[1]张红梅,董俊彤,白 云,等.野生大豆GsRNF12基因的克隆及表达特性研究[J].大豆科学,2013,32(06):750-754.[doi:10.11861/j.issn.1000-9841.2013.06.0750]
点击复制

野生大豆GsRNF12基因的克隆及表达特性研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年06期 页码: 750-754 栏目: 出版日期: 2013-12-25
Title:
Isolation and Expression Patterns of GsRNF12 Gene in Glycine soja
作者:
?张红梅1; 董俊彤1; 白 云2; 邓 馨3; 肖莉杰1; 费志宏4; 方淑梅1; 韩毅强1; 王北艳1
?（1.黑龙江八一农垦大学 生命科学技术学院，黑龙江 大庆 163319； 2.大庆市质量技术监督局标准化研究所，黑龙江 大庆 163319； 3.中国科学院 植物研究所，北京 100093；4.黑龙江八一农垦大学 农学院，黑龙江 大庆 163319）
Author(s):
?（1.College of Life Science and Technology,Heilongjiang Bayi Agricultural University，Daqing 163319,China；2.Daqing Bureau of Quality and Technical Supervision Standardization,Daqing 163319,China；3.Institute of Botany,Chinese Academy of Sciences，Beijing 100093,China；4.College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing 163319,China）
关键词:
野大豆; GsRNF12基因; 基因表达; 逆境胁迫
Keywords:
Glycine soja; GsRNF12 gene; Gene expression; Stress
DOI:
10.11861/j.issn.1000-9841.2013.06.0750
文献标志码:
A
摘要:
?采用RT-PCR的方法，根据栽培大豆的RNF12基因全长设计特异引物，从野生大豆克隆到GsRNF12基因。序列分析表明，该基因含723 bp的开放阅读框（ORF），编码240个氨基酸，GsRNF12蛋白在178~220氨基酸处有典型的C4HC3结构域，属于C3HC4锌指蛋白家族。利用实时荧光定量PCR对野生大豆GsRNF12基因的表达特性进行分析，结果表明：野生大豆GsRNF12基因对高盐、干旱、低温、ABA、SA胁迫处理均存在不同程度的应答响应；GsRNF12基因在根、茎、叶的表达有差异性，其中在根中表达量最大。
Abstract:
?The GsRNF12 gene from Glycine soja was amplified by RTPCR.Its full length cDNA I was 723 bp and contained a open reading frame（ORF）,encoding a polypeptide of 240 amino acid.GsRNF12 protein in 178220 amino acid has a typical C4HC3 domain structure,belonging to the C3HC4 zinc finger protein family.The expression patterns of GsRNF12 gene were analyzed using quantitative realtime PCR.The results indicated GsRNF12 gene responded differently to salt,cold,drought,ABA and SA；GsRNF12 had no tissuespecific expression characteristics which expressed in roots,stems and leaves,and the expression in root was higher than that in other tissues.

相似文献/References:

[1]王丽燕.NaCl处理对野大豆生理生化特性的影响[J].大豆科学,2008,27(06):1067.[doi:10.11861/j.issn.1000-9841.2008.06.1067]
　WANG Li-yan.Effects of NaCl Stress on Physiological and Biochemical Characters of Glycine soja[J].Soybean Science,2008,27(06):1067.[doi:10.11861/j.issn.1000-9841.2008.06.1067]

备注/Memo

?黑龙江省教育厅项目（11521202）。

更新日期/Last Update: 2014-02-28