[1]马志民,刘渊,蒋春志,等.大豆脂肪氧化酶同功酶抗原的快速纯化方法[J].大豆科学,2008,27(02):306-309.[doi:10.11861/j.issn.1000-9841.2008.02.0306]
　MA Zhi-min,LIU Yuan,JIANG Chun-zhi,et al.Rapid Approach of Soybean Lipoxygenase’antigen Purification[J].Soybean Science,2008,27(02):306-309.[doi:10.11861/j.issn.1000-9841.2008.02.0306]
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大豆脂肪氧化酶同功酶抗原的快速纯化方法

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年02期 页码: 306-309 栏目: 出版日期: 2008-04-30
Title:
Rapid Approach of Soybean Lipoxygenase’antigen Purification
文章编号:
1000-9841(2008)02-0306-04
作者:
马志民1; 2; 3; 刘渊1; 3; 蒋春志1; 2; 杨春燕1; 2; 马峙英1; 3; 张孟臣1; 2
1 国家大豆改良中心石家庄分中心，河北 石家庄 050031；
2河北省农林科学院粮油作物研究所，河北 石家庄 050031；
3河北农业大学，河北 保定 071001
Author(s):
MA Zhi-min1; 2; 3; LIU Yuan1; 3; JIANG Chun-zhi1; 2; YANG Chun-yan1; 2; MA Zhi-ying1; 3; ZHANG Meng-chen1; 2
1 National Soybean Improvement Center Shijiazhuang Sub-Center，Shijiazhuang 050031,Hebei;
2 Institute of Cereal and Oil Crops，Hebei Academy of Agricultural And Forestry Sciences，Shijiazhuang 050031，Hebei;
3 Agriculture University of Hebei，Baoding 071001，Hebei，China
关键词:
脂肪氧化酶; 非变性PAGE; 电洗脱; 纯化
Keywords:
Lipoxygenase;  Native PAGE;  Electro elution;  Purification
DOI:
10.11861/j.issn.1000-9841.2008.02.0306
文献标志码:
A
摘要:
目前常用的蛋白纯化方法均存在操作复杂、纯化周期长、成本高等缺点，本试验试图探索出一种经济快捷的蛋白纯化方案，且纯化后的蛋白可以用于制备单克隆抗体；以普通大豆品种冀豆12以及脂肪氧化酶缺失近等基因系Suzuyutaka中的Su-1,3，Su-2,3为材料，将大豆脂氧酶非变性PAGE分析与电洗脱技术相结合，纯化脂肪氧化酶同功酶抗原蛋白；回收到具有活性的电泳纯级脂肪氧化酶溶液，回收率达到了与离子交换柱层析相当的水平（49.64%）；本试验探索的方法实现了应用经济快捷的纯化方案获得免疫所需抗原，为尽早获得脂肪氧化酶单克隆抗体并将其应用于实践打下了基础，并为其它蛋白质的快速分离纯化提供了参考。
Abstract:
The common method of purifying protein has the shortcoming of complex operation，time- consuming of purifying，high costing and so on.The objective of this paper is to explore a fast and economical process of protein purification，and the purified protein can be applied to prepare anti-this protein monoclonal antibody.Combining both Native PAGE of the soybean Lipoxygenase and Electro Elution，we purified the lipoxygense from the soybean Jidou No.12 and the Su-1,3，Su-2,2.Lipoxygenase solution is retrieved with electrophoresis degree and recovery rate 49.64% as same of the ion exchange column chromatography.This built a fast and economical approach for antigen purifying，and it is helpful for preparing anti-lipoxygenase monoclonal antibodies and applied to practice even.It also offers important reference to the purification of other protein.

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备注/Memo

基金项目：河北省自然科学基金资助项目（C2005000605）。

作者简介：马志民（1980-），男，研究实习员，硕士，现主要从事甘薯遗传育种研究。

更新日期/Last Update: 2014-10-10