CAI Yan,FANG Yun,XIA Yong-mei.Thermal Inactivation of Soybean Lipoxygenase and Its Secondary Structure Performance of Circular Dichroism Specctroscopy[J].Soybean Science,2011,30(01):150-152.[doi:10.11861/j.issn.1000-9841.2011.01.0150]
大豆脂氧酶的热失活动力学与其二级结构的圆二色谱表现
- Title:
- Thermal Inactivation of Soybean Lipoxygenase and Its Secondary Structure Performance of Circular Dichroism Specctroscopy
- 文章编号:
- 1000-9841（2011）01-0150-03
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 应用大豆脂氧酶的变温圆二色谱图研究了大豆脂氧酶(LOX)的热失活与二级结构变化过程。结果表明：在50~65℃条件下，10 min内，LOX的酶活随热处理时间延长而下降，其热失活过程遵循一级动力学，活化能Ea值为217 kJ·mol-1。在相同的热处理条件下，LOX中二级结构α-螺旋和β-折叠的相对含量均在20%左右浮动，不符合一级反应动力学，二级结构的变化并非LOX失活的主要原因。
- Abstract:
- The inactivation and secondary structure variation of soybean lipoxygenase(LOX) during heating process was studied with circular dichroism(CD) spectroscopy. The quantitative secondary structures of LOX at various temperatures were obtained. At 50~65 ℃, LOX activity has a sharp drop with the time prolonging. Thermal inactivation of LOX could be described by a first order kinetic model, and activation energy Ea was further calculated to be 217 kJ·mol-1. During the heating process, the contents of α-helix and β-sheet were both floating at 20%, indicated that the change of secondary structure of LOX was not the main reason for the loss of their activities.
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备注/Memo
基金项目：江南大学创新团队基金资助项目(2008CXTD01)。