[1]李冬梅,周淑莉,李永光,等.转GmGT2B基因大豆的耐盐性分析[J].大豆科学,2017,36(02):213-219.[doi:10.11861/j.issn.1000-9841.2017.02.0213]
　LI Dongmei,ZHOU Shuli,LI Yongguang,et al.Salt Tolerance Analysis of GmGT2B Transgene Soybean[J].Soybean Science,2017,36(02):213-219.[doi:10.11861/j.issn.1000-9841.2017.02.0213]
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转GmGT2B基因大豆的耐盐性分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年02期 页码: 213-219 栏目: 出版日期: 2017-03-20
Title:
Salt Tolerance Analysis of GmGT2B Transgene Soybean
作者:
李冬梅; 周淑莉; 李永光; 张彬彬; 李文滨
（东北农业大学 大豆生物学教育部重点实验室/农业部东北大豆生物学与遗传育种重点实验室，黑龙江 哈尔滨 150030）
Author(s):
LI Dongmei;  ZHOU Shuli;  LI Yongguang;  ZHANG Binbin;  LI Wenbin
（Key Laboratory of Soybean Biology in Chinese Ministry of Education/Agricultural Ministry’s Northeast Areal Key Laboratory of Soybean Biology and Genetic Breeding, Northeast Agricultural University, Harbin 150030, China）
关键词:
转GmGT2B基因; 抗旱大豆; SOD; MDA; 脯氨酸
Keywords:
GmGT2B;  Drought soybean;  SOD;  MDA;  Proline
DOI:
10.11861/j.issn.1000-9841.2017.02.0213
文献标志码:
A
摘要:
采用盆栽试验方法，苗期在盐胁迫条件下，比较GmGT2B转基因大豆与非转基因大豆的损伤程度、超氧化物歧化酶（SOD）活性，丙二醛（MDA）、脯氨酸和K+、Na+含量以及基因表达量变化差异。结果表明：盐胁迫对转基因大豆叶片的伤害程度明显低于对照；SOD活性、脯氨酸含量、丙二醛（MDA）含量在盐胁迫5d后均与对照有显著或极显著差异；植株的Na+含量随盐胁迫时间的增加而升高，在胁迫第8天转基因大豆的Na+含量达到对照约2倍；转基因大豆的基因的表达量呈上调趋势，在第8天达到表达高峰。基因表达量的变化趋势与生理指标SOD活性、脯氨酸含量和Na+含量表现的一致。结果表明GmGT2B基因与盐应答反应有关，其中转GmGT2B大豆株系N11和N24盐害程度较低，该基因和转GmGT2B基因大豆具有较大的应用价值。
Abstract:
Using potted experimental method, we treated the GmGT2Btransgene soybean and the control with 200 mmol?L-1salt stress in seedling stage, and then measured their damage degree, superoxide dismutase(SOD) activity, malondialdehyde(MDA), proline, K+, Na+ content and the changes of gene expression. As a result, after treated with salt stress for 5 days, injury of leaves in transgenic soybean were lighter than the control under salt stress, and the SOD activity, proline content and malondialdehyde(MDA) levels were also significantly or extremely different; Na+content of soybeans increased as salt stress time increased, the N11, N24 of the Na+content reached twice of the control after 8 days under salt stress; GmGT2B gene expression of N11, N22 and N24 were increased and arrived the peak in the 8 day. Gene expression and variation tendency were consistented with physiological indicators, which were SOD activity and proline and K+, Na+content. The result indicated that GmGT2B gene was related to the responses of salt, the extent of injury of GmGT2B transgene soybean were lower than the control with N11 and N24 salt sress, GmGT2B gene and the transgene soybean both had a large breeding value.

参考文献/References:

［1］张素红, 刘立新, 刘忠卓. 水稻耐盐研究与育种进展［J］. 北方水稻, 2009, 39(3): 118-121. (Zhang S M, Liu L X, Liu Z Z. Research and breeding in rice salt［J］. Northern Rice, 2009, 39(3): 118-121.)

［2］张冬梅, 魏迎春, 王菊花. 常规育种与生物技术相结合培育优良农作物新品种［J］. 西藏农业科技, 2003, 24(2): 36-38. (Zhang D M, Wei Y C, Wang J H. Conventional breeding and biotechnology to cultivate fine combination of new varieties of crops［J］. Journal of Agricultural Science and Technology, 2003, 24(2): 36-38.)
［3］Li T X, Zhang Y, Zhang H X, et al. Stable expression of Arabidopsis vacuolar Na+/H+ antiporter gene AtNHX1, and salt tolerance in transgenic soybean for over six generations［J］. Chinese Science Bulletin, 2010, 55(12): 1127-1134.
［4］Trieu A T, Burleigh S H. Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium［J］. The Plant Journal, 2000, 22(6): 531-541.
［5］Liu H, Wang Q, Yu M, et al. Transgenic salt tolerant sugar beet constitutively expressing an Arabidopsis thaliana vacuolar Na+/H+ antiporter gene, AtNHX3, accumulates moresoluble sugar butless salt in storage roots［J］. Plant Cell Environment, 2008, 31(9): 1325-1334.
［6］Li M, Li Y, Li H Q, et al. Over expression of AtNHX5 improves tolerance to both salt and drought stress in Broussonetia papyrifera (L.) Vent［J］. Tree Physiology, 2011, 31(3): 349-357.
［7］Shu ji Y, Francisco J, Beatriz C, et al. Differential expression and function of Arabidopsis thaliana NHX Na+/H+ antiporters in the salt stress response［J］. The Plant Journal, 2002, 30(5): 529-539.
［8］Xie Z M, Zou H F, Lei G, et al. Soybean trihelix transcription factors GmGT2A and GmGT2B improve plant tolerance to abiotic stresses in transgenic Arabidopsis［J］. PLoS One,2009, 4(9): e6898.
［9］Ji W, Zhu Y M, Li Y, et al. Over expression of a glutathione Stransferase gene, GsGST, from wild soybean (Glycine soja) enhances drought and salt tolerance in transgenic tobacco［J］. Biotechnol Lett, 2010, 32: 1173-1179.
［9］李雁杰, 朱丹华, 冯小锋, 等. 含抗草甘膦CP4 EPSPS基因和耐逆Trihelix类转录因子 GmGT2A基因的植物表达载体构建及转化验证［J］. 浙江农业学报, 2015, 27(2): 133-140. (Li Y J, Zhu D H, Feng X F, et al. Construction and functional verification of a plant expression vector containing both herbicide resistant gene CP4 EPSPS and stress tolerance related transcription factor GmGT 2A［J］. Acta Agriculturae Zhejiangensis, 2015, 27(2): 133-140.)
［10］王启明. 干旱胁迫对大豆苗期叶片保护酶活性和膜脂过氧化作用的影响［J］. 农业环境科学学报, 2006, 25(4): 918-921. (Wang Q M. Drought stress on soybean seedling leaf protective enzyme activities and membrane lipid peroxidation influence［J］. Agro Environment Science, 2006, 25(4): 918-921.)
［11］Health R L, Packer L. Photoperoxidation in isolated chloroplasts:I.Kinetics and stoichiometry of fatty acid peroxidation［J］. Archives of Biochemistry and Biophysics, 1968, 125: 1892-1981.?
［12］Gibon Y, Larher F. Cycling assay for nicotinamide adenine din cleotides: NaCl precipitation and ethanol solubilization of the reduced tetrazolium［J］. Analytical Biochemistry, 1997, 251: 153-157.
［13］Troll W, Lindsley L. A photometric method for the determination of proline［J］. The Journal of Biological Chemistry, 1955, 215(2): 655-660.
［14］Wang C, Zhang D W, Wang Y C. A glycine rich RNA binding protein can mediate physiological responses in transgenic plants under salt stress［J］. Molecular Biology Reports,2012, 39 (2): 1047-1053.
［15］Gadjev I, Vanderauwera S, Gechev T S, et al. Transcriptomic footprints disclose specificity of reactive oxygen species signaling Arabidopsis［J］. Plant Physiology, 2006, 141(2): 436-445.
［16］Kasuga M, Liu Q, Miura S, et al. Improving plant drought, salt, and freezing tolerance bygene transfer of a single stress inducible transcription factor［J］. Nature biotechnology, 1999, 17(3): 287-291.

备注/Memo

基金项目： 抗逆转基因大豆新品种培育专项（2014ZX08004-002）；黑龙江省教育厅一般项目（10531012）。

第一作者简介：李冬梅（1980-），女，硕士，实验师，主要从事大豆遗传转化、转基因检测等研究。E-mail：yy841026@163.com。

更新日期/Last Update: 2017-05-14