[1]何恩铭,沈瑞池,王 伟,等.紫外分光光度法测定豆渣提取物中大豆异黄酮含量的研究[J].大豆科学,2013,32(06):818-820.[doi:10.11861/j.issn.1000-9841.2013.06.0818]
　HE En-ming,SHEN Rui-chi,WANG Wei,et al.Determination of Isoflavones in Extraction of Soybean Dregs Using Ultraviolet Spectrophotometry[J].Soybean Science,2013,32(06):818-820.[doi:10.11861/j.issn.1000-9841.2013.06.0818]
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紫外分光光度法测定豆渣提取物中大豆异黄酮含量的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年06期 页码: 818-820 栏目: 出版日期: 2013-12-25
Title:
Determination of Isoflavones in Extraction of Soybean Dregs Using Ultraviolet Spectrophotometry
作者:
?何恩铭; 沈瑞池; 王 伟; 常 强; 管齐扬
?（福建省亚热带植物研究所，福建省植物生理生化重点公共实验室，福建 厦门 361006）
Author(s):
?HE En-ming; SHEN Rui-chi; WANG Wei; CHANG Qiang; GUAN Qi-yang
?(Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant，Fujian Institute of Subtropical Botany，Xiamen 361006，China)
关键词:
大豆异黄酮; 紫外分光光度法; 豆渣
Keywords:
Soybean isoflavones; Ultraviolet spectrophotometry; Soybean dregs
DOI:
10.11861/j.issn.1000-9841.2013.06.0818
文献标志码:
A
摘要:
?试验建立了一种测定豆渣提取物中大豆异黄酮含量的快速分析方法。以染料木素为标准品，采用紫外分光光度计在260 nm处测定豆渣提取物中大豆异黄酮的含量，回归方程为C=8.052 7A-0.053 2，R2=0.999 5，线性范围：1.31～11.79 μg·mL-1，平均回收率100.13%，相对标准偏差0.86%。该方法具有操作简单、快速，测定准确率高、重现性好等特点，适于生产上快速测定大批量豆渣提取物样品中大豆异黄酮的含量。
Abstract:
?A method was established for fast determination of isoflavones in extraction of soybean dregs.Genistein was selected as the reference standard and the samples of extraction of soybean dregs were determined at 260 nm using ultraviolet spectrophotometry.Regression equation was:C=8.052 7A-0.053 2，R2=0.999 5，the linear range was 1.31-11.79 μg·mL-1，and the average recovery was 100.13%，RSD=0.86%.The method has the virtue of simplicity and rapid of operation，high accuracy and good reproducibility，which can be used in production for the rapid determination of isoflavones in extraction of soybean dregs，and provide a reference in soybean isoflavones determination of other samples.

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备注/Memo

?厦门市重大科技创新平台项目（3502Z20131004）；福建省亚热带植物研究所科研基金（2013S004）;福建省公益类科研院所基本科研专项（2011R1101022-3）；国家自然科学基金（30970126）。

更新日期/Last Update: 2014-04-04