PAN Hong-bin,GU Chun-mei,SUN Ze-wei,et al.Comparative on Antinutritional Effect of Two Cultivars Raw Soybean in Rats[J].Soybean Science,2010,29(02):310-314.[doi:10.11861/j.issn.1000-9841.2010.02.0310]
不同大豆品种对大鼠抗营养影响的比较
- Title:
- Comparative on Antinutritional Effect of Two Cultivars Raw Soybean in Rats
- 文章编号:
- 1000-9841（2010）02-0310-04
- Keywords:
- Raw soybean; Cultivars; Antinutritional; Rat
- 分类号:
- S816.42
- 文献标志码:
- A
- 摘要:
- 为比较2个不同栽培品种生大豆中抗营养因子对大鼠的影响，以50±2日龄Wister大鼠为实验动物，分别饲喂以吉林30、吉农7品种生大豆和膨化大豆粕（对照组）为主要蛋白源的日粮，测定生长性能，第30天解剖大鼠，颈静脉采血检测血液生理生化指标，内脏器官称重，制作肠道组织切片。结果表明：吉林30日粮组大鼠增重、料肉比、胰腺重量、小肠长度、小肠重量、空肠绒毛长度、空肠隐窝深度、白细胞、血浆尿素氮、谷丙转氨酶和总胆红质与对照组大鼠差异显著（P＜0.05），吉林30日粮组大鼠胰腺重量、空肠隐窝深度（JCD）比吉农7日粮组大鼠高29.1%、14.4%（p＜0.05）。吉林30做日粮对大鼠抗营养的影响大于吉农7。
- Abstract:
- The objective of this study was to investigate the antinutritional effect of two different soybean cultivars in rats. Thirty rats aged 50±2 days were randomly divided into three groups, with each group involved 10 replicates. Test groups were fed on the diets containing raw soybean cultivar Jilin 30 and Jinong 7, respectively. Control group diets containing extruded soybean meal(ESM). The whole experiment duration was 30 days, and individual rat weights and feed intakes were recorded weekly. All rats were slaughtered on the 30th day, the rats were collected blood by jugular vein before dissected, the plasma physiological and biochemistry parameters were detected, pancreas, liver, speend, kidney and stomach were collected quickly for weighting， duodenum central region and jejunum1/3 and ileum1/2 were collected to making histological section for light microscope to inspected, respectively.Results showed the gain weight, feed intake，the pancreas weight, small intestine length, small intestine weight,the jejunum villus height(JVH),the jejunum crypt depth(JCD), white blood(cell) count(WBC), plasma urea nitrogen(PUN), glutamate-pyruvate transaminase (GPT) and total bilirubin (TBIL)of the rats fed on the diets containing Jilin 30 raw soybean were significantly different compared to the control group(P<0.05). The pancreas weight and jejunum villus height(JVH) of rats fed on the diets containing Jilin 30 raw soybean were 29.1% and 14.4% (P<0.05) higher than those fed on Jinong 7. Results suggest the antinutritional effect of Jilin 30 in feeding rats is higher than that of Jinong 7.
参考文献/References:
[1]Osborn T B，Mendel L B.The use of soybean as food[J]. Journal of Biological Chemistry, 1917,32(12)：369-387. [2]Liener I E. Implications of antinutritional components in soybean foods[J]. Critical Reviews in Food Science and Nutrition,1994,34(1):31-67. [3]Wang T，Xu R. Effect of Colostrum feeding on intestinal development in newborn pigs[J]. Biology of the Neonate,1996,70(6)：339-348. [4]Grant G, Dorward PM, Buchan W C, et al.Consumption of diets containing raw soya beans (Glycine max), kidney beans (Phaseolus vulgaris), cowpeas (Vigna unguiculata) or lupin seeds (Lupinus angustifolius) by rats for up to 700 days: effect on body composition and organ weights[J]. British Journal of Nutrition ,1995, 73(1):17-29. [5]Li Z, Li D, Qiao S, et al. Anti-nutritional effects of a moderate dose of soybean agglutinin in the rats [J].Archives for Tierernahr，2003,57(4):267-277. [6]Tang S, Li D, Qiao S, et al. Effects of purified soybean agglutinin on growth and immune function in rats[J].Archives of Animal Nutrition， 2006,60(5):418-426. [7]Roy D M,Schneeman B O.Effect of soy protein,casein and trypin inhibitor on cholesterol,bile acids and pancreatic enzymes in mice[J]. Animal Feed Science and Technology,1981,111(5): 878-885. [8]Jordinson M, Deprez P H, Playford R J, et al. Soybean lectin stimulates pancreatic exocrine secretion via CCK-A receptors in rats[J]. American Journal of Physiology - Gastrointestinal and Liver Physiology,1996,270(4):653-659. [9]Li Z, Li D, Qiao S. Effects of soybean agglutinin on nitrogen metabolism and on characteristics of intestinal tissues and pancreas in rats[J].Archives for Tierernahr，2003,57(5):369-380. [10] Donatucli D A. The role of the lectin on the nutritional toxicity of raw legumes[D]. Minneapolis: University of Min-nesota,1983. [11] Pusztai A, George G, Gelencser E, et al. Effects of an orally administered mistletoe (type-2 KIP)lectin on growth, body compostition, small intestinal structure and insulin levels in young rats[J].Journal Nutritional Biochemistry,1998,9(1):31-36. [12] Grant G. Antinutritional effects of soybean: A Review. Prog [J].Food Nutrition Science,1989, 13(2):317-348. [13] Ishiguro M,Nakashima H,Tanabe S,et al. Interaction of toxic lectin ricin with epithelial cells of rat small intestine in vitro[J]. Chemical and Pharmaceutical Bulletin，1992,40(2):441-445. [14] Huisman J,Jansman A J M.Dietary effects and analytical aspects of antinutritional factors in peas(Pisum sativum)common bean(Phaseolus vulgaris) and soybeans (Glycine max L) in monogastric farm animals.A literaturereview[J]. Nutrition Abstracts & Reviews， 1991,61(B)：901-921. [15] Bardocz S, Duguid T J,Brown D S, et al.The importance of dietary polyaes in cell regeneration and growth[J]. Nutrition, 1995, 73(6):819-828. [16] Qin G.Elst E R，Bosch M W,van der Pole A F B . Thermal processing soybean, studies on the inactivation of antinutritional factors ad effects on oral digestibility in piglets [J] Animal Feed Science and Technology,1996, 57(4):313-324. [17] Pull S P,Pueppke S G,Hymowitz T,et al. Soybean lines lacking 120 000 Da seed lectin[J].Science,1978,200:1277-1278. [18] Pusztai A，Bardocz S．Biological effects of plant lectins on the gastrointestinal tract：metabolic consequences and applications [J].Trends Glycosci Glycotechnol,1996,8(41):149-165.
相似文献/References:
[1]王大刚,胡国玉,李杰坤,等.黄淮大豆品种（系）生育期组划分的研究初报[J].大豆科学,2013,32(05):629.[doi:10.11861/j.issn.1000-9841.2013.05.0629]
WANG Da-gang,HU Guo-yu,LI Jie-kun,et al.A Preliminary Report on the Study of Maturity Group Classification of Soybean Varieties(Lines)in HuangHuai[J].Soybean Science,2013,32(02):629.[doi:10.11861/j.issn.1000-9841.2013.05.0629]
[2]赵双进,赵 鑫,唐晓东,等.夏大豆品种高产特性研究[J].大豆科学,2013,32(02):168.[doi:10.3969/j.issn.1000-9841.2013.02.007]
ZHAO Shuang-jin,ZHAO Xin,TANG Xiao-dong,et al.High Yield Characteristics of Summer Sowing Soybean Varieties[J].Soybean Science,2013,32(02):168.[doi:10.3969/j.issn.1000-9841.2013.02.007]
[3]张晓春,陈 红,黄世龙,等.春大豆氮肥施用与大豆品种组合优选研究[J].大豆科学,2012,31(02):255.[doi:10.3969/j.issn.1000-9841.2012.02.019]
ZHANG Xiao-chun,CHEN Hong,HUANG Shi-long,et al.Optimal Combination of Nitrogen Fertilizer and Spring Soybean Varieties in Chongqing[J].Soybean Science,2012,31(02):255.[doi:10.3969/j.issn.1000-9841.2012.02.019]
[4]王大刚,卢为国,马 莹,等.新育成大豆品种对SMV和SCN的抗性评价[J].大豆科学,2009,28(06):949.[doi:10.11861/j.issn.1000-9841.2009.06.0949]
WANG Da-gang,LU Wei-guo,MA Ying,et al.Evaluation of Resistance of Soybean Cultivars to Soybean Mosaic Virus and Soybean Cyst Nematode[J].Soybean Science,2009,28(02):949.[doi:10.11861/j.issn.1000-9841.2009.06.0949]
[5]马淑梅.2006~2010年黑龙江省大豆灰斑病菌生理小种监测及部分主栽品种抗性鉴定[J].大豆科学,2011,30(03):450.[doi:10.11861/j.issn.1000-9841.2011.03.0450]
MA Shu-mei.Monitoring of Physiological Races of Cercospora sojina in Heilongjiang Province from 2006 to 2010 and Resistance Identification of Partial Main Cultivars[J].Soybean Science,2011,30(02):450.[doi:10.11861/j.issn.1000-9841.2011.03.0450]
[6]苗兴芬,徐文平,李灿东,等.东北地区大豆品种脂肪酸组成与含量分析[J].大豆科学,2011,30(03):529.[doi:10.11861/j.issn.1000-9841.2011.03.0529]
MIAO Xing-fen,XU Wen-ping,LI Can-dong,et al.Analysis of the Fatty Acid Composition of Soybean Varieties in Northeast China[J].Soybean Science,2011,30(02):529.[doi:10.11861/j.issn.1000-9841.2011.03.0529]
[7]辛秀君,于凤瑶,张代军,等.黑龙江省近二十年来育成大豆品种品质性状变化分析[J].大豆科学,2010,29(01):56.[doi:10.11861/j.issn.1000-9841.2010.01.0056]
XIN Xiu-jun,YU Feng-yao,ZHANG Dai-jun,et al.Changes on Quality Characters of Soybean Cultivars Released during 1988 to 2007 in Heilongjiang Province[J].Soybean Science,2010,29(02):56.[doi:10.11861/j.issn.1000-9841.2010.01.0056]
[8]庄陆,王术,王伯伦,等.沈阳地区春小麦下茬复种大豆品种产量与品质的比较[J].大豆科学,2010,29(03):424.[doi:10.11861/j.issn.1000-9841.2010.03.0424]
ZHUANG Lu,WANG Shu,WANG Bo-lun,et al.Comparison of Yield and Quality of Soybean Varieties Following Spring Wheat at Shenyang[J].Soybean Science,2010,29(02):424.[doi:10.11861/j.issn.1000-9841.2010.03.0424]
[9]谢甫绨,S.K.St.Martin,张惠君,等.中国辽宁和美国俄亥俄新老大豆品种的比较研究[J].大豆科学,2010,29(04):601.[doi:10.11861/j.issn.1000-9841.2010.04.0601]
XIE Fu-ti,S.K.St.Martin,ZHANG Hui-jun,et al.Evaluation of Old and Modern Soybean Cultivars in Liaoning and Ohio[J].Soybean Science,2010,29(02):601.[doi:10.11861/j.issn.1000-9841.2010.04.0601]
[10]刘红开,康玉凡.芽用豆类作物诱变效应研究进展[J].大豆科学,2010,29(06):1077.[doi:10.11861/j.issn.1000-9841.2010.06.1077]
LIU Hong-kai,KANG Yu-fan.Advances on Mutagenic Effects of Legumes for Bean Sprouts[J].Soybean Science,2010,29(02):1077.[doi:10.11861/j.issn.1000-9841.2010.06.1077]
备注/Memo
基金项目：国家自然科学基金资助项目（30471251）。