[1]李玉梅,王根林,于洪久,等.呋喃丹降解菌的分离与特性研究[J].大豆科学,2009,28(02):281-284.[doi:10.11861/j.issn.1000-9841.2009.02.0281]
　LI Yu-mei,WANG Gen-lin,YU Hong-jiu,et al.Separating and Characteristics of a Strain Capable of Degrading Carbofuran[J].Soybean Science,2009,28(02):281-284.[doi:10.11861/j.issn.1000-9841.2009.02.0281]
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呋喃丹降解菌的分离与特性研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年02期 页码: 281-284 栏目: 出版日期: 2009-04-25
Title:
Separating and Characteristics of a Strain Capable of Degrading Carbofuran
文章编号:
1000-9841(2009)02-0281-04
作者:
李玉梅1; 2; 王根林3; 于洪久2; 汤晖4; 陶波1; 马凤鸣1
1东北农业大学农学院，黑龙江 哈尔滨 150010；
2黑龙江省农业科学院农村能源研究所，黑龙江 哈尔滨 150086；
3黑龙江省农业科学院土肥所，黑龙江 哈尔滨 150086；
4河北大学生命科技学院，河北 保定 071002
Author(s):
LI Yu-mei1; 2; WANG Gen-lin2; YU Hong-jiu2; TANG Hui3; TAO Bo1; MA Feng-ming1
1Agricultural College of North-East Agricultural University，Harbin 150010，Heilongjiang；
2Country Resource Institute of Heilongjiang Academy of Agricultural Sciences，Harbin 150086，Heilongjiang；
3Soil Fertilizer Institute of Heilongjiang Academy of Agricultural Sciences，Harbin 150086，Heilongjiang；
4College of Life Science，Hebei University，Baoding 071002，Hebei，China
关键词:
呋喃丹; 生物降解; 沙雷氏菌属
Keywords:
Carbofuran; Biodegradation; Serratia sp.
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.02.0281
文献标志码:
A
摘要:
采用富集驯化培养方法，从长期施用呋喃丹的大豆田土壤中分离到1株可高效降解呋喃丹的菌株。生物降解表明，5 d内该菌株生物降解农药率达到94.7%；降解后期培养液采用气质联用分析方法未能检测到原药呋喃丹，检测到有新的化合物生成，而对照培养液中未发现有该类化合物的生成。同时无机盐培养液后期有红色现象生成，可能与呋喃酚开环有关；无细胞提取液加入农药重新培养表明，菌株可通过分泌胞外诱导酶在周质空间内降解农药；经16S rDNA鉴定，初步认为该菌株属于沙雷氏菌属（Serratia sp.）。
Abstract:
One strain that is capable of degrading carbofuran was isolated from the carbofuran acclimated soybean soil by domestication and enrichment.Biodegrading test showed the degraded ration reached 94.7% in five days.By GC-MS analyzing，carbofuran was degraded into carbophenyl mostly，and in inorganic medium，red compound of carol was showed after a given periods，which may be related with the opening loop of carbophenyl.In no cell extracting solution with pesticide，carbofuran was also degraded into carbophenyl，which showed the strain could degrade pesticide by inducing enzyme out of the cell.The strain is a Serratia sp.by 16S rDNA identification.

参考文献/References:

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备注/Memo

基金项目：黑龙江省农科院青年基金资助项目（20060402）。

作者简介：李玉梅（1971-），女，副研究员，博士研究生，主要从事土壤微生物、肥料等方面的研究。

更新日期/Last Update: 2014-10-04