XUE Xiao-yun,FENG Rui-hua,GUAN Da-wei,et al.Screening and Analysis for Efficient Co-inoculation System of Soybean Rhizobia and Plant Growth-promoting Rhizobacteria[J].Soybean Science,2011,30(04):613-620.[doi:10.11861/j.issn.1000-9841.2011.04.0613]
大豆根瘤菌与促生菌复合系筛选及机理研究
- Title:
- Screening and Analysis for Efficient Co-inoculation System of Soybean Rhizobia and Plant Growth-promoting Rhizobacteria
- 文章编号:
- 1000-9841(2011)04-0613-08
- 关键词:
- 大豆根瘤菌; 植物根际促生菌(PGPR); 双接种; 筛选
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 通过溶磷和生长素分泌的定性测定,从大豆根际土壤及实验室保藏菌株中分离筛选植物根际促生细菌(PGPR),采用大豆根瘤菌和促生菌双接种的蛭石盆栽试验,初筛获得能够促进大豆植株生长、提高大豆根瘤菌结瘤固氮作用的促生菌5株。进一步的土壤盆栽复筛实验表明,筛选得到的5株促生菌与大豆根瘤菌进行双接种,能够在阜阳、济宁和延安的3种土壤中明显提高大豆的根瘤数量、根瘤干重、植株干重和全氮含量,并且能够促进植株对磷素的吸收。溶磷能力定量测定表明,5株促生菌均具有溶解无机磷的能力,有的菌株具有较高的溶磷能力;高效液相色谱法测定促生菌发酵液的成分,结果显示5株促生菌具有产生植物激素和有机酸的能力。经过16S rRNA 基因序列分析,所分离的根际促生菌1-3,P7和P13分别属于芽孢杆菌属(Bacillus sp.)、叶杆菌属(Phyllobacterium sp.)和中华根瘤菌属(Sinorhizobium sp.)。
- Abstract:
- Plant growth promoting rhizobacteria(PGPR)were screened from soybean rhizosphere soil and collected strains through qualitative analysis of phosphate solubilization and IAA.Five PGPR strains were acquired,which improved nodulation on soybean when co-inoculated with soybean rhizobia in vermiculite pot experiment.Furthermore in the soil pot experiment with Fuyang,Jining and Yanan’s soils,the co-inoculation of soybean rhizobia with five PGPR strains significantly increased the number of nodules,nodule dry weight,plant dry weight,total N and P contents.The result of quatitive determination of phosphate displayed that five PGPR strains dissolved inorganic phosphorus efficiently.Analysis of fermentation with HPLC showed that 5 isolated strains secreted plant hormones and organic acids.Through 16S rRNA gene sequence analysis,three PGPR 1-3,P7 and P13 strains isolated from soybean rhizosphere soil were belonged to Bacillus sp.,Phyllobacterium sp.and Sinorhizobium sp.,respectively.
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备注/Memo
基金项目:现代农业产业技术体系建设专项资助项目(nycytx-0O4);中央级公益性科研院所科研业务费专项资助项目(2010-34)。