LIU Jia,ZHANG Yun-hua,MENG Qing-lin,et al.Effect of Soil Bacteria in Soybean Rhizosphere to Fusarium [J].Soybean Science,2011,30(05):823-826.[doi:10.11861/j.issn.1000-9841.2011.05.0823]
大豆根际土壤细菌对镰孢菌的影响
- Title:
- Effect of Soil Bacteria in Soybean Rhizosphere to Fusarium
- 文章编号:
- 1000-9841（2011）05-0823-04
- Keywords:
- Bacteria in soil; Fusarium; Growth of Fusarium mycelial; Sporulation
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 采用室内生物学测定方法研究了大豆根际土壤细菌不同接菌量和接入时间对大豆根腐病病原菌镰孢菌（Fusarium）菌丝生长和产孢量的影响。结果表明：土壤细菌对镰孢菌菌丝生长和产孢量具有一定的抑制作用，接入细菌量越大，抑制率越高，当接入细菌量为5×106cfu·mL-1时，细菌对其菌丝生长及产孢抑制率分别达98.9%和98.3%；培养条件下细菌接入时间越早对尖镰孢菌和茄镰孢菌菌丝生长及产孢的抑制作用越明显。
- Abstract:
- In an indoor experiment,a biological determination was employed to compare the effect of bacteria,which was isolated from soybean rhizosphere soil,on mycelial growth and sporulation of Fusarium both with different inoculation amount and different access time.Results showed soil bacteria could depress mycelial growth and sporulation of Fusarium in a dose-dependent manner,when the bacteria amount was 5×106cfu·mL-1,the inhibitory rate of mycelial growth and sporulation reached 98.9% and 98.3%,respectively.The access time of bacteria also had significant influence on inhibitory of mycelial growth and sporulation of Fusarium,the earlier the access time,the obvious the inhibitory effect was.
参考文献/References:
[1]马汇泉,辛惠普.大豆根腐病病原菌种类鉴定及其生态学研究[J].黑龙江八一农垦大学学报,1988(2):115-121.(Ma H Q,Xin H P.Identification and ecology of pathogenic fungi of soybean root rot[J].Journal of Heilongjiang August First Land Reclamation University,1988(2):115-121.) [2]李春杰,许艳丽,赵志权,等.木霉颗粒剂对大豆根腐病的防治作用[J].大豆科学,2009,28(3):499-501.(Li C J,Xu Y L,Zhao Z Q,et al.Control effect of Trichoderma?sp.granules on soybean root rot[J].Soybean Science,2009,28(3):499-501.) [3]黄珊珊,韩雪,李丽珺,等.大豆根腐病生防菌株的筛选及鉴定[J].东北农业大学学报,2008,39(10):6-10.(Huang S S,Han X,Li L J,et al.Screening and identification of biocontrol strains against soybean root rot pathogens[J].Journal of Northeast Agricultural University,2008,39(10):6-10.) [4]徐佳,王士强,张兴梅.生物制剂拌种对盆栽大豆根腐病防效及土壤酶的影响[J].黑龙江八一农垦大学学报,2008,20(5):21-24.(Xu J,Wang S Q,Zhang X M.Effects of biological reagents in different seed dressing on the antagonism of soybean root rot and the mechanism[J].Journal of Heilongjiang August First Land Reclamation University,2008,20(5):21-24.) [5]张淑梅,王玉霞,赵晓宇,等.生物拌种剂防治大豆根腐病效果和机制[J].大豆科学,2009,28(15):864-868.(Zhang S M,Wang Y X,Zhao X Y,et al.Efficacy and mechanism of biological seed coating agent against soybean root rot disease caused by Fusarium oxysporum?f.sp.vedolenst[J].Soybean Science,2009,28(15):864-868.) [6]徐诚蛟,徐凤花,万书明,等.拮抗菌株对田间大豆根腐病原菌抑制效果[J].大豆科学,2008,27(2):351-353.(Xu C J,Xu F H,Wan S M,et al.Inhibitory effect of antagonisms on pathogen of soybean root rot in field[J].Soybean Science,2008,27(2):351-353.) [7]孙冬梅,杨谦,宋金柱.黄绿木霉菌对大豆根腐病镰刀菌的拮抗作用[J].中国油料作物学报,2005,27(3):58-62.(Sun D M,Yang Q,Song J Z.Antagonism of Trichoderma?aureoviride against Fusarium?spp which causes soybean root rot[J].Chinese Journal of Oil Crop Sciences,2005,27(3):58-62.) [8]陈立杰,段玉玺,王媛媛,等.不同细菌菌株对大豆根腐病菌及胞囊线虫病的影响[J].沈阳农业大学学报,2006,37(6):831-834.(Chen L J,Duan Y X,Wang Y Y,et al.Bio-effect of different bacterial strains on soybean root rot pathogens and Heterodera glycines[J].Journal of Shenyang Agricultural University,2006,37(6):831-834.) [9]王媛媛,段玉玺,陈立杰.不同细菌菌株对大豆根腐病菌及胞囊线虫病的影响[J].大豆科学,2007,26(2):213-217.(Wang Y Y,Duan Y X,Chen L J.Effect of endophytic bacteria from soybean root nodules on soybean cystnematode and pathogens of soybean root rot[J].Soybean Science,2007,26(2):213-217.) [10]许艳丽,张红骥,张匀华,等.复配生防菌株防治大豆根腐病的研究[J].大豆科学,2008,27(2):270-274.(Xu Y L,Zhang H J,Zhang Y H,et al.Combining biocontrol strains against soybean root rot[J].Soybean Science,2008,27(2):270-274.) [11]白剑宇,白丽燕,王登元,等.大豆根腐病病原菌PCR-RFLP鉴定体系的建立[J].新疆农业大学学报,2010,33(2):151-154.(Bai J Y,Bai L Y,Wang D Y,et al.Establishment of PCR-RFLP identification system of soybean root rot pathogen[J].Journal of Xinjiang Agricultural University,2010,33(2):151-154.)
相似文献/References:
[1]魏巍,许艳丽,刘金波,等.土壤镰孢菌Real-Time QPCR定量方法的建立及应用[J].大豆科学,2010,29(04):655.[doi:10.11861/j.issn.1000-9841.2010.04.0655]
WEI Wei,XU Yan-li,LIU Jin-bo,et al.Development and Application of a Real-Time Quantitative PCR Assay for Detection of Fusarium spp. in Soil[J].Soybean Science,2010,29(05):655.[doi:10.11861/j.issn.1000-9841.2010.04.0655]
[2]刘金波,许艳丽,吕国忠,等.黑土区不同轮作系统大豆根际镰孢菌种群结构和数量[J].大豆科学,2009,28(01):97.[doi:10.11861/j.issn.1000-9841.2009.01.0097]
LIU Jin-bo,XU Yan-li,L Guo-zhong,et al.Black Soil Region Fusarium Population Structure and Quantity in Soybean Rhizosphere of Different Rotation System[J].Soybean Science,2009,28(05):97.[doi:10.11861/j.issn.1000-9841.2009.01.0097]
[3]刘金波,许艳丽,魏巍.大豆根际土壤镰孢菌不同分离方法比较[J].大豆科学,2008,27(01):106.[doi:10.11861/j.issn.1000-9841.2008.01.0106]
LIU Jin-bo,XU Yan-li,and WEI Wei.Comparing Different Methods for Isolating Fusarium from Soybean Rhizosphere Soil[J].Soybean Science,2008,27(05):106.[doi:10.11861/j.issn.1000-9841.2008.01.0106]
[4]杨晓贺,张瑜,丁俊杰,等.黑龙江省东部地区大豆根腐病调查研究初报[J].大豆科学,2015,34(06):1093.[doi:10.11861/j.issn.1000-9841.2015.06.1093]
YANG Xiao-he,ZHANG Yu,DING Jun-jie,et al.Preliminary Investigation Report on Soybean Root Rot in the Eastern of Heilongjiang Province[J].Soybean Science,2015,34(05):1093.[doi:10.11861/j.issn.1000-9841.2015.06.1093]
[5]桑永生,刘月,袁翠平,等.吉林省公主岭与敦化大豆根腐病镰孢菌的系统鉴定[J].大豆科学,2020,39(03):442.[doi:10.11861/j.issn.1000-9841.2020.03.0442]
SANG Yong-sheng,LIU Yue,YUAN Cui-ping,et al.Systematic Identification of Fusarium Species Associated with Soybean Root Rot in Dunhua and Gongzhuling, Jilin Province[J].Soybean Science,2020,39(05):442.[doi:10.11861/j.issn.1000-9841.2020.03.0442]
[6]袁素娟,焦晓丹,郑素娇,等.2021年黑龙江省疑似大豆猝死综合症的病原鉴定[J].大豆科学,2023,42(04):451.[doi:10.11861/j.issn.1000-9841.2023.04.0451]
备注/Memo
第一作者简介：刘佳（1986-），男，研究实习员，硕士，研究方向为植物土传病害。E-mail：liujia4218@163.com。