LIU Xiao-yan,YU Chun-miao,GUO Li-dong,et al.Optimization on Solid State Fermentation Conditions of Producing Polypeptide Feed from High-temperature Soybean Meal[J].Soybean Science,2011,30(02):285-289.[doi:10.11861/j.issn.1000-9841.2011.02.0285]
固态发酵高温豆粕制备多肽饲料的最优发酵工艺条件研究
- Title:
- Optimization on Solid State Fermentation Conditions of Producing Polypeptide Feed from High-temperature Soybean Meal
- 文章编号:
- 1000-9841（2011）02-0285-05
- 分类号:
- TS201
- 文献标志码:
- A
- 摘要:
- 以高温豆粕为原料，研究了枯草芽孢杆菌固态发酵法制备多肽饲料的最优发酵工艺。首先对影响固态发酵多肽得率的培养基组成进行了研究，采用正交试验确定了最佳的固态发酵培养基为：豆麸比2∶1，加水量110%，加蜜量4%。然后对影响固态发酵中多肽得率的发酵工艺参数进行了研究，采用响应面分析法(RSA)确定最佳发酵工艺条件为：接种量15%，发酵温度32℃，发酵时间73 h，在此条件下多肽得率为46.29%。
- Abstract:
- The optimal solid state fermentation conditions of polypeptide production feed with Bacillus subtilis from high-temperature soybean meal were studied in this paper. The effects of medium composition on the yield of polypeptide were studied respectively. The orthogonal experiment was designed to obtain optimal fermentation medium, which were soybean meal∶wheat meal 2∶1, adding 110% water and 4% molasses. The effects of fermentation factors on the yield of polypeptide were studied, respectively. The optimal solid state fermentation conditions obtained from response surface methodology were inoculated with 15% of Bacillus subtilis and fermented at 34℃for 72 h, the production rate of soybean polypeptides could reach 46.29%.
参考文献/References:
[1]胡可心,陈光,孙旸.大豆肽的功能特性的研究[J].酿酒,2004（6）：33-34.(Hu K X，Chen G，Sun Y. Research of the physiology activity of soy peptide[J]. Liquor Making, 2004（6）：33-34.) [2]Pandjaitan N, Hettiarachchy N, Ju Z Y. Enrichment of genistein in soy protein concentrate with beta-glucosdase [J].Journal of Food Science,2000, 65:403-407. [3]Tan T W, Zhang M, Wang B W, et al. Screening of high lipase producing Candid?sp.and production of lipase by fermentation[J]. Process Biochemistry，2003,39:459-465. [4]李清丽,王卫国.双酶复合水解谷朊粉制备小肽的工艺条件研究[J].饲料工业，2007,28(11):17-18.(Li Q L, Wang W G. Study on preparation technology of peptide from gluten by double enzymes[J]. Feed Industry,2007, 28(11):17-18.) [5]关海宁,徐桂花,刁小琴.酶法生产脱脂豆粕小肽工艺条件的研究[J].粮油加工，2006(12):55-56.(Guan H N, Xu G H, Diao X Q. Study on preparation technology of peptide from defatted soybean meal by enzyme[J].Cereals and Oils Processing,2006(12):55-56.) [6]李玉珍,林亲录,肖怀秋,等.大豆多肽特性及其应用研究现状[J].中国食品添加剂，2005(6)：91-94.( Li Y Z, Lin Q L,Xiao H Q, et al. Properties and application research state of soybean peptides[J].China Food Additives, 2005(6)：91-94.) [7]李亮.饲用小肽产品的生产工艺[J].中国牧业通讯, 2007(12):7-11.(Li L. Preparation technology of peptide for feeding[J].China Animal Husbandry Bulletin,2007(12):7-11.) [8]刘超,李学如,柳乐,等.固态发酵生产新型蛋白饲料工艺研究[J].粮食与饲料工业，2006(9):29-31. (Liu C,Li X R, Liu L et al. Studies on technology for producing new protein in feeds by solid state fermentation[J]. Cereals and Feed Industry, 2006(9):29-31.) [9]莫重文,黄岗.固态发酵法生产发酵豆粕的研究[J].中国油脂，2007(7):38-40.(Mo C W, Huang G. Production of fermented soybean meal by solid state fermentation[J].China Oils, 2007(7):38-40.) [10]王金斌,马海乐,段玉清,等.混菌固态发酵豆粕生产优质高蛋白饲料研究[J].中国粮油学报，2009,24（2）:120-124.(Wang J B, Ma H L,Duan Y Q, et al. Producing high quality protein feedstuff from soybean meal by mixed culture solid-state fermentation[J]. Journal of the Chinese Cereals and Oils Association，2009,24（2）:120-124.) [11]戚伟,赵树欣,李艳敏,等.混菌发酵生产富肽蛋白饲料工艺条件的研究[J].饲料工业， 2007,28（17）：6-9.( Qi W, Zhao S X, Li Y M, et al. Investigation on the technological condition of production protein feed rich in peptide by mix bacter ia fermentation [J].Feed Industry,2007,28（17）：6-9.) [12]Box G E P, Behnken D W. Some new three level designs for the study of quantitative variables [J]. Technometrics,1960,2:455-467.
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备注/Memo
基金项目：黑龙江省科技攻关资助项目(GB08B401-03)。