ZHANG Hong-ji,Allen G. Xue,Jinxiu Zhang,et al.Biocontrol of Soybean Root Rot caused by F. oxysporum andF. graminearum[J].Soybean Science,2011,30(01):113-118.[doi:10.11861/j.issn.1000-9841.2011.01.0113]
尖镰孢菌和禾谷镰孢菌引起的大豆根腐病生物防治研究
- Title:
- Biocontrol of Soybean Root Rot caused by F. oxysporum andF. graminearum
- 文章编号:
- 1000-9841（2011）01-0113-06
- Keywords:
- F. oxysporumysporum; F. graminearumaminearum; Soybean; Trichoderma; Biocontrol
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 以大豆与禾谷类作物轮作体系中引起大豆根腐病的尖镰孢菌（F. oxysporum）和禾谷镰孢菌（F. graminearum）为研究对象，测试分离于大豆和小麦根部及根际土壤的生防菌对2种病原菌防治效果。结果表明：拮抗试验中所测试的生防菌对2种镰孢菌抑菌效果差异不显著，生防菌HJ-ZT1、HJ-MM7、HJ-ZT2、CH-Tr14、HJ-MM8和CH-Tr18的抑菌率显著高于CH-Tr51、HJ-MM35、HJ-MM9和ACM941。温室条件下生防菌处理对尖镰孢菌和禾谷镰孢菌2种病原菌的防效及对大豆的促生作用均存在差异，CH-Tr12、ACM941、SB24+ HJ-MM7和化学药剂对尖镰孢菌防效效果较好，并很大程度促进植株生长；生防菌CH-Tr14、HJ-MM7和CH-Tr12对禾谷镰孢菌防治效果较好。田间试验中，生防菌处理ACM941+ HJ-MM7使大豆增产10%， CH-Tr14增产9%，CH-Tr12增产8%，与化学对照增产6%差异不显著。
- Abstract:
- The trails were conducted in Eastern Cereal and Oilseed Research Centre Agriculture and Agri-Food Canada, Ottawa. The target of this research was screening the biocontrol treatments against the soybean root rot caused by F. oxysporumy and F. graminearum in crop rotation systems (soybean/wheat/corn ). The experiments demonstrated that F. oxysporum and F. graminearum isolated from soybean roots cowld cause soybean root rot, and the pathogenicity of F. oxysporum was stronger than that of F. graminearum. Thirteen biocontrol strains isolated respectively from soybean roots, wheat roots and two crop’s rhizosphere soil were used in this study, which were supplied by Eastern Cereal and Oilseed Research Centre Agriculture and Agri-Food Canada and Northeast Institute of Geography and Agricultural ecology，CAS. The control efficiency of the biocontrol strains was not significantly different between F. oxysporumy and F. graminearum in the lab, and the control efficiency of ZT1，MM7，ZT2，Tr14， MM8 and Tr18 were higher significantly than Tr51, MM35, MM9 and ACM. In the greenhouse, the restraint efficiency of the biocontrol treatments on two Fusarium, and their promotion growth on soybean were both different significantly. Meanwhile the effects of thirteen biocontrol treatments were also different significantly. Tr12, ACM, SB24+MM7 and chemical CK could restrain F.oxysporum colonization in certain degree and promote the growth of soybean; however Tr14, MM7 and Tr12 could also restrain F. graminearum. The field trails found that biological control agent ACM + MM7 could increase soybean yield by 10% compared with the CK under the soil inoculated by Fusarium, while CH-Tr14, CH-Tr12 and chemical control could also increase the yield by 9%, 8% and 6%, respectively.
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备注/Memo
基金项目：2007 MOE-AAFC PhD RESEARCH PROGRAM (2007年教育部-加拿大农业部联合培养博士生项目)。