[1]尹学哲,金延华,金梅花,等.大豆异黄酮对内毒素及D-氨基半乳糖所致小鼠急性肝损伤的保护作用[J].大豆科学,2013,32(04):544-547.[doi:10.11861/j.issn.1000-9841.2013.04.0544]
　YIN Xue-zhe,JIN Yan-hua,JIN Mei-hua,et al.Protective Effects of Soy Isoflavones on Acute Liver Injury Induced by Lipopolysaccharide and D-galactosamine in Mice[J].Soybean Science,2013,32(04):544-547.[doi:10.11861/j.issn.1000-9841.2013.04.0544]
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大豆异黄酮对内毒素及D-氨基半乳糖所致小鼠急性肝损伤的保护作用

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年04期 页码: 544-547 栏目: 出版日期: 2013-08-25
Title:
Protective Effects of Soy Isoflavones on Acute Liver Injury Induced by Lipopolysaccharide and D-galactosamine in Mice
作者:
?尹学哲1; 金延华1; 金梅花2; 金 明2
?1.延边大学 附属医院，吉林 延吉 133000；2.延边大学 医学院，吉林 延吉 133000
Author(s):
?YIN Xue-zhe1;  JIN Yan-hua1;  JIN Mei-hua2;  JIN Ming2
?1.Affiliated Hospital of Yanbian University,Yanji 133000,China;2.Medical College of Yanbian University,Yanji 133000,China
关键词:
大豆异黄酮; 肝损伤; 抗炎; 抗凋亡
Keywords:
Soybean isoflavones; Liver injury; Anti-inflammatory; Anti-apoptotic
分类号:
%151.41
DOI:
10.11861/j.issn.1000-9841.2013.04.0544
文献标志码:
A
摘要:
?为研究大豆异黄酮对D-氨基半乳糖（GalN）和内毒素（LPS）联合所致小鼠急性肝损伤的保护作用，将试验小鼠按体重随机分为5组，即正常组、模型组、水飞蓟素组（阳性对照组）及大豆异黄酮高、低剂量组。每日给药1次，连续7 d。除正常组外，其余小鼠腹腔注射GalN及LPS建立急性肝损伤模型，苏木素伊红（HE）染色法观察肝组织病理学变化，ELISA法检测血清肿瘤坏死因子-α（TNF-α），比色法检测血清谷丙转氨酶（ALT）、谷草转氨酶（AST）以及肝脏一氧化氮（NO）、过氧化脂质（LOOH）、丙二醛（MDA）含量和Caspase-3、Caspase-8活化水平。结果表明：大豆异黄酮显著降低GalN及LPS联合诱发的急性肝损伤小鼠血清ALT、AST活性，减轻肝组织病理损伤，降低血清TNF-α和肝NO水平，降低肝LOOH和MDA水平，降低肝组织Caspase-3和Caspase-8活化水平。说明大豆异黄酮对GalN和LPS联合诱导的小鼠急性肝损伤具有保护作用，其机制可能与其抗炎、抗氧化活性和抗肝细胞凋亡作用有关。
Abstract:
?For the purpose of studying the protective effect of soy isoflavones on acute liver injury induced by D-galactosamine(GalN)and lipopolysaccharide(LPS)in mice,the tested mice were randomly assigned to 5 groups:normal control,model control,silymarin(positive control),as well as high and low dose groups of soy isoflavones.Animals were treated once daily for 7 days.GalN and LPS were given intraperitoneally to the mice of groups,then the pathological changes was detected with hematoxylin & eosin(HE)staining,tumor necrosis factor-α(TNF-α)was detected by ELISA method,and the alanine aminotransferase(ALT),aspartate aminotransferase(AST),lipid hydroperoxide(LOOH),malondialdehyde(MDA),nitric oxide(NO),and the activation of Caspase-3 and Caspase-8 were detected by the colorimetric method.The results showed that the soy isoflavones reduced the serum ALT and AST,alleviated the hepatic lesions induced by GalN and LPS,decreased serum TNF-α and hepatic NO levels,reduced the hepatic LOOH and MDA contents,and decreased the Caspase-3 and Caspase-8 activations of liver tissues.It is suggested that soy isoflavones have protective effect on acute liver injury induced by GalN and LPS in mice,probably via the anti-inflammatory,antioxidative and anti-apoptotic ability.

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备注/Memo

?基金项目：国家自然科学基金（30360113）。

更新日期/Last Update: 2014-04-04