[1]吴春雷,翟红,吴红艳,等.大豆极早熟品种60Co γ射线突变体库的建立及突变表型鉴定[J].大豆科学,2014,33(06):820-825.[doi:10.11861/j.issn.1000-9841.2014.06.0820]
　WU Chun-lei,ZHAI Hong,WU Hong-yan,et al.Identify of Mutant Phynotype and Construction of 60Co γ Mutant Population for Soybean Extremely Early Maturing Cultivar[J].Soybean Science,2014,33(06):820-825.[doi:10.11861/j.issn.1000-9841.2014.06.0820]
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大豆极早熟品种60Co γ射线突变体库的建立及突变表型鉴定

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年06期 页码: 820-825 栏目: 出版日期: 2014-12-25
Title:
Identify of Mutant Phynotype and Construction of 60Co γ Mutant Population for Soybean Extremely Early Maturing Cultivar
文章编号:
1000-9841.2014.06.0820
作者:
吴春雷1; 2; 翟红2; 吴红艳2; 柏锡1; 夏正俊2
1.东北农业大学 生命科学学院，黑龙江 哈尔滨 150030；
2.中国科学院 东北地理与农业生态研究所，黑龙江 哈尔滨 150081
Author(s):
WU Chun-lei1; 2; ZHAI Hong2; WU Hong-yan2; BAI Xi1; XIA Zheng-jun2
1. School of Life Science,Northeast Agricultural University,Harbin 150030,China；
2.Northeast Institute of Geography and Agroecology,Chinese Acadamy of Siences,Harbin 150081,China
关键词:
大豆突变体; 60Co γ射线; 黑河13; 北豆43
Keywords:
Soybean mutant; 60Co γ rays; Heihe 13; Beidou 43
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.06.0820
文献标志码:
A
摘要:
应用60Co γ射线对北方春大豆极早熟品种黑河13和北豆43种子进行诱变，并构建大豆突变体库。经M2代选择获得 420 份在株型、成熟期和育性等性状变异明显的材料，其中168份材料的变异与株高相关。通过对供体材料进行生育期基因型鉴定，在大豆生育期E1至E4基因的基因型分别是e1-as/e2/e3-T/E4，在M2代突变体库中获得了花期提前2～3 d的早花突变体38份，延迟开花6～8 d的晚花突变体50份。对选取的80份在M2表型有变异的材料进一步在M3代进行验证，获得25份与M2代表型一致的株行。同时随机选取385份M2代单株和株行的种子，通过近红外谷物分析仪对蛋白质和油分含量进行了测定，筛选比对照的供体材料蛋白质与油分含量明显偏高的变异材料15份。所获得的具有稳定遗传能力的材料可为研究相关性状的功能基因及分子育种提供较好的种质资源。
Abstract:
Soybean mutant population was constructed by treating the seeds of north spring soybean Heihe 13 and Beidou 43 with60Co γ rays.In M2generations,420 mutants of plant type,flowering and maturing time and fertility were obtained,168 plant height related mutants.By genotyping growth stage genes of two experimental varieties,the genotype of E1-E4was respective e1-as/e2/e3-T/E4,38 mutants with 2-3 d earlier flowering and 50 mutants with 6-8 d later flowering were obtained.In 80 mutants of M2,25 lines showed the same characters after validated in M3.After detected the contents of protein and oil of seeds from 385 M2?mutants（lines),there were 15 mutants with significant higher protein and oil content than control type were obtained.The mutants with stable genetic ability will be useful to study phenotype related functional genes and molecular soybean breeding.

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备注/Memo

基金项目：中国科学院百人计划及知识创新工程重要方向项目(KZCX2-EW-303)；国家自然科学基金(31271742)；黑龙江省重点项目(ZD201120)。

第一作者简介：吴春雷（1987-)，男，硕士，主要从事植物学研究。E-mail：wuchunlei0620@126.com。

更新日期/Last Update: 2014-12-25