[1]段玉玺,于海峰,陈立杰,等.防治大豆胞囊线虫芽孢杆菌初步筛选[J].大豆科学,2008,27(05):811-813,818.[doi:10.11861/j.issn.1000-9841.2008.05.0811]
　DUAN Yu-xi,YU Hai-feng,CHEN Li-jie,et al.Screening of Bacillus sp.Against Soybean Cyst Nematode(Heterodera glycines)[J].Soybean Science,2008,27(05):811-813,818.[doi:10.11861/j.issn.1000-9841.2008.05.0811]
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防治大豆胞囊线虫芽孢杆菌初步筛选

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年05期 页码: 811-813,818 栏目: 出版日期: 2008-10-25
Title:
Screening of Bacillus sp.Against Soybean Cyst Nematode(Heterodera glycines)
文章编号:
1000-9841(2009)05-0811-03
作者:
段玉玺; 于海峰; 陈立杰; 王胜君
沈阳农业大学植物保护学院,北方线虫研究所，辽宁 沈阳 110161
Author(s):
DUAN Yu-xi; YU Hai-feng ; CHEN Li-jie; WANG Sheng-jun
Nematology Institute of Northerm China，Shenyang Agricultural University，Shenyang 110161，Liaoning，China
关键词:
大豆胞囊线虫; 芽孢杆菌; 发酵液; 校正死亡率
Keywords:
Heterodera glycines; Bacillus sp.; Fermentation broth; Correct mortality rate
分类号:
S432+5
DOI:
10.11861/j.issn.1000-9841.2008.05.0811
文献标志码:
A
摘要:
大豆胞囊线虫（Heterodera glycines Ichinohe）是大豆根部的重要寄生线虫，严重威胁大豆生产，而且防治困难。目前人们试图利用生物防治的方法来控制大豆胞囊线虫病害，以达到农业可持续发展的目的。芽孢杆菌是一类重要的微生物资源，该类微生物具有良好的抗逆性，在线虫病害的生物防治方面具有一定的发展前途。采用稀释分离法，从东北和华北等地区采集土样分离获得295株芽孢杆菌，通过筛选获得6株对大豆胞囊线虫有明显抑制作用的菌株，分别是B12、B28、B49、B65、B76和B108。其中B12菌株的菌悬液对大豆胞囊线虫有较强的杀死作用，校正死亡率达到74.03%；B65发酵液，处理48 h后校正死亡率达到85.90%；B12、B65、B49对大豆胞囊线虫抑制率分别达89.50%、89.50 %、88.00 %，均与对照差异显著。这些菌株对大豆胞囊线虫的形成表现出明显的抑制作用，有可能成为非常有应用前景的生防菌株。
Abstract:
The Soybean Cyst Nematode(Heterodera glycines)，causes economically significant damage to soybean in China.Some bacteria Bacillus，are both resistant to advese envirnment and against pathogens.295 Bacillus sp.were isolated from soil samples by diluted separation in Northeast and North China.B12，B28，B49，B65，B76 and B108 strains could suppress Heterodera glycines and strain suspentions of B12 was effective significantly.The corrected mortality rate of nematodes of strain suspentions with B12 were 74.03% after 48 h.The corrected mortality rate of nematodes of fermentation filtrate with B65 were 85.90% after 48 h.The suppressive percentages of cysts hatching of B12，B65 and B49 were 89.50，89.50 and 88.00.The bacterial strains which had effect on the nematode would be used as the bio-control agents on the soybean root rot pathogen and soybean cyst nematode.

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备注/Memo

基金项目：国家自然科学基金资助项目（30673199）；科技部成果转化基金资助项目（05EFN212100059）。

作者简介：段玉玺(1964-),男,博士,教授,主要从事线虫学研究。E-mail:duanyx6407@163.com。

更新日期/Last Update: 2014-10-06