[1]宋洁,许艳丽,姚钦.昆虫病原线虫共生细菌对植物寄生线虫卵的毒性研究[J].大豆科学,2014,33(06):896-899.[doi:10.11861/j.issn.1000-9841.2014.06.0896]
　SONG Jie,XU Yan-li,YAO Qin.Toxicity of Entomopathogenic Nematode Symbiotic Bacteria Strains on Eggs of Plant Parasite Nematodes[J].Soybean Science,2014,33(06):896-899.[doi:10.11861/j.issn.1000-9841.2014.06.0896]
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昆虫病原线虫共生细菌对植物寄生线虫卵的毒性研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年06期 页码: 896-899 栏目: 出版日期: 2014-12-25
Title:
Toxicity of Entomopathogenic Nematode Symbiotic Bacteria Strains on Eggs of Plant Parasite Nematodes
文章编号:
1000-98412014.06.0896
作者:
宋洁1; 2; 许艳丽1; 姚钦1
1.中国科学院 东北地理与农业生态研究所/黑土区农业生态院重点实验室，黑龙江 哈尔滨150081；
2.东北农业大学 农学院，黑龙江 哈尔滨 150030
Author(s):
SONG Jie1; 2; XU Yan-li1; YAO Qin1
1.Key Laboratory of Mollisols Agroecology,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Harbin 150081,China;
2.College of Agriculture,Northeast Agricultural University,Harbin 150030,China
关键词:
昆虫病原线虫; 共生细菌; 大豆胞囊线虫; 南方根结线虫; 卵孵化; 毒性
Keywords:
Entomopathogenic; Symbiotic bacteria; Heterodera glycines; Meloidogyne incognita; Egg hatching; Toxicity
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.06.0896
文献标志码:
A
摘要:
为探讨昆虫病原线虫体内携带的共生细菌对植物寄生线虫卵的毒性，从实验室保存的4个品系昆虫病原线虫中分离出4个共生细菌菌株，分别为Photorhabdus luminescens-NJ、Photorhabdus luminescens-Hb（USA）、Xenorhabdus nematophilus-Sc和Xenorhabdus nematophilus-Sc-All，检测了共生细菌对植物寄生线虫卵孵化的影响。结果表明：4个品系共生细菌对大豆胞囊线虫和根结线虫的卵孵化均表现出了明显的抑制作用，对大豆胞囊线虫和根结线虫卵孵化抑制率范围分别为74.1%～100%和57.8%～89.2%，但是不同品系所表现出的抑制作用不同，光杆菌属（Photorhabdus spp.）NJ菌株和Hb（USA）菌株对大豆胞囊线虫卵孵化的毒性明显强于致病杆菌属（Xenorhabdus spp.）Sc和ScAll菌株，卵孵化抑制率分别为100%和89.7%，而对根结线虫卵孵化的毒性致病杆菌属（Xenorhabdus spp.）SC菌株和SC-All则表现出较好的效果，卵孵化抑制率分别达到89.2%和78.2%。证明了昆虫病原线虫共生细菌对植物寄生线虫卵具有毒性。
Abstract:
Toxicity of entomopathogenic nematodes with symbiotic bacteria on egg hatching of the plant parasitic nematodes was examined.Four strains of entomopathogenic nematode symbiotic bacteria were isolated from different entomopathogenic nematodes including Photorhabdus luminescens-NJ,Photorhabdus luminescens-Hb（USA）,Xenorhabdus nematophilus-Sc and Xenorhabdus nematophilus-Sc-All.The effect of the four strains on the egg hatching of plant parasitic nematodes was investigated.The result indicated that the four strains significantly inhibited egg hatching of these plant parasitic nematodes.The inhibition range was from 74.1% to 100% with soybean cyst nematodes and from 57.8% to 89.2% with Meloidogyne incognita.But suppressive effects were variable with different strains.The suppression of NJ and Hb（USA）strains were better than Sc and ScAll strains on soybean cyst nematode,and the percentage of inhibition was 89.7% and 100% respectively.But strains of Sc and Sc-All suppressed on Meloidogyne incognita better by 78.2% and 89.2%.It demonstrated that the symbiotic bacteria was showed toxicity on the plant parasitic nematodes.

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备注/Memo

基金项目：国家“十一五”科技支撑计划（2006BAD21B01-15）。

第一作者简介：宋洁(1985-)，女，硕士，主要从事农田有害生物控制研究。E-mail:songjieyabaobao@163.com。

更新日期/Last Update: 2014-12-29