[1]鲍艳霞 　钱之玉 　陈 　钧 　倪孟祥 　王 　萍.豆渣固体发酵产纳豆激酶的工艺优化及其部分酶学性质研究*[J].大豆科学,2005,24(01):43-47.[doi:10.11861/j.issn.1000-9841.2005.01.0043]
　Bao Yanxia Qian Zhiyu Chen Jun Ni Mengxiang Wang Ping.STUDYON THE TECHNOLOGIES AND SOME ENXYME PROPERTY OFNATTO- KINASE PRODUCED FRCM THE SOLID FERMENTATION OF SOYBEAN RESIDUE[J].Soybean Science,2005,24(01):43-47.[doi:10.11861/j.issn.1000-9841.2005.01.0043]
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豆渣固体发酵产纳豆激酶的工艺优化及其部分酶学性质研究*

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第24卷 期数: 2005年01期 页码: 43-47 栏目: 出版日期: 2005-02-25
Title:
STUDYON THE TECHNOLOGIES AND SOME ENXYME PROPERTY OFNATTO- KINASE PRODUCED FRCM THE SOLID FERMENTATION OF SOYBEAN RESIDUE
作者:
鲍艳霞 1 　钱之玉 2 　陈 　钧 3 　倪孟祥 2 　王 　萍 3
(1. 中国药科大学高职学院, 镇江 212003 ;2. 中国药科大学药学院,南京 210038;3. 江苏大学生物与环境工程学院 ,镇江 212013)
Author(s):
Bao Yanxia 1 　Qian Zhiyu 2 　Chen Jun 3 　Ni Mengxiang 2 　Wang Ping 3
(1. Autitude Vocational School of China Pharmaceutical University ,J iangsu Zhenjiang 212003;2. School of Pharmacy of China Pharmaceutical University ,J iangsu Nanjing 210038 ;3.Biological and Environment Engineering School of Jiangsu University , Zhenj iang ,212013)
关键词:
纳豆菌 ; 固体发酵; 纳豆激酶 ; 酶学性质
Keywords:
Natto strain ; Solid fermentation ; Natto - kinase ; Propertieson of soybean residue was conducted. With single - factor and orthogonal test. The results
DOI:
10.11861/j.issn.1000-9841.2005.01.0043
文献标志码:
A
摘要:
　研究纳豆菌固体发酵产纳豆激酶的工艺及其部分酶学性质 。采用单因素和正交试验 ,对以豆渣为原料纳豆菌固体发酵生产纳豆激酶的工艺条件进行优化 ,并利用最佳发酵工艺制备纳豆激酶粗品,对纳豆激酶的部分性质进行研究 。结果表明固体发酵培养基最佳配比 :豆渣 :麸皮 =5 :2,初始含水量 65%,接种量为 10%,初始 pH 为 8. 0, 培养温度 30℃。采用最适培养基和优化工艺,在 250 ml 三角瓶中进行验证实验 ,纳豆激酶的酶的产率可达到 1577U . g- 1。酶学性质研究表明,最适反应温度为 60 ℃,37℃以下稳定, 最适反应 pH 为 8. 0,在 pH7 - 9 溶液中基本稳定 。体外溶栓作用表明, 纳豆激酶溶解纤维蛋白的方式是直接溶解, 而不是通过激活纤溶酶原 。
Abstract:
Study on the technologies and some enzyme property of Natto - kinase produced from the solidfermentatishowed that the optimal medium of solid fermentation was as follows soybean was residue :wheat bran was5∶2 ;initial moisture content was 65%;the inoculum size was 10%;initial pH 8. 0, and temperature was30℃. Natto - kinase activity was 1577 U g- 1when the strain was grown on the optimal medium underthe above culture conditions in 250 mL Erlenmeyer fiask. The optimum temperature and pH for hydrolysisof casein were 60 ℃and 8. 0 respectively. The enzyme was stable up to 37℃,within the pH range of 7 - 9.The fibrinolysis activity was performed by degrading the fibrous protein directly according to the experi-ment in vitro.

参考文献/References:

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备注/Memo

作者简介:鲍艳霞(1968 -), 女, 讲师, 主要从事生物化学教学工作。电话:0511 - 4409317　E- mail:Bao12004@tom. com

更新日期/Last Update: 2016-12-23