[1]严君,韩晓增,王守宇,等.不同形态氮对大豆根瘤生长及固氮的影响[J].大豆科学,2009,28(04):674-677.[doi:10.11861/j.issn.1000-9841.2009.04.0674]
　YAN Jun,HAN Xiao-zeng,WANG Shou-yu,et al.Effect of Different Forms Nitrogen on Nodule Growth and Nitrogen Fixation in Soybean (Glycine max L.)[J].Soybean Science,2009,28(04):674-677.[doi:10.11861/j.issn.1000-9841.2009.04.0674]
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不同形态氮对大豆根瘤生长及固氮的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年04期 页码: 674-677 栏目: 出版日期: 2009-08-25
Title:
Effect of Different Forms Nitrogen on Nodule Growth and Nitrogen Fixation in Soybean (Glycine max L.)
文章编号:
1000-9841(2009)04-067-04
作者:
严君1; 2; 韩晓增1; 2; 王守宇1; 王树起1; 李晓慧1
1.中国科学院东北地理与农业生态研究所，黑龙江 哈尔滨 150081；
2.东北农业大学农学院，黑龙江 哈尔滨 150030
Author(s):
YAN Jun1; 2; HAN Xiao-zeng1; 2; WANG Shou-yu1; WANG Shu-qi1; LI Xiao-hui1
1.Northeast Institute of Geography and Agricultural Ecology，Chinese Academy of Sciences,Harbin 150081，Heilongjiang；
2 .College of Agriculture，Northeast Agricultural University，Harbin 150030，Heilongjiang，China
关键词:
根瘤; 固氮酶活性; 不同形态氮; 大豆
Keywords:
Nodule; Nitrogenase activity; Different forms nitrogen; Soybean
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.04.0674
文献标志码:
A
摘要:
采用框栽的培养方法，通过施用不同形态氮肥，探讨不同形态氮对大豆根瘤生长、含氮量、氮积累量以及固氮酶活性的影响。结果表明：不同形态的氮源，对根瘤干重、数量有促进作用，同时增加了根瘤中的含氮量和氮积累量。根瘤干重及数量的变化均为铵态氮(N2)>蛋白氮(N4)>硝态氮(N1)>氨基酸态氮(N3)>酰胺态氮(N5)>生物固氮(N0)，其中N0处理的根瘤干重、数量最少，而N2处理的根瘤干重和数量最多。根瘤含氮量的变化为N1>N4>N5>N2>N3>N0，其中N1处理下的根瘤中的根瘤含氮量最高，N1、N4和N5各处理与N0处理间差异显著(P<5%)。而氮积累的变化为N2>N4>N1>N5>N3>N0，各处理与N0处理相比，差异极显著(P<1%)，与含氮量的变化趋势略有不同。添加不同形态的氮源对根瘤固氮酶活性有抑制作用，其变化趋势为N0>N5>N3>N2>N4>N1，N0处理的固氮酶活性最高。
Abstract:
Nitrogen plays an important role in nitrogen fixation of soybean.In this paper，a pot culture experiment was carried out to study the effects of different forms nitrogen on the variation of dry weight (DW)，number，nitrogen content，nitrogen accumulation and nitrogenase activity in nodule of soybean (Glycine max L.) at flowering stage.The results showed that different forms nitrogen promoted nodule formation and growth，which embodied in dry weight and number significantly increased compared with biological nitrogen fixation.The order of the nodule DW and nodule number were NO+-4-N(N2)>Protein-N(N4)>NO-3-N(N1)>Amino Acid-N(N3)>Amide-N(N5)>Biological Nitrogen Fixation(N0)，the least nodule DW and number appeared in N0 treatment，while the most occurred in N2 treatment.Meanwhile different forms nitrogen increased N content and N accumulation in nodule.The trend of the nitrogen content in nodule was as follows：N1>N4>N5>N2>N3>N0，and nitrogen content was the highest in the N1 treatment，moreover，the difference among N0，N1，N4，N5 was significan at 5% level.However，the order of the N accumulation was N2>N4>N1>N5>N3>N0.The different forms nitrogen remarkably affected nitrogenase activity，the trend was N0>N5>N3>N2>N4>N1，and the nitrogenase activity was the highest in N0 treatment.Therefore，different forms nitrogen had different effects on nitrogen fixation of soybean.

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备注/Memo

基金项目：国家科技支撑计划资助项目(2006BAD21B01，2006BAD05B05)；黑龙江省科技攻关资助项目(GB06B101-1-4)。

作者简介：严君(1982-)，女，硕士，研究方向为植物营养。E-mail:yanjun985625@163.com。
通讯作者：韩晓增，研究员。 E-mail:xzhan@neigaehrb.ac.cn。

更新日期/Last Update: 2014-09-22