Luan XiaoyanChen YiDu w eiguangZhang GuiruMan WeiqunGu XiuzhiLi Hangw eiGuo ShunfeiJiao Pinglan.STUDIES ON THE CHANGES OF PEROXIDAS, POLYPHENDOXIASE AND SUPEROXIDEDISMUTASE IN PLANTS OF DIFFERENT SOYBEAN CULTIVARS LNFECTED BY SMV[J].Soybean Science,2001,20(03):200-203.[doi:10.11861/j.issn.1000-9841.2001.03.0200]
不同抗性大豆品种感染SMV 后过氧化物酶、多酚氧化酶、超氧化物歧化酶的变化分析
- Title:
- STUDIES ON THE CHANGES OF PEROXIDAS, POLYPHENDOXIASE AND SUPEROXIDEDISMUTASE IN PLANTS OF DIFFERENT SOYBEAN CULTIVARS LNFECTED BY SMV
- 文章编号:
- 1000 -9841(2001)03 -0200 -04
- Keywords:
- Soybean Mosaic Virus(SMV); Peroxidase ; Polyphendo xiase ; Superoxid dismutase ; Plant resistance
- 分类号:
- S565.035.3
- 文献标志码:
- A
- 摘要:
- 本文采用两个高抗SMV3 号株系种质哈91R3 -184 、哈91R3 -301 , 两个感病品种合丰25 、黑农16 于真叶期分别接种SMV3 号株系, 在R1 、R3 、R5 期分别测定过氧化物酶(POD)、多酚氧化酶(PPO)、超氧化物歧化酶(SOD)同工酶活性, 结果表明:1 .抗感病品种未接种健株POD 、PPO 酶谱构型没有差异, 只是活性高低有别, 说明抗性与POD 、PPO 活性有关。2 .接种SMV 后感病品种POD 、SOD 酶活性明显高于未接种健株和抗病品种, 其POD 、PPO 部分酶带加宽色深, 部分酶带缺失。3 .各生育阶段抗感品种PPO 、POD 、SOD 酶活性动态的变化, 可作为抗感病品种的生化鉴定指标。
- Abstract:
- Two resistant and tw o susceptible soybean varieties were inoculated w ith Soy bean Mosaic Virus(SMV).The leaves were taken f rom tested varieties at R1 , R3 , R5stages for biochemical analysis .The reasultshowed as follows :1 There w ere no apperent dif ferences in Perox idase(POD)and Polyphenoloxidase(PPO)isozyme zymogram inthe leaves of uninoculated resistant and susceptible soybean , but there w ere differences in POD and PPO activity .2 After inoculation w ith SMV3 POD , PPO and Superoxide Dismutase (SOD)activities w ere increased mo rerapidly in susceptible varieties than those in uninoculated cultivars .There w ere some bands of POD , PPO in susceptible varieties loss in the inoculated compared w ith the uninoculated .3 .There w ere significant dif fereces in the changing of POD , PPO , SOD activities in leaves of resistant and susceptible soybean along g row th proceding .
参考文献/References:
1 庄炳昌, 徐豹, 廖林.接种不同花叶病毒后大豆叶片SOD、POD和蛋白组分的变化[ J] .植物病理学报, 1993 , 23(3):260 -265 .
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备注/Memo
基金项目:黑龙江省自然科学基金资助项目。