[1]李先佳,宋清焕,任丽平.紫外辐射对大豆异黄酮含量变化的影响[J].大豆科学,2009,28(02):357-359.[doi:10.11861/j.issn.1000-9841.2009.02.0357]
　LI Xian-jia,SONG Qing-huan,REN Li-ping.Enhanced Ultraviolet-B Radiation Reduced Isoflavones Contents in Soybean[J].Soybean Science,2009,28(02):357-359.[doi:10.11861/j.issn.1000-9841.2009.02.0357]
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紫外辐射对大豆异黄酮含量变化的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年02期 页码: 357-359 栏目: 出版日期: 2009-04-25
Title:
Enhanced Ultraviolet-B Radiation Reduced Isoflavones Contents in Soybean
文章编号:
1000-9841(2009)02-0357-03
作者:
李先佳; 宋清焕; 任丽平
漯河医学高等专科学校，河南 漯河 462002
Author(s):
LI Xian-jia; SONG Qing-huan; REN Li-ping
Luohe Medical College,Luohe 462002， Henan，China
关键词:
UV-B辐射; 大豆异黄酮; 高效液相色谱法
Keywords:
UV-B radiation; Soybean isoflavones; HPLC
分类号:
R284
DOI:
10.11861/j.issn.1000-9841.2009.02.0357
文献标志码:
A
摘要:
研究了UV-B强度增加对大豆异黄酮含量变化的影响，建立高效液相色谱检测方法用于大豆异黄酮的检测。大豆植株经不同辐照度UV-B辐射后，收获成熟籽粒，超声波振荡法提取大豆异黄酮，采用BDS Hypersil C18柱，以含甲醇∶0．4％ 磷酸（30∶70，v／v)洗脱，DAD254 nm检测，内标法定量。结果显示大豆在180 mwm-2UV-B处理后出现了不同程度的伤害，大豆异黄酮含量明显降低，其中Daidzin、Glycitin、Genistin和Daidzein含量分别比对照降低 4.5%、8.6％、1.1%和11.4％，且具有较好的相关性。
Abstract:
The effect of enhanced ultraviolet radiation(UV-B) on soybean isoflavone content was investigated, a high performance liquid chromatography(HPLC) method for the determination of soybean isoflavones was presented. Soybean plants were treated with enhanced UV-B till mature．The harvested soybean seeds were extracted by ultrasonic assisting and then separated on BDS Hypersil C18 column and methanol-0.4％ phosphoric acid solution (30∶70,by volum)．The elute was detected by a UV detector at 254 nm. Results showed the 180 mwm-2?UV-B treatment significantly affects soybean isoflavones，Daidzin，Glycitin，Genistin and Daidzeint decreased by 4.5%，8.6％，1.1% and 11.4％,respectively．The increase of UV-B treatment and the decrease of isoflavones content had good linearity.

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备注/Memo

作者简介：李先佳，男（1977-），讲师，硕士，研究方向为生物活性成分的分离与鉴定。E-mail:nzr110@163.com。

更新日期/Last Update: 2014-10-04