[1]石云,孔祥珍,华欲飞.大豆糖蜜上清液中胰蛋白酶抑制剂的去除[J].大豆科学,2015,34(02):298-301.[doi:10.11861/j.issn.1000-9841.2015.02.0298]
　SHI Yun,KONG Xiang-zhen,HUA Yu-fei.Removal of Trypsin Inhibitor from Soy Molasses Supernatant[J].Soybean Science,2015,34(02):298-301.[doi:10.11861/j.issn.1000-9841.2015.02.0298]
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大豆糖蜜上清液中胰蛋白酶抑制剂的去除

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年02期 页码: 298-301 栏目: 出版日期: 2015-04-25
Title:
Removal of Trypsin Inhibitor from Soy Molasses Supernatant
作者:
石云; 孔祥珍; 华欲飞
江南大学 食品学院/食品科学与技术国家重点实验室，江苏 无锡 214122
Author(s):
SHI Yun;  KONG Xiang-zhen;  HUA Yu-fei
School of Food Science and Technology, Jiangnan University/State Key Laboratory of Food Science and Technology,Wuxi 214122, China
关键词:
大豆糖蜜; 胰蛋白酶抑制剂; 肽凝胶电泳; 超滤; 大豆低聚糖
Keywords:
Soy molasses;  Tripsin inhibitor;  Tricine-SDS-PAGE;  Ultrafiltration;  Soy oligosaccharides
分类号:
TS201.2
DOI:
10.11861/j.issn.1000-9841.2015.02.0298
文献标志码:
A
摘要:
胰蛋白酶抑制剂是广泛存在于大豆及大豆制品中的抗营养剂，主要有Kuntiz型和Bowman-Birk型两种，为了得到高品质的产品，需要在加工过程中将其灭活或去除。通过研究大豆糖蜜上清液中胰蛋白酶抑制剂的活性、鉴定抑制剂的种类，采用超滤法将其去除。经测定，固形物含量约10%的大豆糖蜜上清液中胰蛋白酶抑制剂活性约为5.05×105?TIA?g-1蛋白，约为鲜豆奶的5倍。用截留分子量3 kDa的超滤膜将大豆糖蜜上清液中的大分子蛋白富集，通过还原Tricine-SDS-PAGE分析，截留部分在图谱上显示为分子量约10 kDa的模糊的条带。通过用2%巯基乙醇还原再用NEM封闭游离巯基的方式处理样品，使得截留部分在电泳图上显示为分子量约7~8 kDa的两条清晰的条带。综合判断，大豆糖蜜上清液中主要含有Bowman-Birk型胰蛋白酶抑制剂。通过对比超滤效果，选择用截留分子量10 kDa的超滤膜截留胰蛋白酶抑制剂，能够得到无抑制活性的透过液。
Abstract:
Trypsin inhibitor is one kind of antinutrition factor widely spread in soybean and soybean products, which mainly consist of Kuntiz type and Bowman-Birk type. In order to get high-quality products, soybean trypsin inhibitor is better to be inactivated or removed.Through analyzing the activity and identifying the type of trypsin inhibitor in soy molasses supernatant, it was removed using ultrafiltration.The results showed that the trypsin inhibitor activity for soy molasses supernatant with about 10% solids content was approximately 5.05×105?TIA?g-1?protein, which was nearly 5 times of fresh soy milk whose trypsin inhibitor activity was about 1.026×105?TIA?g-1?protein. Protein with high molecular weight in soy molasses supernatant was concentrated by ultrafiltration using a membrane with a molecular weight cut-off of 3 kDa. The retentates showed one, unclear band with a molecular weight of about 10 kDa on the chromatogram of reduced Tricine.SDS-PAGE-After reduced by 2% mercaptoethanol and blocked thiol by NEM, the retentates of soy molasses supernatant showed two, clear bands with molecular weights of about 7.8 kDa Combined the facts, it was reasonable to consider that there was mainly Bowman-Birk trypsin inhibitor in soy molasses supernatant.Finally, ultrafiltrtion membrane with a molecular weight cut-off of 10 kDa was chosen to retain the trypsin inhibitor in soy molasses supernatant, and therefore permeates without inhibitor activity could be obtained.

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备注/Memo

基金项目：国家高技术发展研究计划“863计划”（2013AA102200）。

第一作者简介：石云（1984-），女，博士，主要从事大豆糖蜜相关研究。E-mail：shiyun0301@163.com。
通讯作者：华欲飞(1962-)，男，博士，教授，主要从事植物蛋白相关研究。E-mail：yfhua@jiangnan.edu.cn。

更新日期/Last Update: 2015-06-08