[1]汪立平 　 陈有容 　 齐凤兰.变质豆浆中腐败微生物的分离及其灭杀条件研究*[J].大豆科学,2006,25(03):254-258.[doi:10.11861/j.issn.1000-9841.2006.03.0254]
　Wang Liping Chen Yourong Qi Fenlan.RESEARCH ON SEPARATION AND STERILIZATION CONDITIONOF PUTRID MICROORGANISMS IN TRANSMNTATIVE SOY MILK[J].Soybean Science,2006,25(03):254-258.[doi:10.11861/j.issn.1000-9841.2006.03.0254]
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变质豆浆中腐败微生物的分离及其灭杀条件研究*

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第25卷 期数: 2006年03期 页码: 254-258 栏目: 出版日期: 2006-08-25
Title:
RESEARCH ON SEPARATION AND STERILIZATION CONDITIONOF PUTRID MICROORGANISMS IN TRANSMNTATIVE SOY MILK
作者:
汪立平 　 陈有容 　 齐凤兰
(上海水产大学食品学院, 上海 200090)
Author(s):
Wang Liping 　 Chen Yourong 　 Qi Fenlan
(College of Food Science and Technology , Shanghai Fisheries University , Shanghai 200090)
关键词:
豆浆; 腐败微生物; 正交实验; 灭菌方法
Keywords:
Soy milk ; Putrid microorganism ; Orthogonal experiment ; Sterilization condition
DOI:
10.11861/j.issn.1000-9841.2006.03.0254
文献标志码:
A
摘要:
对 5 种变质豆浆中的腐败微生物进行了分离,并对所分离的腐败微生物进行了感官实验,发现变质豆浆中的主要腐败微生物是三株细菌 ,而酵母 、霉菌未检出 。以二次灭菌的豆浆为培养基,通过正交实验确定了三株细菌中的最难杀灭细菌的有效灭菌方法 :121℃下灭菌 30min, 同时添加300mg /kgNisin,该灭菌条件可使豆浆在 37℃下贮存 30 天时细菌菌落未检出,但继续贮存至 45 天时,细菌菌落数超过 100 cfu/mL 。
Abstract:
In this paper , putrid microorganisms were separated from five transmutative commercial soymilk samples. The result indicated that putrid microorganism were mainly consisted of three bacteria , noyeast and mould were detected. With re-sterilized soy milk as medium , by means of L 9 (32 )orthogonal ex-periment , sterilization condition for the strongest capacity strain of the three separated bacteria was deter-mined:121 ℃ for 30min , 300mg /kg Nisin. With the determinated sterilization method, when soy milkstored for 30 days under 37℃, no bacterium was detected, but the amount of bacterium exceeded 100 cfu/mL on the 45th day.

参考文献/References:

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备注/Memo

基金项目:上海市教育基金会及上海科技成果转化促进会资助项目;上海市重点学科建设资助项目(T1102)　 作者简介:汪立平(1968 -), 女, 博士, 主要从事豆制品、饮料酒的科学与技术研究。E - mail:lpwang @shfu. edu. cn　 致谢:作者衷心感谢丹尼斯克公司赠送乳酸链球菌素 Nisin 样品。

更新日期/Last Update: 2016-12-15