ZHAO Tian-hong,CAO Yan-hong,WANG Yan,et al.Effects of Ozone Stress on Root Morphology and Reactive Oxygen Species Metabolism in Soybean[J].Soybean Science,2012,31(01):52-57.[doi:10.3969/j.issn.1000-9841.2012.01.012]
臭氧胁迫对大豆根系形态和活性氧代谢的影响
- Title:
- Effects of Ozone Stress on Root Morphology and Reactive Oxygen Species Metabolism in Soybean
- 文章编号:
- 1000-9841（2012）01-0052-06
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 利用开顶式气室（OTCs）对大豆进行臭氧（O3）胁迫试验，研究了高浓度O3对大豆根系体积、根瘤数、根瘤干重、根干重等根系形态指标以及膜脂过氧化程度、活性氧、抗氧化酶活性等活性氧代谢指标的影响。结果表明：在高浓度O3处理下，根系体积、根及根瘤干重、根瘤数降低，根系MDA含量、相对电导率显著上升，膜脂过氧化程度加剧；超氧阴离子（O·2）产生速率和H2O2含量增加；O3胁迫处理的SOD活性在分枝期高于CK处理，其余各时期均低于CK处理；CAT活性除在开花期低于CK处理外，其它时期均高于CK处理；POD活性呈先升后降的趋势，各个时期均高于CK处理。高浓度O3导致大豆抗氧化系统的氧化损伤，对大豆表现为伤害效应，从而抑制了大豆根系的正常生长发育。
- Abstract:
- In recent years，the elevated ozone has become a serious problem which has some significant effects on plant.The response of morphological characteristics and reactive oxygen species metabolism in roots of soybean to elevated ozone concentrations was investigated in this paper.Soybean grown in open top chambers(OTCs)exposed to ambient air(O3=40 nL·L-1)and elevated ozone(O3=110±10 nL·L-1).Root volume，nodule number，root and nodule dry weight，relative electrical conductivity and malonaldehyde(MDA)，hydrogen peroxide(H2O2)and superoxide anion(O-2)production rate，activities of superoxide dismutase(SOD)，catalase(CAT)and peroxidase(POD)were determined.The results showed that the root volume and root dry weight per plant decreased，then the nodule number and nodule dry weight per plant also declined with ozone concentration rising.Compared to CK,elevated ozone treatment significantly increased MDA and H2O2 content(P<0.05)，relative electrical conductivity and O-2 production rate(P<0.05).SOD activity was higher than CK at branching stage but lower than CK at the other stages under elevated ozone stress.Activity of CAT was lower than CK at flowering stage but higher at the other stages.Activity of POD increased in the early period and decreased in the late period，which was higher than CK at all growing stages.It can be concluded that the ozone stress has negative effects on soybean roots on account of oxidative damage，which restrains the growth and development of soybean roots.
参考文献/References:
[1]Liu L，Sundet J K，Liu Y，et al.A study of tropospheric ozone over China with a 3-D global CTM model[J].Terrestrial，Atmospheric and Oceanic Sciences，2007，18(3):515-545.
[2]Prentice I，Farquhar G，Fasham M，et al.The carbon cycle and atmospheric carbon dioxide[R].Contributions of Working Group I to the Third Assessment Report of the Intergovernmental Panel on Climate Change,2001:183-238.
[3]Feng Z Z，Yao F F，Chen Z，et al.Response of gas exchange and yield components of field grown Triticum?aestivum?L.to elevated ozone in China[J].Photosynthetica，2007，45:441-446.
[4]金明红，黄益宗.臭氧污染胁迫对农作物生长与产量的影响[J].生态环境，2003，12(4):482-486.(Jin M H，Huang Y Z.Review of crops damaged and yield loss by ozone stress[J].Ecology and Environment，2003，12(4):482-486.) [5]Gaucher C，Costanzo N，Widden P，et al.Response to an ozone gradient of growth and enzymes implicated in tolerance to oxidative stress in Acer saccharum(Marsh.)seedlings[J].Annual Forestry Science，2006，63:387-397.
[6]郑飞翔，王效科，张巍巍，等.臭氧胁迫对水稻光合作用与产量的影响[J].农业环境科学学报，2009，28(11):2217-2223.(Zheng F X，Wang X K，Zhang W W，et al.Influences of elevated ozone on rice photosynthesis and yield[J].Journal of Agro-Environment Science，2009，28(11):2217-2223.)
[7]张巍巍，赵天宏，王美玉，等.臭氧浓度升高对银杏光合作用的影响[J].生态学杂志，2007，26(5):45-649.(Zhang W W，Zhao T H，Wang M Y，et al.Effects of elevated ozone concentration on Ginkgo biloba?photosynthesis[J].Chinese Journal of Ecology，2007，26(5):645-649.) [8]Gerosa G，Marzuoli R，Rossini M，et al.A flux-based assessment of the effects of ozone on foliar injury，photosynthesis，and yield of bean(Phaseolus vulgaris?L.cv.Borlotto Nano Lingua di Fuoco)in open-top chambers[J].Environmental Pollution，2009，157:1727-1736.
[9]谢居清，郑启伟，王效科，等.臭氧对原位条件下水稻叶片光合、穗部性状及产量构成的影响[J].西北农业学报，2006，15(3):27-30.(Xie J Q，Zheng Q W，Wang X K，et al.Effect of ozone on photosynthesis of rice leaves，ear character and yield component in Situe[J].Acta Agriculturae Boreali-occidentalis Sinica，2006，15(3):27-30.) [10]阮亚男，何兴元，陈玮，等.臭氧浓度升高对油松抗氧化系统活性的影响[J].应用生态学报，2009，20(5):1032-1037.(Ruan Y N，He X Y，Chen W，et al.Effects of elevated O3concentration on anti-oxidative enzyme activities in Pinus tabulaeformis.[J].Chinese Journal of Applied Ecology，2009，20(5):1032-1037.）
[11]郑有飞，胡程达，吴荣军，等.臭氧胁迫对冬小麦光合作用、膜脂过氧化和抗氧化系统的影响[J].环境科学，2010，31(7):1643-1651.(Zheng Y F，Hu C D，Wu R J，et al.Effects of ozone stress upon winter wheat photosynthesis，lipid peroxidation and antioxidant systems[J].Environmental Science，2010，31(7):1643-1651.) [12]Kasurinen A，Gonzales P K，Riikonen J，et al.Soil CO2efflux of two silver birch clones exposed to elevates CO2 and O3 levels during three growing seasons[J].Global Change Biology，2004，10:1654-1665.
[13]薛应龙.植物生理学实验手册[M].上海:上海科学技术出版社，1985:67-69.(Xue Y L.Phytophysiology experiments manual[M].Shanghai：Shanghai Science and Technology Press，1985：67-69.)
[14]邹琦.植物生理学实验指导[M].北京:中国农业出版社，2000.(Zou Q.Plant physiology experiment guide[M].Beijing:Agricultural Press，2000.)
[15]李玲.植物生理学模块实验指导[M].北京:科学出版社，2009.(Li L.Plant physiology module experiment guide[M].Bei-jing:Science Press，2009.) [16]张巍巍，郑飞翔，王效科，等.臭氧对水稻根系活力、可溶性蛋白含量与抗氧化系统的影响[J].植物生态学报，2009，33(3)：425-432.(Zhang W W，Zheng F X，Wang X K，et al.Effects of ozone on root activity，soluble protein content and antioxidant system in Oryza sativa roots[J].Chinese Journal of Plant Ecology，2009，33(3):425-432.)
[17]徐玲，赵天宏，胡莹莹，等.高浓度臭氧对春小麦膜脂过氧化和抗氧化系统的影响[J].干旱地区农业研究，2008，26(2)：74-78.(Xu L，Zhao T H，Hu Y Y，et al.Effect of ozone on membrane lipid and antioxidation system of wheat leaves[J].Agricultural Research in the Arid Areas，2008，26(2):74-78.)
[18]于涛.臭氧浓度升高对银杏抗氧化能力的影响[J].现代农业科技，2010(10):186-192.(Yu T.Effect of ozone on oxidation resistance of ginkgo[J].Modern agricultural sciences and technology,2010(10):186-192.)
[19]郑启伟，王效科，冯兆忠，等.臭氧对原位条件下冬小麦叶片光合色素、膜脂过氧化的影响[J].西北植物学报，2005，25(11):2240-2244.(Zheng Q W，Wang X K，Feng Z Z，et al.Ozone effects on chlorophyll content and lipid peroxidation in the situ leaves of winter wheat[J].Acta Botanica Boreali-occidentalia Sinica，2005，25(11):2240-2244.)
[20]杨铁钊，殷全玉，丁永乐，等.烟草气孔特性、抗氧化酶活性与臭氧伤害的关系[J].植物生态学报，2004，28(5):672-679.(Yang T Z，Yin Q Y，Ding Y L，et al.Relationships between ozone injury and stoma parameters and activities of antioxidant enzyme[J].Acta Phytoecologica Sinica，2004，28(5):672-679.)
[21]郭水良，方芳，强胜.不同温度对七种外来杂草生理指标的影响及其适应意义[J].广西植物，2003，23(1)：73-76.(Guo S L，Fang F，Qiang S.Influences of temperature on physiological indices of seven exotic weeds and their adaptive significance[J].Guihaia,2003，23(1):73-76.)
相似文献/References:
[1]刘轶鸥,王 岩,刘 波,等.O3浓度升高和UV-B辐射增强对大豆叶片抗氧化酶活性及POD同工酶的影响[J].大豆科学,2013,32(02):202.[doi:10.3969/j.issn.1000-9841.2013.02.014]
LIU Yi-ou,WANG Yan,LIU Bo,et al.Combined Effects of Elevated O3 Concentration and UV-B Radiation on Anti-Oxidative Enzymes Activities and POD Enzymes Isozymes of Soybean[J].Soybean Science,2013,32(01):202.[doi:10.3969/j.issn.1000-9841.2013.02.014]
[2]孙庆元,张雪崧,赵略,等.尿素酶抑制剂NBPT对大豆根系的影响[J].大豆科学,2008,27(01):92.[doi:10.11861/j.issn.1000-9841.2008.01.0092]
SUN Qing-yuan,ZHANG Xue-song,ZHAO Lue,et al.Effect of Urease Inhibitor NBPT on Growth of Soybean Roots[J].Soybean Science,2008,27(01):92.[doi:10.11861/j.issn.1000-9841.2008.01.0092]
[3]王湦曼,王丽红,周青.双酚A对大豆幼苗根系生长及体内含量的影响[J].大豆科学,2015,34(04):624.[doi:10.11861/j.issn.1000-9841.2015.04.0624]
WANG Sheng-man,WANG Li-hong,ZHOU Qing.Effects of BPA on Root Growth and BPA Content in Soybean Roots at Seedling Stage[J].Soybean Science,2015,34(01):624.[doi:10.11861/j.issn.1000-9841.2015.04.0624]
[4]金剑 刘晓冰 王光华.大豆Glycine max (L .)Merrill 根系研究进展[J].大豆科学,2002,21(03):223.[doi:10.11861/j.issn.1000-9841.2002.03.0223]
Jin Jian Liu Xiaobing Wang Guang hua.ADVANCES IN ROOT RESEARCHOF SOYBEAN Glycine max (L.)MERRILL[J].Soybean Science,2002,21(01):223.[doi:10.11861/j.issn.1000-9841.2002.03.0223]
备注/Memo
基金项目：国家自然科学基金（30970448，30500069）；辽宁省高等学校优秀人才支持计划（2011）。