[1]白晶,张必弦,李新玲,等.C2H2型锌指蛋白基因的克隆与序列分析[J].大豆科学,2009,28(01):21-25.[doi:10.11861/j.issn.1000-9841.2009.01.0021]
　BAI Jing,ZHANG Bi-xian,LI Xin-ling,et al.Cloning and Sequence Analysis of a C2H2 type Zinc Finger Protein Gene from Glycine soja[J].Soybean Science,2009,28(01):21-25.[doi:10.11861/j.issn.1000-9841.2009.01.0021]
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C2H2型锌指蛋白基因的克隆与序列分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年01期 页码: 21-25 栏目: 出版日期: 2009-02-25
Title:
Cloning and Sequence Analysis of a C2H2 type Zinc Finger Protein Gene from Glycine soja
文章编号:
1000-9841(2009)01-0021-05
作者:
白晶1; 张必弦2; 李新玲1; 徐香玲1
1哈尔滨师范大学生命科学与技术学院，黑龙江 哈尔滨 150025；
2黑龙江省农业科学院博士后工作站，黑龙江 哈尔滨 150086
Author(s):
BAI Jing1; ZHANG Bi-xian2; LI Xin-ling1; XU Xiang-ling1
1 Life Science and Technology Institute，Harbin Normal University，Harbin 150025，Heilongjiang；
2 Post doctorial Station of Heilongjiang Academy of Agricultural Science，Harbin 150080，Heilongjiang，China
关键词:
野生大豆; C2H2型锌指蛋白; 基因克隆
Keywords:
Wild soybean; C2H2 type zinc finger protein; Gene cloning
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.01.0021
文献标志码:
A
摘要:
根据大豆SCOF-1的CDs区设计特异引物，通过PCR和RT–PCR从野生大豆01-197中克隆到含两个锌指的C2H2型锌指蛋白基因，命名为GjC2H2，GenBank登录号为FJ172776。对其进行核苷酸和氨基酸同源性分析及系统发生分析，结果表明：该基因内部无内含子，长度为702bp，分子量为25.13KD，与其他锌指蛋白基因有很高的同源性，推测其与植物抗逆性有关。
Abstract:
Specific primers were designed according to the soybean SCOF-1 of CDs from the GenBank，C2H2-type Zinc finger protein gene containing two zinc finger，named GjC2H2 was cloned through the PCR and RT-PCR from the wild soybean 01-197，and the GenBank accession number was FJ172776.Homogenesis and phylogeny analysis of nucleotide，amino acid sequence showed that there was no intron in the gene，the length of the gene was 702 bps，molecular weight was 25.13 KD，and the gene and other zinc finger protein gene had a high homology.It is presumed that the gene GjC2H2 is related with stress tolerance in plants.

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备注/Memo

基金项目：黑龙江省重大攻关课题资助项目（GA06B103-10）；哈尔滨师范大学博士科研启动基金资助项目。

作者简介：白晶（1985-），女，硕士研究生，研究方向为基因工程。E-mail:baijing0451@yahoo.com.cn。

更新日期/Last Update: 2014-09-29