[1]杨卫民,刘宝琦,张世珍.甘露醇、过氧化氢和氯化钠处理对黑豆苗期抗旱性的影响[J].大豆科学,2010,29(02):350-353.[doi:10.11861/j.issn.1000-9841.2010.02.0350]
　YANG Wei-min,LIU Bao-qi,ZHANG Shi-zhen.Effect of Mannitol, Hydrogen Peroxide and Chloride Treatment on Drought Resistance of Black Soybean Seedling[J].Soybean Science,2010,29(02):350-353.[doi:10.11861/j.issn.1000-9841.2010.02.0350]
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甘露醇、过氧化氢和氯化钠处理对黑豆苗期抗旱性的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第29卷 期数: 2010年02期 页码: 350-353 栏目: 出版日期: 2010-04-25
Title:
Effect of Mannitol, Hydrogen Peroxide and Chloride Treatment on Drought Resistance of Black Soybean Seedling
文章编号:
1000-9841（2010）02-0350-04
作者:
杨卫民; 刘宝琦; 张世珍
吕梁高等专科学校，山西 吕梁 033000
Author(s):
YANG Wei-min; LIU Bao-qi; ZHANG Shi-zhen
Lüliang Higher College， Luliang 033000, Shanxi,China
关键词:
黑豆幼苗; 水分胁迫; 脯氨酸; 抗旱性
Keywords:
Black soybean seedlings;  Water stress;  Proline;  Drought resistance
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.02.0350
文献标志码:
A
摘要:
将黑豆幼苗用甘露醇处理后，观察其形态特性与生长状况；用过氧化氢、氯化钠分别浸泡黑豆种子，测定幼苗叶片脯氨酸含量，研究不同处理对黑豆抗旱性的影响。结果表明：甘露醇处理造成黑豆幼苗水分胁迫，致使植株较低，株型紧凑，叶直立，叶面积小，叶片薄，叶色淡绿，根毛较长，根系发达并导致叶片水势降低，幼苗的抗旱能力增强；过氧化氢处理使幼苗叶片脯氨酸含量明显下降，幼苗的抗旱性减弱；而氯化钠处理造成幼苗叶片脯氨酸含量显著提高，抗旱性增强。
Abstract:
The black soybean seedlings were sprayed with mannitol, subsequently, their morphological characteristics, growth status were observed; and the soybean seeds were soaked with different concentration of hydogen peroxide(H2O2) and sodium chloride(NaCl) respectively, then the seedling proline content was determined, to study the effect of different treatments on drought resistance of black soybeans. Results indicated that the black soybean seedlings treated by mannitol resulted in lower plant, compacted plant-type，small and thin leaves,and longer root hair , hence, the drought resistance of the seedlings was enhanced. The proline content showed increase-and-then-decrease trend with the increasing of H2O2 and NaCl concentration. Compared with control, NaCl seed soaking treatment significantly increased proline content, while H2O2 treatment decreased proline content. Hence, NaCl seed soaking is helpful for increase drought resistance in soybean.

参考文献/References:

[1]陈立松,刘星辉.作物抗旱鉴定指标的种类及其综合评价[J].福建农业大学学报,1997,26(1):48-55.（Chen Ｌ Ｓ, Liu Ｘ Ｈ. Kinds of index for crop drought resistance identification and comprehensive evaluation[J]. Journal of Fujian Agricultural University,1997, 26 (1): 48-55.）

[2]倪郁,李唯.作物抗旱机制及其指标的研究进展与现状[J].甘肃农业大学学报,2001,36(1):14-22.（Ni Ｙ,Li Ｖ.The status and recent development in crop drought-resistant mechanism index[J]. Journal of Gansu Agricultural University, 2001, 36 (1): 14-22.）

[3]Melis A. Photosystem Ⅱ Damage and repair cycle in chloroplasts:What modulatethe rate of photodamage in vivo[J] .Trends in Plant Science, 1999:130-135 .

[4]Messina F J,Durham S L. Trade off between plant growth and defense:A comparison of sagebrush populations[J].Oecologia, 2002, 131(1):43-51.

[5]Liu F, Stützel H. Biomass partitioning,specific leaf area ,and water use efficiency of vegetable amaranth (Amaranthusspp.)in response to drought stress[J] .Scientia Horticulturae, 2004, 102 (1):15-27 .

[6]Marcelo S M,Alex-Alan F A,Fabio P G,et al. Leaf gas exchange,chlorophyll fluorescence and growth responses of Genipa Americanaseedlings to soil flooding[J] .Environmental and Experimental Botany, 2003, 50:221-231 .

[7]江龙.作物抗旱性的研究方法[J].贵州农业科学,1999,27(5):70-72.（Jiang Ｌ．Study on the method of the drought-resistant crops [J]. Guizhou Agricultural Sciences, 1999，27 (5):70-72.）

[8]胡标林,李名迪,万勇,等.我国水稻抗旱性鉴定方法与指标研究进展[J].江西农业学报, 2005,17(2):56-60.（ Hu Ｂ L,Li M D, Ｗan Ｙ, et al. Chinese rice drought resistance identification methods and indicators of progress in the [J]. Jiangxi Journal of Agricultural Sciences, 2005, 17 (2): 56-60.）

[9]张志良,翟伟菁.植物生理学实验指导（第三版）[M].北京：高等教育出版社，2007:6-7.（Zhang Z L, Zhai W J. Plant physiology experiments guide (Third edition)[M].Beijing：Higher Education Press，2007:6-7.）

[10] 刘萍,李明军.植物生理学实验技术[M].北京：科学出版社，2007:188-191.（Liu P, Li M J. Experimental technology of plant physiology[M].Beijing：Science Press，2007:188-191.）

[11] 杨卫民.植物细胞水势的研讨[J].吕梁高等专科学校学报,2001(1):50-52.(Yang W M. The discussion of plant cells, water potential [J]. Luliang Higher College, 2001(1): 50-52.）

[12] Turner N C.Drought resistance and adaplation to water delicils in crop plant [M].New York:John Wiley and Sons,1979:343-372.

[13] 王锁民.农作物抗旱性及耐热性的简易诊断法—根冠淀粉水解法[J].国外农学-麦类作物,1990(2):40-41.（Wang S M．Drought resistant crops to compile and heat resistance of simple diagnosis method-the root crown starch hydrolysis [J]. Foreign Agronomy-Journal of Triticeac Grops, 1990(2): 40-41.）

[14] 孙祖东,陈怀珠,杨守臻,等.大豆抗旱性研究进展[J].大豆科学,2001,20(3):221-226.（ＳunＺＤ，Chen H Z, Yang S Z ,et al．Advances in soybean drought resistance [J]. Soybean Science, 2001, 20 (3): 221-226.）

[15] 孙彩霞,沈秀瑛.作物抗旱性鉴定指标及数量分析方法的研究进展[J].中国农学通报,2002,18(1):49-51.（Ｓun C Ｘ, Shen Ｘ Ｙ. Crop drought identify indicators and quantitative analysis methods [J]. China Agronomy Bulletin, 2002, 18 (1): 49-51.）

[16] 路贵和,安海润.作物抗旱性鉴定方法与指标研究进展[J].山西农业科学,1999,27(4):39-43.（Lu Ｇ Ｈ, An H R.Progress on crop drought resistance identification methods and indicators [J]. Shanxi Agricultural Sciences, 1999, 27 (4): 39-43.）

[17]龚明.作物抗旱性鉴定方法与指标及其综合评价[J].云南农业大学学报,1989,4(1):73-81.（Gong M.Crop drought resistance identification methods and indicators and comprehensive evaluation [J]. Journal of Yunnan Agricultural University, 1989, 4 (1): 73-81.）

[18]穆平,李自超,李春平，等.水、旱稻根系性状与抗旱性相关分析及其QTL定位[J].科学通报,2003,48(20)：2162-2169.（Mu P, Li Z C，Li C P,et al．QTL mapping of root tracts of water and upland rice and their correlation with drought resistance[J]. Chinese Science Bulletin, 2003, 48 (20): 2162-2169.）

[19]杨子光,张灿军,冀天会,等.小麦抗旱性鉴定方法及评价指标研究Ⅴ.苗期抗旱指标的比较研究[J].中国农学通报,2008,24(1)：156-159.（Yang Ｚ Ｇ, Ｚhang Ｃ Ｊ,Yi T H ,et al.Study on resistance drought identify method and evaluation index of wheat V.Drought resistant index of wheat seedling[J]. Agronomic Science, 2008,24(1):156-159.）

[20]杨卫民.黑豆中几种生物活性物质的提取及含量对比分析[J].长春工业大学学报，2006,27(2):78-80.(Yang W M.Extraction and comparative analysis of several bioactive substances from black soybean[J]. Changchun University of Technology, 2006,27(2):78-80.)


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备注/Memo

基金项目：山西吕梁高等专科学校基础重点应用资助项目（2008.06）。

第一作者简介：杨卫民（1960-），男，教授,研究方向为植物化学及植物次生物质代谢。E-mail:yangweimin0318@sina.com。

更新日期/Last Update: 2014-09-13