[1]王罡,王娇,王萍,等.大豆两种外植体不定芽诱导能力的比较[J].大豆科学,2014,33(05):680-684.[doi:10.11861/j.issn.1000-9841.2014.05.0680]
　WANG Gang,WANG Jiao,WANG Ping,et al.Comparison on Adventitious Buds Induction Capacities of Two Kinds of Explants in Soybean[J].Soybean Science,2014,33(05):680-684.[doi:10.11861/j.issn.1000-9841.2014.05.0680]
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大豆两种外植体不定芽诱导能力的比较

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年05期 页码: 680-684 栏目: 出版日期: 2014-10-25
Title:
Comparison on Adventitious Buds Induction Capacities of Two Kinds of Explants in Soybean
文章编号:
100-9841.2014.05.0680
作者:
王罡1; 王娇2; 3; 王萍2; 季静1
1.天津大学 环境科学与工程学院，天津300072；
2.淮海工学院 海洋学院，江苏 连云港 222005；
3.江苏师范大学 生命科学院，江苏 徐州 221116
Author(s):
WANG Gang1; WANG Jiao2; 3; WANG Ping2; JI Jing1
1.School of Environmental Science and Engineering,Tianjin University,Tianjin 300072,China；
2.School of Marine Science and Technology,Huaihai Institute of Technology,Lianyungang 222005,China；
3.School of Life Science,Jiangsu Normal University,Xuzhou 221116,China
关键词:
大豆（Glycine max L.）; 胚尖; 下胚轴; 不定芽
Keywords:
Soybean（Glycine max L.）; Embryonic tip; Hypocotyl; Adventitious bud
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.05.0680
文献标志码:
A
摘要:
以大豆6个基因型的胚尖和下胚轴为外植体，研究了大豆不同基因型在胚尖、下胚轴两种再生体系中的不定芽诱导率、芽数和芽长情况，并比较了各基因型两种外植体的再生能力。结果表明：大豆不定芽的产生对基因型具有依赖性。在胚尖再生体系中，不定芽诱导率以吉林35较好；芽数以吉林35和合丰35较好；芽长以黑农44较好。在下胚轴再生体系中，不定芽诱导率以淮豆4号、黑农44、淮豆9号和吉林35较好；芽数除黑农40较差外，其他5个基因型相似；芽长以淮豆9号、黑农44和合丰35较好。综合不定芽诱导率、芽数、芽长比较两种外植体的再生能力可知，合丰35适合于胚尖和下胚轴两种再生体系，黑农40较适合于胚尖再生体系，淮豆9号较适合下胚轴再生体系。
Abstract:
To compare regeneration capacities of two kinds of explants in soybean (Glycine max L.),the induction rate,number and length of adventitious buds were investigated with embryonic tips and hypocotyls as explants.The results showed that the adventitious bud production depended on the genotypes applied in soybean.In the embryonic tip regeneration system,the maximum induction rate of adventitious buds was observed in Jilin 35,and the maximum number of adventitious buds was observed in Jinlin 35 and Hefeng 35,while as the longest adventitious buds appeared in Heinong 44.In the hypocotyls regeneration system,desired induction rates of adventitious buds were realized in Huaidou 4,Heinong 44,Huaidou 9 and Jinlin 35.Similar number of adventitious buds were achieved in all of those genotypes except for Heinong 40 possessing less adventitious buds.Furthermore,the maximum length of adventitious buds appeared in genotypes Huaidou 9,Heinong 44 and Hefeng 35.The results indicated that Hefeng 35 had the best regeneration capacity in both regeneration systems,based on the data from induction rates of adventitious buds,number and length of adventitious buds,and their regeneration capacities.In addition,Heinong 40 may to be more suitable for embryo tip regeneration system,and Huaidou 9 was more desirable for hypocotyl regeneration system.

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备注/Memo

基金项目：国家转基因生物新品种培育重大专项（2011ZX08004-001）。

第一作者简介：王罡（1964-），男，博士，教授，主要从事生物技术与植物转基因研究。E-mail:wanggangtjdx@126.com。
通讯作者：季静（1965-），女，博士，教授，主要从事植物基因工程与分子生物学研究。E-mail：jijingtjdx@163.com。

更新日期/Last Update: 2014-12-25