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[1]尹燕斌,潘校成,蒋洪蔚,等.野生大豆导入系对蛋白质含量相关位点的上位性分析[J].大豆科学,2016,35(03):353-359.[doi:10.11861/j.issn.1000-9841.2016.03.0353]
　YIN Yan-bin,PAN Xiao-cheng,JIANG Hong-wei,et al.Epistatic Analysis for Protein Content Using Wild Soybean Backcross IntrogressiveLines[J].Soybean Science,2016,35(03):353-359.[doi:10.11861/j.issn.1000-9841.2016.03.0353]

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野生大豆导入系对蛋白质含量相关位点的上位性分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷期数: 2016年03期页码: 353-359 栏目: 出版日期: 2016-05-20

Title:: Epistatic Analysis for Protein Content Using Wild Soybean Backcross IntrogressiveLines

作者:: 尹燕斌¹; 潘校成²; 蒋洪蔚³; 魏思明¹; 刘春燕³; 胡国华³; 陈庆山¹; 1. 东北农业大学农学院，黑龙江哈尔滨150030;?
2. 65301 部队副业基地，黑龙江五大连池164100;?
3. 黑龙江省农垦科研育种中心，黑龙江哈尔滨150090

Author(s):: YIN Yan-bin¹; PAN Xiao-cheng²; JIANG Hong-wei³; WEI Si-ming¹; LIU Chun-yan³; HU Guo-hua³; CHENQing-shan¹; 1. Agronomy College，Northeast Agricultural University，Harbin 150030，China;?
2. Sideline Base for 65301 Force of Heilongjiang，Wudalianchi164100，China;?
3. Land Ｒeclamation Ｒesearch and Breeding Center of Heilongjiang，Harbin 150090，China

关键词:: 野生大豆; 回交导入系; 蛋白质含量; 上位性分析

Keywords:: Wild soybean; Backcross introgressive lines; Protein content; Epistasis analysis

分类号:: S565. 1

DOI:: 10.11861/j.issn.1000-9841.2016.03.0353

文献标志码:: A

摘要:: 利用野生大豆ZYD00006 和栽培大豆绥农14 所构建的回交导入系群体BC3F3代为研究材料，选择亲本间存在差异的121 个SSＲ标记对114 个株行材料进行基因型分析; 利用T 测验对含野生大豆纯合双导入位点( 即B-B 位点组合) 植株表型值与绥农14 表型值进行检测，以P≤0. 05 作为阈值，共获得104 对B-B 位点对表型影响显著，再经T测验， 28 对互作位点存在上位性效应，其中正向上位性效应位点10 对，负向上位性效应位点18 对，本研究结果揭示了上位性效应对大豆蛋白质含量性状的重要影响，同时这些位点信息将为大豆高蛋白分子辅助育种研究提供重要的理论基础。

Abstract:: The wild soybean backcross introgressive lines BC3F3was constructed with cultural soybean Suinong 14 as recurrentparent and wild soybean ZYD00006 as donor parent in this study. One hundred and twenty one polymorphism SSＲ markers betweenthe parents were used to analyze the genotypes of 114 lines. The line with double introgression homozygote loci from wildsoybean plants ( B-B loci) and Suinong 14 were analyzed with T-test. With P≤0. 05 as threshold value，108 B-B locus weresignificant on phenotype. And epistasis effect was found at 28 B-B locus. Among them， 10 were positive and 18 were negative.The result of this study have revealed the importance of epistasis effect on soybean protein content，meanwhile the informationof these locus will be the theoretical basis for high protein of soybean breeding.

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备注/Memo

基金项目: 黑龙江省新世纪优秀人才项目( 1252-NCET-004) 。

第一作者简介: 尹燕斌( 1989-) ，男，硕士，主要从事作物遗传育种研究。E-mail: 15546031783@163. com。

通讯作者: 陈庆山( 1973-) ，男，教授，博导，主要从事大豆生物技术研究。E-mail: qshchen@126. com。

更新日期/Last Update: 2016-06-23