[1]蔡燕,周红波,鞠剑锋,等.外源钡离子对脂肪氧合酶好氧催化体系的影响[J].大豆科学,2015,34(06):1053-1056.[doi:10.11861/j.issn.1000-9841.2015.06.1053]
　CAI Yan,ZHOU Hong-bo,JU Jian-feng,et al.The Effects of Exogenous Ba2+?on the Lipoxygenase Catalytic System[J].Soybean Science,2015,34(06):1053-1056.[doi:10.11861/j.issn.1000-9841.2015.06.1053]
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外源钡离子对脂肪氧合酶好氧催化体系的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年06期 页码: 1053-1056 栏目: 出版日期: 2015-12-25
Title:
The Effects of Exogenous Ba2+?on the Lipoxygenase Catalytic System
作者:
蔡燕; 周红波; 鞠剑锋; 吴锦明
南通大学 化学化工学院，江苏 南通 226019
Author(s):
CAI Yan;  ZHOU Hong-bo;  JU Jian-feng;  WU Jin-ming
School of Chemistry and Chemical Engineering，Nantong University，Nantong 226019，China
关键词:
脂肪氧合酶; 钡; 构象; 氢过氧化物
Keywords:
Lipoxygenase;  Ba2+;  Conformation;  Hydroperoxide
DOI:
10.11861/j.issn.1000-9841.2015.06.1053
文献标志码:
A
摘要:
对外源金属钡离子对大豆脂肪氧合酶的紫外光谱和荧光光谱的影响进行了测定，并考察了钡离子对脂氧酶好氧催化大豆油体系反应产率的影响。结果表明：在Ba2+离子加入量为0.75 mmol.L-1时，脂氧酶特征吸收A360从初始的0.1升高至0.4。但Ba2+离子对脂氧酶发射光谱影响甚微，在360 nmol.L-1峰值处与原始谱图比仅下降了约1.2%。在Ba2+离子加入浓度为4 mmol.L-1时，脂氧酶好氧催化豆油体系的产率从8%提高至18%，影响显著。
Abstract:
Lipoxygenase (LOX )can catalyze the oxidation of polyunsaturated fatty acids and their-esters containing the cis,cis-1,4-pentadiene moiety to the corresponding hydroperoxides. The effect of exogenous Ba2+on the conformation and catalytic performance of LOX on soybean oil was observed. The addition of exogenous Cu2+in LOX solvent strengthened the absorbance A360in UV spectrum from 0.1 to 0.4. Exogenous Ba2+could quench the fluorescence excitation spectra of LOX slightly (by 1.2%). When the addition concentration of Ba2+was 4 mmol.L-1, the total yield of soybean oil hydroperoxide catalyzed by LOX increased from 8% to 18%.

参考文献/References:

［1］Cai K, Fang Y, Xia Y M, et al. Effect of exogenous iron on aerobic catalytic mechanism of soybean lipoxygenase［J］. Journal of Molecular Catalysis B: Enzymatic, 2004,32:21-26.

［2］Cai Y, Xu H, Xia YM, et al. The proof of the displacement of endogenous Fe(II) of lipoxygenase by exogenous Cu2+［J］. Applied Biochemistry and Biotechnology,2010, 162(4): 1147-1152.
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备注/Memo

基金项目：江苏省青年自然科学基金（BK20150401）；江苏省高校自然科学研究面上项目(15KJB530010)。

第一作者简介：蔡燕(1983-)，女，博士，讲师，主要研究方向为酶催化研究。E-mail:Yancai2010@ntu.edu.cn。
通讯作者：吴锦明（1958-），男，博士，教授，主要从事有机合成研究。E-mail:wjm@ntu.edu.cn。

更新日期/Last Update: 2016-01-07