ZENG Wei-ying,LIANG Jiang,CHEN Yuan,et al.Genetic Diversity Analysis of New Wild Soybean Collection in Guangxi[J].Soybean Science,2011,30(03):379-383.[doi:10.11861/j.issn.1000-9841.2011.03.0379]
广西新收集野生大豆资源的遗传多样性分析
- Title:
- Genetic Diversity Analysis of New Wild Soybean Collection in Guangxi
- 文章编号:
- 1000-9841(2011)03-0379-05
- Keywords:
- Guangxi; Wild soybean; Genetic diversity; SSR
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 利用23对SSR引物对广西新收集的200份野生大豆资源进行遗传多样性分析。结果表明:23个SSR位点共扩增出137个多态性带,每个位点等位变异数目范围为3~11个,平均为5.96个;Simpson指数分布范围为0.503~0.834,平均为0.689;Shannon-weaver指数分布范围为0.407~1.444,平均为0.683;遗传相似系数分布范围为0.266~0.969,平均为0.720,广西新收集野生大豆材料表现出丰富的遗传多样性。聚类分析结果显示,200份材料分为三大类,各地区材料之间存在着明显的遗传差异,但有些同一地区内的材料也表现出了明显的遗传分化。
- Abstract:
- Two hundred accessions of wild soybean, collected from Guangxi province were used as materials in this study. Twenty-three pairs SSR primers were used to analyze the genetic diversity of these materials. The result showed that one hundred and thirty-seven bands were amplified in twenty-three pairs SSR primers, with each locus has 5.96 bands. Genetic diversity index showed that distribution of Simpson index was 0.503-0.834, distribution of Shannon-weaver index was 0.407-1.444, and the dicesimilarity was 0.266-0.969, which suggestted that wild soybean collected from Guangxi had abundant genetic diversity. Clustering analysis showed that, two hundred accessions of wild soybean were divided into 3 categories. The genetic distance was very close among the materials in one population and also the materials of different populations because of their similar habitat. However, there were genetics differentiations in a same population.
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备注/Memo
基金项目:广西区青年科学基金资助项目(桂科青099171);广西农业科学院基本业务费[200814(基)];现代农业产业技术体系建设专项资金资助项目。