[1]燕平梅,畅晓晖 薛文通张 慧 王玉国.小黑豆组织培养的研究*[J].大豆科学,2005,24(01):12-16.
Yan Pingmei Chang Xiaohui Xue Wentong Zang Hui Wang Yuguo.STUDY ON TISSUE CULTUREOF XIAOHEIDOU ZDD2226[J].Soybean Science,2005,24(01):12-16.
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Yan Pingmei Chang Xiaohui Xue Wentong Zang Hui Wang Yuguo.STUDY ON TISSUE CULTUREOF XIAOHEIDOU ZDD2226[J].Soybean Science,2005,24(01):12-16.
小黑豆组织培养的研究*
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
卷:
第24卷
期数:
2005年01期
页码:
12-16
栏目:
出版日期:
2005-02-25
- Title:
- STUDY ON TISSUE CULTUREOF XIAOHEIDOU ZDD2226
- Keywords:
- Yingxian Xiaoheidou ; Tissue culture ; Callus; Cculture media
- 文献标志码:
- A
- 摘要:
- 以应县小黑豆的下胚轴 、子叶、小真叶为试验材料 ,研究不同培养基对小黑豆愈伤组织诱导和器官分化的影响。结果表明 :NAA 和 BA 单独使用不利用小黑豆的离体培养 , 细跑分裂素与生长素结合使用有利于小黑豆愈伤组织的诱导和器官分化 。下胚轴在 Ms +BA2mg /L +IAA0. 5mg /L 、Ms+BA1. 5mg /L +IAA0. 5mg /L Ms +KT2mg /L +NAA0. 2mg /L 培养基中植株再生频率为 46%、46%、16%,在前两种培养基中下胚轴外植体分化出丛生芽。小黑豆下胚轴愈伤组织诱导率、再生植株分化率较子叶、小真叶高。
- Abstract:
- T he effect of variant culture medium on callus inducement and shoot differentiated was studiedwith first lamina , cotyledons, hypocotyls explants of Yingxian Xiaoheidou. The results indicated that BAand NAA had no advantages on tissue culture of Yingxian Xiaoheidou, but combinations of cytokinin andauxin had advantages on callusinducement and organ differentiation.Culture mediums of Ms+BA2mg /L +IAA0. 5mg /LMs+BA1. 5mg /L +IAA0. 5mg /L and Ms +KT2mg /L +NAA0. 2mg /L ,had different rate ofplant regeneration with 46 %,46%, 16%respectively , In the first and second culture medium had got multi-ple shoot 。The rates of callus inducement and plant regeneration of soybean hypocotyls were higher thanthat of lamina and cotyledons.
参考文献/References:
1 敬强, 程大新. 应县小黑豆对大豆孢囊线虫 4 号生理小种抗性的遗传分析[ J] .中国农学通报, 2001, 17(6):12 - 15.2 陆很恒. 黑豆蛋白质的营养价值及利用对策[ J]. 中国商办工业,2003, 2:40-42.3 程林海, 孙毅, 刘少翔. 基因型和激素浓度对大豆植株再生的影响[ J] . 植物学通报, 2001, 18(3):367 - 370.
备注/Memo
作者简介:燕平梅(1968 -), 女, 讲师, 在职博士,研究方向食品生物技术。电话:010- 62397696 E- mail:yanpingmei @sohu. com
更新日期/Last Update:
2016-12-22