[1]李朝艳.萃取结晶法富集大豆油脚中的辅酶Q10[J].大豆科学,2014,33(06):920-923.[doi:10.11861/j.issn.1000-9841.2014.06.0920]
　LI Zhao-yan.Enrich Coenzyme Q10?from Soybean Oil Stocks by Extractive Crystallization Method[J].Soybean Science,2014,33(06):920-923.[doi:10.11861/j.issn.1000-9841.2014.06.0920]
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萃取结晶法富集大豆油脚中的辅酶Q10

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年06期 页码: 920-923 栏目: 出版日期: 2014-12-25
Title:
Enrich Coenzyme Q10?from Soybean Oil Stocks by Extractive Crystallization Method
文章编号:
1000-9841.2014.06.0920
作者:
李朝艳
Author(s):
LI Zhao-yan
Nanyang Institute of Technology School of Biological and Chemical Engineering,Nanyang 473004,China
关键词:
大豆油脚; 辅酶Q10; 萃取; 结晶
Keywords:
Soybean oil stocks; Coenzyme Q10; Extraction; Crystallization
分类号:
TQ645.9
DOI:
10.11861/j.issn.1000-9841.2014.06.0920
文献标志码:
A
摘要:
利用萃取结晶法从大豆油脚中富集辅酶Q10，通过高效液相色谱法对分离产物进行了检测，考察了萃取过程中正己烷、丙酮、乙醇、水用量以及结晶温度和时间对辅酶Q10回收率和纯度的影响。结果表明：最佳的工艺条件为100 g的大豆油脚，加300 mL正己烷分2次进行萃取，过滤。滤液加0.5 g NaCl盐析，分层，取油相旋转蒸发浓缩，回收正己烷，浓缩液中加100 mL丙酮分2次萃取，过滤，滤饼回收卵磷脂，滤液旋转蒸发，回收丙酮，油相加入100 mL 95%乙醇溶解，加入20 mL H2O，0℃结晶6 h。在该工艺条件下，辅酶Q10的回收率达到93.30%，纯度达到73.31%。
Abstract:
In this study,coenzyme Q10were enriched from soybean oil stocks by extractive crystallization method and the production were tested by HPLC.The suitable condition was:use 300 mL nhexane dissolve 100 g soybean bottom oil twice,filtrate it and add 0.5 g NaCl,separate organic phase,rotary evaporation to remove n-hexane,use 100 mL acetone extraction concentrate twice,filtrate,recycling lecithin from filter cake,rotary evaporation to remove acetone from liquid,mix 100 mL 95% ethanol to liquid,add 20 mL H2O,put mix liquid at 0℃,collect crystallization after 6 h.The results showed that the coenzyme Q10s purity was 73.31%,yield was 93.30% under the optimal condition.

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备注/Memo

作者简介：李朝艳（1980-），女，硕士，讲师，主要从事天然物提取和改性的研究。E-mail：zgwang2005@sohu.com。

更新日期/Last Update: 2014-12-29