[1]魏 强,李鲁华,王春艳,等.大豆凝集素le2基因RNA干扰表达载体构建及转化的研究[J].大豆科学,2013,32(03):306-309.[doi:10.11861/j.issn.1000-9841.2013.03.0306]
　WEI Qiang,LI Lu-hua,WANG Chun-yan,et al.Construction of RNAi Expressed Vector of Soybean Agglutinin le2 Gene and Transform Research[J].Soybean Science,2013,32(03):306-309.[doi:10.11861/j.issn.1000-9841.2013.03.0306]
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大豆凝集素le2基因RNA干扰表达载体构建及转化的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年03期 页码: 306-309 栏目: 出版日期: 2013-06-25
Title:
Construction of RNAi Expressed Vector of Soybean Agglutinin le2 Gene and Transform Research
作者:
?魏 强; 李鲁华; 王春艳; 付永平; 马 建; 曲 静; 王丕武
?吉林农业大学 农学院，吉林 长春 130118
Author(s):
?WEI Qiang; LI Lu-hua; WANG Chun-yan; FU Yong-ping; MA Jian; QU Jing; WANG Pi-wu
Agronomy College of Jilin Agricultural University，Changchun 130118,China
关键词:
RNA干扰; 大豆凝集素; 载体构建; 遗传转化
Keywords:
RNAi; Soybean agglutinin; Construction of vector; Transform
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2013.03.0306
文献标志码:
A
摘要:
?以构建RNA干扰表达体系，探索改良大豆品质方法为目的，根据GenBank中已知的大豆凝集素le2基因核酸保守序列（AY342212），设计相应引物，克隆le2基因核心保守序列，测序并与原序列比对同源性为99.77%。以质粒p3301-PFNZ-α′作为基础载体，通过亚克隆构建含有RNA干扰元件和bar基因的表达载体pCAMBIA3301-le2RNAi。通过农杆菌转化法将RNA干扰表达载体导入受体大豆吉农28，获得PCR阳性T1代植株5株，种子23粒。研究结果为大豆品质改良奠定了基础。
Abstract:
?For the purpose of constructing RNAi expressed vector,and discussing the method to improve soybean quality,according to known conserved sequence(AY342212)of soybean agglutinin le2 gene in GenBank,right primer was designed and the conserved sequence was cloned.And the homology was 99.77% contrast to the original sequence.Herein,using P3301-PFNA-α’ as the basic vector,the expression vector pCAMBIA3301-le2RNAi was constructed which contained RNA interference vector and bar gene through subcolone.The RNA interference expression vector was then transformed into cotyledon nodes of soybean Jinong 28 by Agrobacterium mediated method.5 positive transgenic plants in T1 generation were confirmed by PCR detection and 23 seeds were harvested from T1 transgenic plants.The results provided a basis for soybean quality improvement.

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更新日期/Last Update: 2014-03-04