LI Yi-ran,ZHAO Li-qin,LI Ai-ke,et al.Construction of Detection Method for Water soluble Isoflavone Content in Soybean Milk[J].Soybean Science,2014,33(03):429-433.[doi:10.11861/j.issn.1000-9841.2014.03.0429]
豆浆中水溶性大豆异黄酮检测方法的建立
- Title:
- Construction of Detection Method for Water soluble Isoflavone Content in Soybean Milk
- Keywords:
- HPLC; Isoflavone; Soybeanmilk
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 对4种大豆异黄酮（大豆甙、染料木甙、大豆苷元和染料木素）的高效液相检测程序进行了优化，并结合超高速离心技术，进行了不同豆浆中水溶性大豆异黄酮的含量分析。结果表明：采用C18色谱柱（150 mm×4.6 mm，5.0 μm），流动相A为含0.1%(V/V)乙酸水溶液，流动相B为含0.1%（V/V）乙酸甲醇溶液，进行梯度洗脱，流速为0.8 mL.min-1，检测波长为260 nm，豆浆中的4种异黄酮组分能得到很好的分离，大豆甙、染料木甙、大豆苷元、染料木素的相关系数（R2）分别是0.999 2，0.999 1，0.999 3和0.999 4，线性良好。通过豆浆产品的检测分析发现，不同类型豆浆中水溶性异黄酮含量差异较明显，自制豆浆中的水溶性异黄酮含量显著高于常见市售豆浆。
- Abstract:
- The method for the detection of isoflavone in soybean milks by the High Performance Liquid Chromatography（HPLC）was optimized.And ultra high-speed centrifugation was used for the determination of watersoluble isoflavone contents including Daidzin,Genistin,Daidein and Genistein.The result showed that under the conditions of a C18 column(150 mm × 4.6 mm,5.0 μm),mobile phase A containing 0.1%(V/V)acetic acid in ultrapure aqueous,mobile phase B containing 0.1%(V/V)acetic acid in methanol,a gradient mobile phase at 260 nm,a flow rate of 0.8 mL?min-1,the four different kinds of isoflavones in soybeanmilk could be well separated and detected which good linearities with the correlation coefficient of daidzin,genistin,daidein,genistein were 0.999 2，0.999 1，0.999 3 and 0.9994,respectively.The result also showed that there were significant differences among the four kinds of isoflavone contents in different types of soymilk product.Furthermore,homemade soymilk has a significantly higher isoflavone content than common commercially soymilk.The method constructed can be used for the fast and accurate detection of isoflavone in different kinds of soybean milk.
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备注/Memo
收稿日期：2013-11-26