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[1]阚贵珍,童振峰,胡振宾,等.野生大豆荚粒相关性状QTL定位[J].大豆科学,2012,31(03):333-340.[doi:10.3969/j.issn.1000-9841.2012.03.001]
　KAN Gui-zhen,TONG Zhen-feng,HU Zhen-bin,et al.Mapping QTLs for Yield Related Traits in Wild Soybean (Glycine soja Sieb.and Zucc.)[J].Soybean Science,2012,31(03):333-340.[doi:10.3969/j.issn.1000-9841.2012.03.001]

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野生大豆荚粒相关性状QTL定位

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷期数: 2012年03期页码: 333-340 栏目: 出版日期: 2012-03-25

Title:: Mapping QTLs for Yield Related Traits in Wild Soybean (Glycine soja Sieb.and Zucc.)

文章编号:: 1000-9841（2012）03-0333-08

作者:: 阚贵珍; 童振峰; 胡振宾; 张丹; 张国正; 喻德跃; 南京农业大学大豆研究所，国家大豆改良中心，作物遗传与种质创新国家重点实验室，江苏南京 210095

Author(s):: KAN Gui-zhen; TONG Zhen-feng; HU Zhen-bin; ZHANG Dan; ZHANG Guo-zheng; YU De-yue; Soybean Research Institute,Nanjing Agricultural University,National Center for Soybean Improvement,National Key Laboratory for Crop Genetics and Germplasm Enhancement,Nanjing 210095,Jiangsu,China

关键词:: 野生大豆; 荚粒相关性状; QTL定位

Keywords:: G.sojaSeed and pod traits; QTL analysis

分类号:: S565.1

DOI:: 10.3969/j.issn.1000-9841.2012.03.001

文献标志码:: A

摘要:: 利用复合区间作图法和混合线性模型复合区间作图法，对野生大豆江浦野生豆-5和栽培大豆南农06-17所得的F_2∶3家系（2008、2009年）及F_2∶4家系（2009年）的单株有效荚数、单株粒重、百粒重3个荚粒相关性状进行QTL分析。结果表明：复合区间作图法检测到27个QTL，混合线性模型复合区间作图法检测到18个加性显性QTL和13对上位性QTL，2种方法共同检测到17个QTL，其中12个QTL（qEPP-H-1、qEPP-Lb-1、qSWP-La-1、qSWP-Lb-1、qSW-B1-1、qSW-B2-1、qSW-D1b-1、qSW-H-1、qSW-H-2、qSW-I-2、qSW-Lb-1和qSW-Ma-1）在2 a或2个世代稳定表达，qEPP-H-1、qEPP-Lb-1和qSWP-Lb-1的增效等位基因来源于野生大豆。研究结果为野生大豆优异等位基因的发掘、栽培大豆遗传基础的拓宽以及大豆产量分子标记辅助育种提供理论依据。

Abstract:: The objective of our study was to map quantitative trait loci(QTL)of yield related traits from G.soja that could improve the soybean.F_2∶3 and F_2∶4populations(316 lines)were derived from a cross between Glycine soja ‘Jiangpuyeshengdou-5’ and Glycine max ‘Nannong 06-17’.Three seed and pod traits including effective pods per plant(EPP),seed weight per plant(SWP)and 100-seed weight(SW)of 2008 F_2∶3,2009 F_2∶3 and 2009 F_2∶4were mapped by composite interval mapping(CIM)and mixed-model-based composite interval mapping(MCIM).Twenty-seven QTLs were detected by CIM.Eighteen additive-dominant QTLs and thirteen pairs of epistatic QTLs were detected by MCIM.Seventeen QTLs were detected by CIM and MCIM.Twelve QTLs(qEPP-H-1、qEPP-Lb-1、qSWP-La-1、qSWP-Lb-1、qSW-B1-1、qSW-B2-1、qSW-D1b-1、qSW-H-1、qSW-H-2、qSW-I-2、qSW-Lb-1andqSW-Ma-1)were stably detected in two years or in two generations.There were three QTLs:qEPP-H-1,qEPP-Lb-1 and qSWP-Lb-1,with positive effects from wild soybean.These results can provide a theoretical basis for excavating excellent genes,broadening cultivated soybean genetic basis and molecular marker assisted breeding of yield.

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备注/Memo

基金项目：国家重点基础研究发展计划项目（2010CB125906）；南京农业大学青年科技创新基金（KJ2011004）。

第一作者简介：阚贵珍（1978-），女，讲师，在读博士，研究方向为大豆分子遗传育种。E-mail：kanguizhen@njau.edu.cn。

通讯作者：喻德跃（1965-），男，教授，博士生导师，研究方

向为植物分子遗传与生物技术。E-mail：dyyu@njau.edu.cn。

更新日期/Last Update: 2014-08-16