[1]石 海,苗淑杰,刘居东,等.施氮对结瘤和非结瘤近等位基因大豆生长和固氮性状的影响[J].大豆科学,2012,31(06):961-965,971.[doi:10.3969/j.issn.1000-9841.2012.06.022]
　SHI Hai,MIAO Shu-jie,LIU Ju-dong,et al.Effects of Nitrogen Application on Growth and Nitrogen Fixation in Nodulation and Non-nodulation Soybean Isolines[J].Soybean Science,2012,31(06):961-965,971.[doi:10.3969/j.issn.1000-9841.2012.06.022]
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施氮对结瘤和非结瘤近等位基因大豆生长和固氮性状的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年06期 页码: 961-965,971 栏目: 出版日期: 2012-12-30
Title:
Effects of Nitrogen Application on Growth and Nitrogen Fixation in Nodulation and Non-nodulation Soybean Isolines
文章编号:
1000-9841（2012）06-0961-05
作者:
石 海1; 2; 苗淑杰1; 刘居东1; 周克琴1
1.中国科学院 东北地理与农业生态研究所 黑土区农业生态重点实验室，黑龙江 哈尔滨 150081；
2.中国科学院 研究生院，北京 100049
Author(s):
SHI Hai1; 2; MIAO Shu-jie1; LIU Ju-dong1; ZHOU Ke-qin1
1.Key Laboratory of Mollisols Agroecology,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Harbin 150081,Heilongjiang;
2.Graduate University of the Chinese Academy of Sciences,Beijing 100049,China
关键词:
C、N代谢; 结瘤; 固氮; 近等位基因大豆; 阈值
Keywords:
Carbon and nitrogen metabolism; Nodulation; Nitrogen fixation; Isoline soybean; Threshold
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.06.022
文献标志码:
A
摘要:
以结瘤和非结瘤近等位基因大豆品系为材料，利用砂培方式，分析了不同供氮水平对大豆生长、碳氮代谢、结瘤和固氮的影响。结果表明：供氮水平直接影响大豆生物量，结瘤大豆在N2水平（54 mg尿素·kg-1砂）时生物量达到最大(9.16 g·株-1)；非结瘤大豆在N4水平（216 mg尿素·kg-1砂）时生物量达到最大(6.36 g·株-1)。植株体内碳氮含量与生物量的变化趋势相同。此外，结瘤品系N2水平大豆根瘤数最多(205个·株-1)，极显著高于除N4以外的其它处理（P<0.01），该水平的共生固氮量最大，为275.92 mg·株-1，且与N3、N4、N5处理间差异极显著（P<0.01）。因此，结瘤和非结瘤大豆对外源氮的需求存在显著差异，该试验条件下的氮阻遏阈值为N2水平，即54 mg尿素·kg-1砂。
Abstract:
To illustrate the influence of nitrogen fertilizer application on the growth and nitrogen fixation in soybean,the study on the effect of the different N application levels on growth,carbon and nitrogen metabolism,nodulation and nitrogen fixation in soybean under sand culture condition,was carried out with nodulation and non-nodulation soybean isolines.The results showed that N application levels directly impacted soybean biomass.The biomass of nodulation soybean reached the maximum value of 9.16 g·plant-1 at N2 level(54 mg urea·kg-1 sand),while the non-nodulation soybean reached the maximum of 6.36 g·plant-1 at N4 level(216 mg urea·kg-1 sand).There was similar trend of carbon and nitrogen metabolism affected by N level.In addition,nodule number reached the largest number(205 per plant)at N2 level,and there were significant differences between N2 and other treatment,exception of N4 level(P<0.01).The amount of symbiotic nitrogen fixation reached the maximum of 275.92 mg·plant-1 at N2 level,and there were significant differences between N2 and N3(N4 or N5)(P<0.01).These results showed that there was obvious difference between nodulation and non-nodulation soybean isoline to the demand of exogenous nitrogen.To take full advantage of symbiotic nitrogen fixation of soybean,nitrogen repression threshold was N2 level(54 mg·kg-1 sand)under this experiment conditions.

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备注/Memo

基金项目：中国科学院青年人才项目（KZCX2-EW-QN306）；国家自然科学基金项目（41101219）。

第一作者简介：石海（1985-），男，在读硕士，研究方向为大豆结瘤固氮生理。E-mail: shihailone@126.com。
通讯作者：苗淑杰（1975-），女，博士，副研究员，硕士生导师，主要从事大豆结瘤固氮和土壤碳循环等方面的研究。E-mail: miaoshujie@126.com。

更新日期/Last Update: 2014-08-20