ZHANG Xiao yan,LU Yan wu,WEI Sheng jun,et al.Effects of Light Quality on Growth,Soy Isoflavone Content and PAL Activity of Soybean Sprouts[J].Soybean Science,2014,33(01):46-52.[doi:10.11861/j.issn.1000-9841.2014.01.0046]
光质对大豆芽苗菜生长和大豆异黄酮含量及PAL活性的影响
- Title:
- Effects of Light Quality on Growth,Soy Isoflavone Content and PAL Activity of Soybean Sprouts
- 文章编号:
- 1000-9841(2014)01-0046-07
- Keywords:
- Soybean sprouts; Light quality; Soy isoflavone; PAL activity
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 采用发光二极管(light emitting diode,LED)精确调制光谱能量分布,以黑暗培养为对照,研究不同光质对大豆芽苗菜品种菜豆6号和苏鲜豆21的生长、大豆异黄酮含量及PAL活性的影响。结果表明:黄光有利于大豆芽苗菜鲜重的增加;随着光质处理时间的延长,两个品种大豆芽苗菜子叶中的大豆异黄酮含量总体呈先增加后降低或持续降低的趋势,而下胚轴中的大豆异黄酮含量则总体呈先降低后增加的趋势;培养至第6天时,下胚轴中的大豆异黄酮含量高于子叶中的含量;与黑暗培养相比,光照处理显著提高了下胚轴中的大豆异黄酮含量,其中UV-B的作用效果最显著,且下胚轴中PAL活性在UV-B下显著提高
- Abstract:
- Light emitting diode(LED)was applied to accurately modulate light spectral energy distribution.The effects of light quality on the growth,total soybean isoflavone content and PAL activity of Caidou 6 and Suxiandou 21 soybean sprouts were studied.Dark was used as the control.The results showed that yellow light promoted the accumulation of the fresh weight.With the increasing of culture time,the total soybean isoflavone content first increased and then decreased or decreased continuously in the cotyledons,while the total soybean isoflavone content first decreased and then increased in the hypocotyls.On the sixth day of culture,the total soybean isoflavone content of both soybean sprouts varieties were higher in hypocotyls than in cotyledons.Compared with the control,light treatment significantly promoted the content of total soybean isoflavone in the hypocotyls,wherein the role of UV-B was the most significant,and UV-B treatment significantly promoted the PAL activity in hypocotyls of both two soybean sprouts varieties.
参考文献/References:
[1]徐茂军,朱睦元,顾青.发芽大豆中异黄酮积累的光诱导作用研究[J].中国粮油学报,2003,18(1):74-77.(Xu M J,Zhu M Y,Gu Q.Light-induced accumulation of isoflavone in soybean sprouts[J].Journal of the Chinese Cereals and Oils Association,2003,18(1):74-77.)
[2]陈宣钦,张乐,徐慧妮,等.大豆异黄酮生物合成关键酶及其代谢工程研究进展[J].中国生物工程杂志,2012,32(7):133-138.(Chen X Q,Zhang L,Xu H N,et al.Key enzymes in soybean isoflavones biosynthesis and its metabolic engineering[J].China Biotechnology,2012,32(7):133-138.)
[3]Ye Y B,Wang Z L,Zhou S Y,et al.Soy germ isoflavones improve menopausal symptoms but have no effect on blood lipids in early postmenopausal Chinese women:a randomized placebo-controlled trial[J].The Journal of the North American Menopause Society,2012,19(7):791-798.
[4]Ma D F,Qin L Q,Wang P Y,et al.Soy isoflavone intake increases bone mineral density in the spine of menopausal women:meta-analysis of randomized controlled trials[J].Clinical Nutrition,2008,27:57-64.
[5]徐春华,张治广,谢明杰.大豆异黄酮的抗氧化和抗肿瘤活性研究[J].大豆科学,2010,29(5):870-873.(Xu C H,Zhang Z G,Xie M J.Research on antioxygenic and antitumor activities of soybean isoflavones[J].Soybean Science,2010,29(5):870-873.)
[6]Boucher B A,Cotterchio M,Anderson L N,et al.Use of isoflavone supplements is associated with reduced postmenopausal breast cancer risk[J].International Journal of Cancer,2013,132:1439-1450.
[7]谢灵玲,赵武玲,沈黎明.光照对大豆叶片苯丙氨酸解氨酶(PAL)基因表达及异黄酮合成的调节[J].植物学通报,2000,17(5):443-449.(Xie L L,Zhao W L,Shen L M.Light regulation of the expression of PAL gene in soybean leaves and isoflavone synthesis[J].Chinese Bulletin of Botany,2000,17(5):443-449.)
[8]Ensminger P A,Schaefer E.Blue and ultraviolet-B light photoreceptors in parsley cells[J].Photochemistry and Photobiology,1992,55:437-447.
[9]赵德修,李茂寅,邢建民,等.光质、光强和光期对水母雪莲愈伤组织生长和黄酮生物合成的影响[J].植物生理学报,1999,25(2):127-132.(Zhao D X,Li M Y,Xing J M,et al.Effects of light on cell growth and flavonoids biosynthesis in callus cultures of Saussurea- medusa-Maxim[J].Acta Phytophysiologica Sinica,1999,25(2):127-132.)
[10]张颖.绿瓣大豆芽菜生产技术研究[D].南京:南京农业大学,2008:1-2.(Zhang Y.Study on the technology of green soybean sprouts production[D].Nanjing:Nanjing Agricultural University,2008:1-2.)
[11]崔瑾,徐志刚,邸秀茹.LED在植物设施栽培中的应用和前景[J].农业工程学报,2008,24(8):249-253.(Cui J,Xu Z G,Di X R.Applications and prospects of light emitting diode in plant protected culture[J].Transactions of the Chinese Society of Agricultural Engineering,2008,24(8):249-253.)
[12]Nhut D T,Don N T,Tanaka M.Light-emitting diodes as an effective lighting source for in vitro?banana culture[C]//Jain S M,H-ggman H.Protocols for micropropagation of woody trees and fruits.Berlin:Springer Netherlands,2007:527-541.
[13]Chi H Y,Roh J S,Kim J T,et al.Light quality on nutritional composition and isoflavones content in soybean sprouts[J].Korean Journal of Crop Science,2005,50:415-418.
[14]Kim E H,Kim S H,Chung J I,et al.Analysis of phenolic compounds and isoflavones in soybean seeds(Glycine max?L.Merrill)and sprouts grown under different conditions[J].European Food Research and Technology,2006,222:201-208.
[15]Phommalth S,Jeong Y S,Kim Y H,et al.Effects of light treatment on isoflavone content of germinated soybean seeds[J].Journal of Agricultural and Food Chemistry,2008,56:10123-10128.
[16]鞠兴荣,袁建,汪海峰.三波长紫外分光光度法测定大豆异黄酮含量的研究[J].食品科学,2001,22(5):46-48.(Ju X R,Yuan J,Wang H F.Study on determination of soybean isoflavones content by three-wavelength,UV spectrophotometry[J].Food Science,2001,22(5):46-48.)
[17]刘金,魏景立,刘美艳,等.早熟苹果花青苷积累与其相关酶活性及乙烯生成之间的关系[J].园艺学报,2012,39(7):1235-1242.(Liu J,Wei J L,Liu M Y,et al.The relationships between the enzyme activity of anthocyanin biosynthesis,ethylene release and anthocyanin accumulation in fruits of precocious apple cultivars[J].Acta Horticulturae Sinica,2012,39(7):1235-1242.)
[18]Lister C E,Lancaster J E.Developmental changes in enzymes of flavonoid biosynthesis in the skins of red and green apple cultivars[J].Journal of the Science of Food and Agriculture,1996,71,313-330.
[19]杜洪涛,刘世琦,蒲高斌.光质对彩色甜椒幼苗生长及叶绿素荧光特性的影响[J].西北农业学报,2005,14(1):41-45.(Du H T,Liu S Q,Pu G B.Effects of light qualities on growth and chlorophyll fluorescence parameters in leaves of color pepper seedling[J].Acta Agriculturae Boreali-occidentalis Sinica,2005,14(1):41-45.)
[20]Islam M O,Matsui S,Ichihashi S.Effects of light quality on seed germination and seedling growth of?Cattleya orchids in vitro[J].Journal of the Japanese Society for Horticultural Science,1999,68(6):1132-1138.
[21]刘晓英,徐志刚,焦学磊,等.可调LED光源系统设计及其对菠菜生长的影响[J].农业工程学报,2012,28(1):208-121.(Liu X Y,Xu Z G,Jiao X L,et al.Design on LED flexible light system and its effect on growth of spinach[J].Transactions of the CSAE,2012,28(1):208-121.)
[22]Kozak D.The influence of light quality and BA on in vitro?growth and development of Gardenia jasminoides?Ellis[J].Acta Scientiarum Polonorum.Hortorum Cultus,2011,10(4):65-73.
[23]Dyachok J,Zhu L,Liao F Q,et al.SCAR mediates light-induced root elongation in Arabidopsis?through photoreceptors and proteasomes[J].The Plant Cell,2011,23(10):3610-3626.
[24]唐大为.LED光源不同光质对黄瓜幼苗生长及生理生化的影响[D].兰州:甘肃农业大学,2010.(Tang D W.Effect of different LED light quality on growth and physiological and biochemical of cucumber seedlings[D].Lanzhou:Gansu Agricultural University,2010.)
[25]刘文科,杨其长,邱志平,等.LED光质对豌豆苗生长、光合色素和营养品质的影响[J].中国农业气象,2012,3(4):500-504.(Liu W K,Yang Q C,Qiu Z P,et al.Effects of LED light quality on growth,photosynthetic pigments and nutritional quality of pea seedlings[J].Chinese Journal of Agrometeorology,2012,3(4):500-504.)
[26]Schmidt B,Quirmbach M.Content of selected isoflavones in plants and plant cell cultures of soybean[J].Current Topics in Phytochemistry,2002,5:91-98.
[27]Ribeiro M L L,Mandarino J M G,Carr-o-panizzi M C,et al.?β-glucosidase activity and isoflavone content in germinated soybean radicles and cotyledons[J].Journal of Food Biochemistry,2006,30:453-465.
[28]孙君明,丁安林,沈黎明.光照对大豆幼苗组织中异黄酮含量和分布的影响[J].植物学报,1998,40(11):1015-1021.(Sun J M,Ding A L,Shen L M.Light effect on the tissue contents and distribution of isoflavones in the developing seedling of soybean[J].Acta Botanica Sinica,1998,40(11):1015-1021.)
[29]Lee S J,Ahn J K,Khanh T D,et al.Comparison of isoflavone concentrations in soybean(Glycine max L.Merrill)sprouts grown under two different light conditions[J].Journal of Agricultural and Food Chemistry,2007,55(23):9415-9421.
[30]陈振,康玉兄.豆类种子及萌发过程中功效性成分研究概述[J].中国食物与营养,2012,18(10):27-32.(Chen Z,Kang Y X.Research advancement of bioactive compounds in legumes seeds and their germinated products[J].Food and Nutrition in China,2012,18(10):27-32.)
[31]刘炳仁,于瑞兰,刘坤,等.豆芽优质生产新技术[M].天津:天津科学技术出版社,2006:7.(Liu B R,Yu R L,Liu K,et al.New technology of high-production soybean sprouts[M].Tianjin:Tianjin Science and Technology Press,2006:7.)
[32]卢洪,习岗,杨初平,等.应用BPAT研究UV-B诱导下大豆愈伤异黄酮含量的变化[J].大豆科学,2010,29(5):863-866.(Lu H,Xi G,Yang C P,et al.Reveal isoflavone contents changes of soybean callus induced by UV-B radiation with Biophoton Analytical Technology[J].Soybean Science,2010,29(5):863-866.)
[33]王莉,史玲玲,刘玉军.不同光质对长鞭红景天悬浮细胞生长及苯丙氨酸解氨酶活性的影响[J].林业科学,2007,43(6):52-56.(Wang L,Shi L L,Liu Y J.Effects of different light treatments on growth and PAL activity of the suspension-cultured cells of Rhodiola fastigiata[J].Scientia Silvae Sinicae,2007,43(6):52-56.)
[34]梁宗锁,李倩,徐文晖.不同光质对丹参生长及有效成分积累和相关酶活性的影响[J].中国中药杂志,2012,37(14):2055-2060.(Liang Z S,Li Q,Xu W H.Effects of different light quality on growth,active ingredients and enzymes activities of Salvia miltiorrhiza[J].China Journal of Chinese Materia Medica,2012,37(14):2055-2060.)
[35]Kubashek W L,Shirley B W,McKillop A,et al.Regulation of flavonoid biosynthetic genes in germinating Arabidopsis?seedlings[J].The Plant Cell,4(10):1229-1236.
[36]Sreelakshmi Y,Sharma R.Differential regulation of phenylalanine ammonia lyase activity and protein level by light in tomato seedlings[J].Plant Physiology and Biochemistry,2008,46(4):444-451.?
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备注/Memo
基金项目:国家自然科学基金(31171998);国家基础科学人才培养基金(J1310015)。
第一作者简介:张晓燕(1988-),女,硕士,主要从事植物光生物学研究。E-mail:201116009@njau.edu.cn。
通讯作者:崔瑾(1974),女,博士,教授,主要从事设施栽培环境调控机理研究。E-mail:cuijin@njau.edu.cn。