YIN Li-na,DUAN Yu-xi,WANG Yuan-yuan,et al.Screening of Rhizobia Against Soybean Cyst Nematode[J].Soybean Science,2010,29(02):276-279.[doi:10.11861/j.issn.1000-9841.2010.02.0276]
拮抗大豆胞囊线虫根瘤菌的研究
- Title:
- Screening of Rhizobia Against Soybean Cyst Nematode
- 文章编号:
- 1000-9841（2010）02-0276-04
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 对传统筛选生防根瘤菌的方法进行了改良，建立一种新的生防根瘤菌筛选体系—半根瘤法。利用此法结合根瘤菌回接结瘤鉴定，从全国7个省市土样诱集到的根瘤中，筛选出23株根瘤菌。分别测定其菌悬液、发酵液对大豆胞囊线虫J2的作用。结果表明：处理48 h后，5株根瘤菌菌悬液及发酵液对大豆胞囊线虫J2具有较高击倒率，分别为Snb21、Snb53、Snb92、Snb166和Snb711；处理72 h后，菌株Snb166和Snb711对大豆胞囊线虫J2具有较高致死活性，其中Snb166发酵液72 h处理J2致死率最高，达到84.27%。
- Abstract:
- Soybean Cyst Nematode (Heterodera glycines) is an important root disease which causes significant economic damage to soybean. Rhizobia are bacterial symbionts of Leguminous plants and of advantage for root colonization. In the present study an attempt was made to use improved method namely half a nodule for screening antagonistic Rhizobia against Heterodera glycines. Soil samples were collected from seven provinces in China and soybean root nodules were trapped from the soil sample. Twenty-three stains were screened from soybean root nodules. Effect of H.glycines J2 treated with different strain suspentions and fermentation filtrates was tested. The results showed that Snb21, Snb53, Snb92, Snb166 and Snb711 strains were against H.glycines J2 after 48 h; Snb166 and Snb711 were effective significantly after 72 h, the corrected mortality rate of nematodes of fermentation filtrate with Snb166 were 84.27% after 72 h.
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备注/Memo
基金项目：大豆现代产业技术体系资助项目。