HUANG Gao-xia,ZHANG Li,WANG Dong-feng,et al.Soybean Trypsin Inhibitor Shows Anti-obesity Effect in High Fat Diet-Induced Obesity Mice[J].Soybean Science,2012,31(02):284-287.[doi:10.3969/j.issn.1000-9841.2012.02.026]
大豆胰蛋白酶抑制剂对饮食肥胖小鼠的减肥作用
- Title:
- Soybean Trypsin Inhibitor Shows Anti-obesity Effect in High Fat Diet-Induced Obesity Mice
- 文章编号:
- 1000-9841（2012）02-0284-04
- 关键词:
- 大豆胰蛋白酶抑制剂（SBTI）; 高脂饮食; 肥胖; 减肥
- 分类号:
- TS201.4
- 文献标志码:
- A
- 摘要:
- 采用断乳ICR小鼠为实验动物，第一阶段小鼠分为2组，正常小鼠组（NM）与肥胖模型小鼠组（FM），建立饮食肥胖小鼠模型；第二阶段NM作为肥胖预防组，FM作为肥胖治疗组，分别灌胃大豆胰蛋白酶抑制剂（soybean trypsin inhibitor,SBTI）。实验结束时，称体重，血液离心取得血清，测定甘油三酯（TG）、游离脂肪酸（FFA）、高密度脂蛋白（HDL-C）、总胆固醇（TCH）等指标，研究SBTI对饮食肥胖小鼠的减肥作用。结果表明：与预防对照组相比，肥胖预防组SBTI高剂量组的体重极显著降低（P<0.01），TG和FFA显著降低（P<0.05），HDL-C显著升高（P<0.05）；与模型对照组相比，肥胖治疗组SBTI高剂量组的体重极显著降低P<0.01），TG和FFA显著降低（P<0.05），HDL-C显著升高（P<0.05）；2组的TCH与相应对照组相比，差异均不显著（P＞0.05）。因此，SBTI对饮食肥胖有一定的预防和治疗作用。
- Abstract:
- One hundred and twenty weaning ICR mice were divided into 2 groups,normal mice(NM)and fat mice(FM)to establish high fat diet-induced obesity mice model.When the final weight of FM were higher than NM by 20%,NM and FM were regarded as obesity prevention and treatment group,respectively,and three dosages of SBTI were given by gavage for 42 d.At the end of the experiment,weight and triglyceride(TG),free fatty acid(FFA),high-density lipoprotein cholesterol(HDL-C),total cholesterol(TCH)were deterrnine to investigate the anti-obesity effect of SBTI in obesity model.The result showed that compared with prevention control group,weight,TG,FFA of the obesity prevention group with high dosage SBTI significantly decreased,HDL-C of that group significantly increased.Compared with model control group,weight,TG,FFA of the obesity treatment group with high dosage SBTI significantly decreased,HDL-C of that group significantly increased.The differences of TCH were not significant in both groups.So it suggests that SBTI is effective for obesity prevention and treatment.
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备注/Memo
基金项目：山东省自然科学基金重点项目(Z2004D05)；山东省自然科学基金项目(Q2008D11)；教育部博士点基金立项课题(20110132110007)。