da组分。对酶解物胃蛋白酶和胰蛋白酶体外模拟依次消化2 h后， 20 000～6 500 Da的大豆糖肽分子量变化不大，较稳定，而分子量小于3 000 Da的大豆糖肽中有5%被消化，稳定性略有变化。说明糖基化大豆蛋白酶解物其功能性成分可以较稳定存在于消化系统。
Abstract:
Using glycol-soyprotein as raw material, enzymolysed by alkaline protease to obtain glycopeptides with th function of inhibiting pathogenic bacteria adhesion, the digestion of glycopeptide stability was analyzed.Enzymolysed content with Sephadex G-25 chromatography could obtain molecular weight of 20 000 Da, 6 500 Da and less than3 000 Da components. After enzymolysed by Pepsin and trypsin in vitro 2 hours in sequence, glycopeptides molecular weight between 20 000 Da and 6 500 Da were relatively stable, but the molecular weight less than 3 000 Da had been digested 5%.It indicated that glycopeptides with functional ingredients could exist in the digestive system steadily.

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备注/Memo

基金项目：国家“十二五”科技支撑计划（2012BAD34B04）。

第一作者简介：李烨彤(1990-)， 女，硕士，主要从事蛋白质加工研究。E-mail: yetong091@163.com。
?通讯作者：郭顺堂（1962-），男，教授，博导，主要从事蛋白质加工利用。E-mail: shuntang@cau.edu.cn。

更新日期/Last Update: 2015-07-16